TF2H4_MOUSE
ID TF2H4_MOUSE Reviewed; 463 AA.
AC O70422;
DT 18-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=General transcription factor IIH subunit 4;
DE AltName: Full=Basic transcription factor 2 52 kDa subunit;
DE Short=BTF2 p52;
DE AltName: Full=General transcription factor IIH polypeptide 4;
DE AltName: Full=TFIIH basal transcription factor complex p52 subunit;
GN Name=Gtf2h4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J; TISSUE=T-cell lymphoma;
RA Lanning D., Lafuse W.P.;
RT "The mouse p52 subunit of the transcription/DNA repair factor TFIIH is
RT located in the class III region of the H-2 complex: cloning and sequence
RT polymorphism.";
RL Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the general transcription and DNA repair factor
CC IIH (TFIIH) core complex, which is involved in general and
CC transcription-coupled nucleotide excision repair (NER) of damaged DNA
CC and, when complexed to CAK, in RNA transcription by RNA polymerase II.
CC In NER, TFIIH acts by opening DNA around the lesion to allow the
CC excision of the damaged oligonucleotide and its replacement by a new
CC DNA fragment. In transcription, TFIIH has an essential role in
CC transcription initiation. When the pre-initiation complex (PIC) has
CC been established, TFIIH is required for promoter opening and promoter
CC escape. Phosphorylation of the C-terminal tail (CTD) of the largest
CC subunit of RNA polymerase II by the kinase module CAK controls the
CC initiation of transcription. {ECO:0000250|UniProtKB:Q92759}.
CC -!- SUBUNIT: Component of the 7-subunit TFIIH core complex composed of
CC XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which
CC is active in NER. The core complex associates with the 3-subunit CDK-
CC activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and
CC MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in
CC transcription. {ECO:0000250|UniProtKB:Q92759}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the TFB2 family. {ECO:0000305}.
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DR EMBL; AF054823; AAC08594.1; -; mRNA.
DR EMBL; BC012638; AAH12638.1; -; mRNA.
DR CCDS; CCDS28702.1; -.
DR RefSeq; NP_034494.1; NM_010364.4.
DR RefSeq; XP_006523731.1; XM_006523668.3.
DR RefSeq; XP_006523732.1; XM_006523669.3.
DR AlphaFoldDB; O70422; -.
DR SMR; O70422; -.
DR BioGRID; 200112; 2.
DR CORUM; O70422; -.
DR IntAct; O70422; 1.
DR STRING; 10090.ENSMUSP00000001565; -.
DR iPTMnet; O70422; -.
DR PhosphoSitePlus; O70422; -.
DR EPD; O70422; -.
DR PaxDb; O70422; -.
DR PeptideAtlas; O70422; -.
DR PRIDE; O70422; -.
DR ProteomicsDB; 263112; -.
DR Antibodypedia; 48243; 191 antibodies from 26 providers.
DR DNASU; 14885; -.
DR Ensembl; ENSMUST00000001565; ENSMUSP00000001565; ENSMUSG00000001524.
DR Ensembl; ENSMUST00000160734; ENSMUSP00000124335; ENSMUSG00000001524.
DR GeneID; 14885; -.
DR KEGG; mmu:14885; -.
DR UCSC; uc008cig.2; mouse.
DR CTD; 2968; -.
DR MGI; MGI:1338799; Gtf2h4.
DR VEuPathDB; HostDB:ENSMUSG00000001524; -.
DR eggNOG; KOG3471; Eukaryota.
DR GeneTree; ENSGT00390000014159; -.
DR HOGENOM; CLU_027280_4_0_1; -.
DR InParanoid; O70422; -.
DR OMA; LWENERN; -.
DR OrthoDB; 466731at2759; -.
DR PhylomeDB; O70422; -.
DR TreeFam; TF300879; -.
DR Reactome; R-MMU-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-MMU-113418; Formation of the Early Elongation Complex.
DR Reactome; R-MMU-5696395; Formation of Incision Complex in GG-NER.
DR Reactome; R-MMU-5696400; Dual Incision in GG-NER.
DR Reactome; R-MMU-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-MMU-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-MMU-6782135; Dual incision in TC-NER.
DR Reactome; R-MMU-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-MMU-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-MMU-72086; mRNA Capping.
DR Reactome; R-MMU-73762; RNA Polymerase I Transcription Initiation.
DR Reactome; R-MMU-73772; RNA Polymerase I Promoter Escape.
DR Reactome; R-MMU-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-MMU-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-MMU-73863; RNA Polymerase I Transcription Termination.
DR Reactome; R-MMU-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-MMU-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-MMU-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-MMU-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR BioGRID-ORCS; 14885; 19 hits in 112 CRISPR screens.
DR PRO; PR:O70422; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; O70422; protein.
DR Bgee; ENSMUSG00000001524; Expressed in thymus and 267 other tissues.
DR ExpressionAtlas; O70422; baseline and differential.
DR Genevisible; O70422; MM.
DR GO; GO:0000438; C:core TFIIH complex portion of holo TFIIH complex; ISS:UniProtKB.
DR GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005669; C:transcription factor TFIID complex; ISO:MGI.
DR GO; GO:0000439; C:transcription factor TFIIH core complex; IBA:GO_Central.
DR GO; GO:0005675; C:transcription factor TFIIH holo complex; ISS:UniProtKB.
DR GO; GO:0001671; F:ATPase activator activity; IEA:InterPro.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; ISO:MGI.
DR GO; GO:0006289; P:nucleotide-excision repair; IBA:GO_Central.
DR GO; GO:0070816; P:phosphorylation of RNA polymerase II C-terminal domain; IBA:GO_Central.
DR GO; GO:0006366; P:transcription by RNA polymerase II; ISS:UniProtKB.
DR InterPro; IPR040662; Tfb2_C.
DR InterPro; IPR004598; TFIIH_p52/Tfb2.
DR PANTHER; PTHR13152; PTHR13152; 1.
DR Pfam; PF03849; Tfb2; 1.
DR Pfam; PF18307; Tfb2_C; 1.
DR TIGRFAMs; TIGR00625; tfb2; 1.
PE 1: Evidence at protein level;
KW DNA damage; DNA repair; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..463
FT /note="General transcription factor IIH subunit 4"
FT /id="PRO_0000119254"
SQ SEQUENCE 463 AA; 52224 MW; DBAA15CB388C5AFC CRC64;
MEITPARGGL NRAHLQCRNL QEFLGGLSPG VLDRLYGHPA TCLAVFRELP SLAKNWVMRM
LFLEQPLPQA AVALWVKKEF SKAQEESTGL LSGLRIWHTQ LLPGGLQGLI LNPVFRQNLR
IALLGGGKAW SDDTSQLGPD KHARDVPSLD KYAEERWEVV LHFMVGSPSA AVSQDLAQLL
SQAGLMKSTE PGEPPCITSA GFQFLLLDTP AQLWYFMLQY LQTAQSRGMD LVEILSFLFQ
LSFSTLGKDY SVEGMSDSLL NFLQHLREFG LVFQRKRKSR RYYPTRLAIN LSSGVSGAGG
TVHQPGFIVV ETNYRLYAYT ESELQIALIA LFSEMLYRFP NMVVAQVTRE SVQQAIASGI
TAQQIIHFLR TRAHPVMLKQ NPVLPPTITD QIRLWELERD RLRFTEGVLY NQFLSQVDFE
LLLAHARELG VLVFENSAKR LMVVTPAGHS DVKRFWKRQK HSS
