TF2H4_DICDI
ID TF2H4_DICDI Reviewed; 483 AA.
AC Q54C29;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=General transcription factor IIH subunit 4;
DE AltName: Full=TFIIH basal transcription factor complex subunit 4;
GN Name=gtf2h4; Synonyms=tfiih4; ORFNames=DDB_G0293228;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Component of the general transcription and DNA repair factor
CC IIH (TFIIH) core complex, which is involved in general and
CC transcription-coupled nucleotide excision repair (NER) of damaged DNA
CC and, when complexed to CAK, in RNA transcription by RNA polymerase II.
CC In NER, TFIIH acts by opening DNA around the lesion to allow the
CC excision of the damaged oligonucleotide and its replacement by a new
CC DNA fragment. In transcription, TFIIH has an essential role in
CC transcription initiation. When the pre-initiation complex (PIC) has
CC been established, TFIIH is required for promoter opening and promoter
CC escape. Phosphorylation of the C-terminal tail (CTD) of the largest
CC subunit of RNA polymerase II by the kinase module CAK controls the
CC initiation of transcription. {ECO:0000250|UniProtKB:Q92759}.
CC -!- SUBUNIT: Component of the 7-subunit TFIIH core complex composed of
CC XPB/repB, XPD/repD, gtf2h1, gtf2h2, gtf2h3, gtf2h4 and gtf2h5, which is
CC active in NER. The core complex associates with the 3-subunit CDK-
CC activating kinase (CAK) module composed of cycH/cyclin H, cdk7 and
CC mnat1 to form the 10-subunit holoenzyme (holo-TFIIH) active in
CC transcription. {ECO:0000250|UniProtKB:Q92759}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TFB2 family. {ECO:0000305}.
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DR EMBL; AAFI02000200; EAL60813.1; -; Genomic_DNA.
DR RefSeq; XP_629237.1; XM_629235.1.
DR AlphaFoldDB; Q54C29; -.
DR SMR; Q54C29; -.
DR STRING; 44689.DDB0231037; -.
DR PaxDb; Q54C29; -.
DR EnsemblProtists; EAL60813; EAL60813; DDB_G0293228.
DR GeneID; 8629119; -.
DR KEGG; ddi:DDB_G0293228; -.
DR dictyBase; DDB_G0293228; gtf2h4.
DR eggNOG; KOG3471; Eukaryota.
DR HOGENOM; CLU_027280_4_0_1; -.
DR InParanoid; Q54C29; -.
DR OMA; LWENERN; -.
DR PhylomeDB; Q54C29; -.
DR Reactome; R-DDI-113418; Formation of the Early Elongation Complex.
DR Reactome; R-DDI-5696395; Formation of Incision Complex in GG-NER.
DR Reactome; R-DDI-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-DDI-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-DDI-6782135; Dual incision in TC-NER.
DR Reactome; R-DDI-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-DDI-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-DDI-72086; mRNA Capping.
DR Reactome; R-DDI-73772; RNA Polymerase I Promoter Escape.
DR Reactome; R-DDI-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-DDI-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-DDI-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-DDI-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-DDI-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-DDI-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR PRO; PR:Q54C29; -.
DR Proteomes; UP000002195; Chromosome 6.
DR GO; GO:0000439; C:transcription factor TFIIH core complex; IBA:GO_Central.
DR GO; GO:0005675; C:transcription factor TFIIH holo complex; ISS:dictyBase.
DR GO; GO:0001671; F:ATPase activator activity; IEA:InterPro.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0006289; P:nucleotide-excision repair; ISS:dictyBase.
DR GO; GO:0070816; P:phosphorylation of RNA polymerase II C-terminal domain; IBA:GO_Central.
DR GO; GO:0006366; P:transcription by RNA polymerase II; ISS:dictyBase.
DR InterPro; IPR040662; Tfb2_C.
DR InterPro; IPR004598; TFIIH_p52/Tfb2.
DR PANTHER; PTHR13152; PTHR13152; 1.
DR Pfam; PF03849; Tfb2; 1.
DR Pfam; PF18307; Tfb2_C; 1.
PE 3: Inferred from homology;
KW DNA damage; DNA repair; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..483
FT /note="General transcription factor IIH subunit 4"
FT /id="PRO_0000327568"
FT REGION 93..117
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 483 AA; 55726 MW; 81E809ACE47AE44E CRC64;
MTLSKVFQYL ASLDSKDLEE LYKDPWTCQA ILRSLPPRSK QYILKMLLVD TYPLSLAKDW
STQASIQQHK ESLKKLFDLK IIFLDKINKP IQPQQQQSSQ QSSSQQQQQQ QQQQQQTEQT
IRLNPLFQDN IKRSLVQVNQ VIFSNNSSIK DNHKPPSIDD LDSYSKSQWE KVLYFLSDDT
VQPSKLISEL LLSSNLTKQE GDGLSITSEG FKFLLKDVYT QIWTLLIVYL DDLEKKKGKG
SGSRNDLLSF LFRLSFLNLG RGYLVSELSE QQKEYLFALK QFGLIYMRTD SSILFYPTRL
IISLTTGKTL SLIQSISSER TQTQKEQGYI VLETNYRLYA YTSSSLQISL LSLFVKMLYR
LPNLAVGIIT RESIRTALIH GITADQIIDF VRHNSHPNAA NSGQPIPDVV AEQILLWEAE
RNRITYTKSV LYNSFPTNDC YIATLKFAKE QDYYIWSHDP LKTLVVKEEG NDPIRNFIKK
NFA
