TF3B_SCHPO
ID TF3B_SCHPO Reviewed; 492 AA.
AC Q9P6R0; Q9USU4;
DT 16-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2012, sequence version 2.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Transcription factor IIIB 60 kDa subunit;
DE Short=TFIIIB;
DE AltName: Full=B-related factor 1;
DE Short=BRF-1;
DE AltName: Full=TFIIB-related factor;
GN Name=brf1; ORFNames=SPBC13E7.10c, SPBC30D10.20;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP REVISION OF GENE MODEL.
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
RN [3]
RP FUNCTION, AND INTERACTION WITH MAF1.
RX PubMed=15590667; DOI=10.1074/jbc.m412375200;
RA Desai N., Lee J., Upadhya R., Chu Y., Moir R.D., Willis I.M.;
RT "Two steps in Maf1-dependent repression of transcription by RNA polymerase
RT III.";
RL J. Biol. Chem. 280:6455-6462(2005).
RN [4]
RP INTERACTION WITH TBP.
RX PubMed=12682361; DOI=10.1093/nar/gkg301;
RA Huang Y., McGillicuddy E., Weindel M., Dong S., Maraia R.J.;
RT "The fission yeast TFIIB-related factor limits RNA polymerase III to a
RT TATA-dependent pathway of TBP recruitment.";
RL Nucleic Acids Res. 31:2108-2116(2003).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: General activator of RNA polymerase III transcription.
CC {ECO:0000269|PubMed:15590667}.
CC -!- SUBUNIT: TFIIIB comprises the TATA-binding protein (TBP), the B-related
CC factor (BRF) and a third subunit (Potential). Interacts with maf1.
CC {ECO:0000269|PubMed:12682361, ECO:0000269|PubMed:15590667,
CC ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the TFIIB family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CU329671; CAB89885.2; -; Genomic_DNA.
DR PIR; T40176; T40176.
DR RefSeq; NP_596265.2; NM_001022185.2.
DR AlphaFoldDB; Q9P6R0; -.
DR SMR; Q9P6R0; -.
DR BioGRID; 276609; 9.
DR STRING; 4896.SPBC13E7.10c.1; -.
DR iPTMnet; Q9P6R0; -.
DR MaxQB; Q9P6R0; -.
DR PaxDb; Q9P6R0; -.
DR PRIDE; Q9P6R0; -.
DR EnsemblFungi; SPBC13E7.10c.1; SPBC13E7.10c.1:pep; SPBC13E7.10c.
DR PomBase; SPBC13E7.10c; brf1.
DR VEuPathDB; FungiDB:SPBC13E7.10c; -.
DR eggNOG; KOG1598; Eukaryota.
DR HOGENOM; CLU_010293_2_3_1; -.
DR InParanoid; Q9P6R0; -.
DR OMA; NYEALDM; -.
DR Reactome; R-SPO-76061; RNA Polymerase III Transcription Initiation From Type 1 Promoter.
DR Reactome; R-SPO-76066; RNA Polymerase III Transcription Initiation From Type 2 Promoter.
DR PRO; PR:Q9P6R0; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0000126; C:transcription factor TFIIIB complex; IDA:PomBase.
DR GO; GO:0097550; C:transcription preinitiation complex; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000995; F:RNA polymerase III general transcription initiation factor activity; IMP:PomBase.
DR GO; GO:0001006; F:RNA polymerase III type 3 promoter sequence-specific DNA binding; IDA:PomBase.
DR GO; GO:0017025; F:TBP-class protein binding; IEA:InterPro.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IBA:GO_Central.
DR GO; GO:0045945; P:positive regulation of transcription by RNA polymerase III; IC:GOC-OWL.
DR GO; GO:0070898; P:RNA polymerase III preinitiation complex assembly; ISO:PomBase.
DR GO; GO:0006383; P:transcription by RNA polymerase III; IBA:GO_Central.
DR GO; GO:0006384; P:transcription initiation from RNA polymerase III promoter; IMP:PomBase.
DR CDD; cd00043; CYCLIN; 2.
DR InterPro; IPR029529; Brf1.
DR InterPro; IPR011665; BRF1_TBP-bd_dom.
DR InterPro; IPR013763; Cyclin-like.
DR InterPro; IPR036915; Cyclin-like_sf.
DR InterPro; IPR000812; TFIIB.
DR InterPro; IPR013150; TFIIB_cyclin.
DR InterPro; IPR013137; Znf_TFIIB.
DR PANTHER; PTHR11618; PTHR11618; 1.
DR PANTHER; PTHR11618:SF4; PTHR11618:SF4; 1.
DR Pfam; PF07741; BRF1; 1.
DR Pfam; PF08271; TF_Zn_Ribbon; 1.
DR Pfam; PF00382; TFIIB; 2.
DR PRINTS; PR00685; TIFACTORIIB.
DR SMART; SM00385; CYCLIN; 2.
DR SUPFAM; SSF47954; SSF47954; 2.
DR PROSITE; PS51134; ZF_TFIIB; 1.
PE 1: Evidence at protein level;
KW Activator; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..492
FT /note="Transcription factor IIIB 60 kDa subunit"
FT /id="PRO_0000119345"
FT ZN_FING 1..30
FT /note="TFIIB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT REGION 440..468
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 3
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 6
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 22
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
FT BINDING 25
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
SQ SEQUENCE 492 AA; 55795 MW; 34B1874ECBB05D3E CRC64;
MGCPNCGSTT FESDTASGNT YCTQCGVVVE QDAIVSEVTF GEASTGAAVV QGSLVSNDQT
HARTFGGPYR NQGSVESREL TIANGRRRIS ALAIALKLNE RHIEAAVRYF TLAINNNFIK
GRRSQYVVAS CLYIVCRISK TSHMLIDFSD ILQINVFKLG STFLKLCRVL RPNLPLLDPS
LYISRFASLL EFGPETHRVA NDAIRLVARM NRDWMQIGRR PAGICGACLL IAARMNNFRR
SVREVVHVVK VADITIQKRL DEFKLTESGD LSIADFRNIW LEGQSDPPSF TKNQKFQQYG
AQKVSNIDHT QEYMSPIKRT PDFDGNEVKS EELSQTVKVE SQETPVHLKA DEREIRKEVT
ETLKGDELRK ISLQVNVKFS EEEVTLEDVD DDEIEDILLD KDEILTKTQV WMELNKDYLA
EEEAKNLKLQ EDLKKGIVRQ PRKRRRYRPR DSTSDGIADT AAESAKEMMQ QRAFSKKINY
EALDMLFDEE QS
