TF3B_CANAL
ID TF3B_CANAL Reviewed; 553 AA.
AC P43072; A0A1D8PT19; Q59PR5;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Transcription factor IIIB 70 kDa subunit;
DE Short=TFIIIB;
DE AltName: Full=B-related factor 1;
DE Short=BRF-1;
GN Name=TDS4; Synonyms=BRF, BRF1; OrderedLocusNames=CAALFM_CR05650WA;
GN ORFNames=CaO19.13970, CaO19.6649;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7995525; DOI=10.1101/gad.8.23.2879;
RA Khoo B., Brophy B., Jackson S.P.;
RT "Conserved functional domains of the RNA polymerase III general
RT transcription factor BRF.";
RL Genes Dev. 8:2879-2890(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC -!- FUNCTION: General activator of RNA polymerase III transcription.
CC Interacts with TBP. Binds to Pol III subunit C34 and to the TAU135
CC component of TFIIIC.
CC -!- SUBUNIT: TFIIIB comprises the TATA-binding protein (TBP), the B-related
CC factor (BRF) and a 70 kDa polypeptide.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the TFIIB family. {ECO:0000305}.
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DR EMBL; Z47202; CAA87398.1; -; Genomic_DNA.
DR EMBL; CP017630; AOW31281.1; -; Genomic_DNA.
DR PIR; B55483; B55483.
DR RefSeq; XP_711715.1; XM_706623.1.
DR AlphaFoldDB; P43072; -.
DR SMR; P43072; -.
DR STRING; 237561.P43072; -.
DR PRIDE; P43072; -.
DR GeneID; 3646689; -.
DR KEGG; cal:CAALFM_CR05650WA; -.
DR CGD; CAL0000177227; BRF1.
DR VEuPathDB; FungiDB:CR_05650W_A; -.
DR eggNOG; KOG1598; Eukaryota.
DR HOGENOM; CLU_010293_3_3_1; -.
DR InParanoid; P43072; -.
DR OMA; NYEALDM; -.
DR OrthoDB; 412601at2759; -.
DR PRO; PR:P43072; -.
DR Proteomes; UP000000559; Chromosome R.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000126; C:transcription factor TFIIIB complex; ISS:CGD.
DR GO; GO:0097550; C:transcription preinitiation complex; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000995; F:RNA polymerase III general transcription initiation factor activity; IBA:GO_Central.
DR GO; GO:0001006; F:RNA polymerase III type 3 promoter sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0017025; F:TBP-class protein binding; IEA:InterPro.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IBA:GO_Central.
DR GO; GO:0006383; P:transcription by RNA polymerase III; IBA:GO_Central.
DR GO; GO:0006384; P:transcription initiation from RNA polymerase III promoter; ISS:CGD.
DR GO; GO:0070897; P:transcription preinitiation complex assembly; IEA:InterPro.
DR CDD; cd00043; CYCLIN; 2.
DR InterPro; IPR029529; Brf1.
DR InterPro; IPR011665; BRF1_TBP-bd_dom.
DR InterPro; IPR013763; Cyclin-like.
DR InterPro; IPR036915; Cyclin-like_sf.
DR InterPro; IPR000812; TFIIB.
DR InterPro; IPR023486; TFIIB_CS.
DR InterPro; IPR013150; TFIIB_cyclin.
DR InterPro; IPR013137; Znf_TFIIB.
DR PANTHER; PTHR11618; PTHR11618; 1.
DR PANTHER; PTHR11618:SF4; PTHR11618:SF4; 1.
DR Pfam; PF07741; BRF1; 1.
DR Pfam; PF08271; TF_Zn_Ribbon; 1.
DR Pfam; PF00382; TFIIB; 2.
DR PRINTS; PR00685; TIFACTORIIB.
DR SMART; SM00385; CYCLIN; 2.
DR SUPFAM; SSF47954; SSF47954; 2.
DR PROSITE; PS00782; TFIIB; 2.
DR PROSITE; PS51134; ZF_TFIIB; 1.
PE 3: Inferred from homology;
KW Activator; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..553
FT /note="Transcription factor IIIB 70 kDa subunit"
FT /id="PRO_0000119343"
FT REPEAT 98..174
FT /note="1"
FT REPEAT 193..272
FT /note="2"
FT ZN_FING 6..39
FT /note="TFIIB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT REGION 98..272
FT /note="Interaction with TBP and with the Pol III subunit
FT C34"
FT REGION 281..553
FT /note="Interaction with TBP"
FT REGION 473..523
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 495..520
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 10
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 13
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 31
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 34
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
SQ SEQUENCE 553 AA; 61840 MW; FEC1E270B15EEEC1 CRC64;
MSKPRKQQKC KTCGHTQFDV NRYTAAGDVS CLRCGTVLEE NPIVSEVQFG ESSSGAAMVQ
GAMVGADQAR ATFAGGRQNA MESREQTLSN GKRKIKRIAA ALKIPDYIAE AAGEWFRLAL
TLNFVQGRRS NNVLATCLYV ACRKERTHHM LIDFSSRLQI SVYSLGATFL KMVKALHITS
LPLADPSLFI QHFVEKLDFK DKATKVAKDA VKLAHRMAAD WIHEGRRPAG IAGACVLLAA
RMNNFRRSHA EIVAVSHVGE ETLQRRLNEF KKTKAGTLSV KSFREVENLE SSNPPSFEKN
RAMELKISKK LQQQQTDNFE DLSKMTEEEK QSVFGKLSKE EAQKQLLMNT ILSDITITTE
NLNDQMDRIL KMKKSSLENS LYKTPYELAL ANGSEQDPSK IWNINKPKNL VANLPKTDDI
LQNVSSEVEL NSDDDDEIVL ESKLTEEEVA IKERIWTGLN HDYLVEQEKK RLKQEADELT
GNTSKSSSGN RRKRNKSSLP AELRKELGDI DLDEDGTPRS AADSAKMYIS KTSVSKKINY
DSLKGLLGGN MGF
