TF2H5_CAEEL
ID TF2H5_CAEEL Reviewed; 71 AA.
AC Q9N390;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=General transcription and DNA repair factor IIH subunit TFB5 {ECO:0000255|RuleBase:RU368032};
DE AltName: Full=TFIIH subunit GTF-2H5 {ECO:0000303|PubMed:34824371};
GN Name=gtf-2H5 {ECO:0000312|WormBase:Y55B1AL.2};
GN ORFNames=Y55B1AL.2 {ECO:0000312|WormBase:Y55B1AL.2};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=34824371; DOI=10.1038/s42003-021-02875-8;
RA Thijssen K.L., van der Woude M., Davo-Martinez C., Dekkers D.H.W.,
RA Sabatella M., Demmers J.A.A., Vermeulen W., Lans H.;
RT "C. elegans TFIIH subunit GTF-2H5/TTDA is a non-essential transcription
RT factor indispensable for DNA repair.";
RL Commun. Biol. 4:1336-1336(2021).
CC -!- FUNCTION: Component of the general transcription and DNA repair factor
CC IIH (TFIIH) core complex, which is involved in general and
CC transcription-coupled nucleotide excision repair (NER) of damaged DNA
CC and in RNA transcription by RNA polymerase II (By similarity). In NER,
CC TFIIH acts by opening DNA around the lesion to allow the excision of
CC the damaged oligonucleotide and its replacement by a new DNA fragment
CC (By similarity). In transcription, TFIIH has an essential role in
CC transcription initiation (By similarity). When the pre-initiation
CC complex (PIC) has been established, TFIIH is required for promoter
CC opening and promoter escape (By similarity). Necessary for the
CC stability of the TFIIH complex and for the presence of normal levels of
CC TFIIH in the cell (PubMed:34824371). Required for efficient binding of
CC TFIIH to damaged DNA (PubMed:34824371). Dispensable for normal
CC development, but required when transcription is challenged
CC (PubMed:34824371). {ECO:0000250|UniProtKB:Q6ZYL4,
CC ECO:0000255|RuleBase:RU368032, ECO:0000269|PubMed:34824371}.
CC -!- SUBUNIT: Component of the 7-subunit TFIIH core complex.
CC {ECO:0000255|RuleBase:RU368032}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|RuleBase:RU368032,
CC ECO:0000269|PubMed:34824371}. Chromosome {ECO:0000269|PubMed:34824371}.
CC Note=Localized to paired homologous chromosomes (bivalents) about 10
CC minutes after UV irradiation, but localization is lost after 35
CC minutes, probably as a result of successful DNA damage repair as part
CC of the TFIIH complex. {ECO:0000269|PubMed:34824371}.
CC -!- DISRUPTION PHENOTYPE: Auxin-induced degradation of the protein causes
CC no obvious phenotype in germ cells and early embryos.
CC {ECO:0000269|PubMed:34824371}.
CC -!- SIMILARITY: Belongs to the TFB5 family.
CC {ECO:0000255|RuleBase:RU368032}.
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DR EMBL; BX284603; CCD61809.1; -; Genomic_DNA.
DR RefSeq; NP_497212.1; NM_064811.5.
DR AlphaFoldDB; Q9N390; -.
DR SMR; Q9N390; -.
DR DIP; DIP-26454N; -.
DR STRING; 6239.Y55B1AL.2; -.
DR EPD; Q9N390; -.
DR PaxDb; Q9N390; -.
DR PeptideAtlas; Q9N390; -.
DR EnsemblMetazoa; Y55B1AL.2.1; Y55B1AL.2.1; WBGene00021904.
DR GeneID; 190298; -.
DR KEGG; cel:CELE_Y55B1AL.2; -.
DR UCSC; Y55B1AL.2; c. elegans.
DR CTD; 190298; -.
DR WormBase; Y55B1AL.2; CE22497; WBGene00021904; gtf-2H5.
DR eggNOG; KOG3451; Eukaryota.
DR GeneTree; ENSGT00390000004028; -.
DR HOGENOM; CLU_166246_4_0_1; -.
DR InParanoid; Q9N390; -.
DR OMA; IMENMNP; -.
DR OrthoDB; 1591321at2759; -.
DR PhylomeDB; Q9N390; -.
DR Reactome; R-CEL-112382; Formation of RNA Pol II elongation complex.
DR Reactome; R-CEL-113418; Formation of the Early Elongation Complex.
DR Reactome; R-CEL-5696395; Formation of Incision Complex in GG-NER.
DR Reactome; R-CEL-5696400; Dual Incision in GG-NER.
DR Reactome; R-CEL-674695; RNA Polymerase II Pre-transcription Events.
DR Reactome; R-CEL-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-CEL-6782135; Dual incision in TC-NER.
DR Reactome; R-CEL-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR Reactome; R-CEL-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR Reactome; R-CEL-72086; mRNA Capping.
DR Reactome; R-CEL-73772; RNA Polymerase I Promoter Escape.
DR Reactome; R-CEL-73776; RNA Polymerase II Promoter Escape.
DR Reactome; R-CEL-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR Reactome; R-CEL-75953; RNA Polymerase II Transcription Initiation.
DR Reactome; R-CEL-75955; RNA Polymerase II Transcription Elongation.
DR Reactome; R-CEL-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR Reactome; R-CEL-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00021904; Expressed in embryo and 4 other tissues.
DR GO; GO:0000439; C:transcription factor TFIIH core complex; IBA:GO_Central.
DR GO; GO:0005675; C:transcription factor TFIIH holo complex; IBA:GO_Central.
DR GO; GO:0006294; P:nucleotide-excision repair, preincision complex assembly; IBA:GO_Central.
DR GO; GO:0070816; P:phosphorylation of RNA polymerase II C-terminal domain; IBA:GO_Central.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.70.1220; -; 1.
DR InterPro; IPR035935; TFB5-like_sf.
DR InterPro; IPR009400; TFIIH_TTDA/Tfb5.
DR PANTHER; PTHR28580; PTHR28580; 1.
DR Pfam; PF06331; Tfb5; 1.
DR SMART; SM01395; Tbf5; 1.
DR SUPFAM; SSF142897; SSF142897; 1.
PE 3: Inferred from homology;
KW Chromosome; DNA damage; DNA repair; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..71
FT /note="General transcription and DNA repair factor IIH
FT subunit TFB5"
FT /id="PRO_0000456248"
SQ SEQUENCE 71 AA; 8207 MW; 2566E1355CB51139 CRC64;
MVNVKKGVLV TSDPAFRQLL IHLDDSRQLG SKFIVRELDD THLFIEKEIV PMLENKVEQI
MENMNPEAVD K
