TF3C1_MOUSE
ID TF3C1_MOUSE Reviewed; 2101 AA.
AC Q8K284; Q8CAL9;
DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2004, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=General transcription factor 3C polypeptide 1;
DE AltName: Full=TF3C-alpha;
DE AltName: Full=TFIIIC box B-binding subunit;
DE AltName: Full=Transcription factor IIIC 220 kDa subunit;
DE Short=TFIIIC 220 kDa subunit;
DE Short=TFIIIC220;
DE AltName: Full=Transcription factor IIIC subunit alpha;
GN Name=Gtf3c1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=C57BL/6J; TISSUE=Hypothalamus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1890, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1196 AND SER-1890, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Required for RNA polymerase III-mediated transcription.
CC Component of TFIIIC that initiates transcription complex assembly on
CC tRNA and is required for transcription of 5S rRNA and other stable
CC nuclear and cytoplasmic RNAs. Binds to the box B promoter element (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Part of the TFIIIC subcomplex TFIIIC2, consisting of six
CC subunits, GTF3C1, GTF3C2, GTF3C3, GTF3C4, GTF3C5 and GTF3C6. Interacts
CC with IGHMBP2. Interacts with MAF1. {ECO:0000250|UniProtKB:Q12789}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8K284-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8K284-2; Sequence=VSP_010576;
CC Name=3;
CC IsoId=Q8K284-3; Sequence=VSP_010573, VSP_010574, VSP_010575;
CC -!- MISCELLANEOUS: [Isoform 2]: Incomplete sequence. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TFIIIC subunit 1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH32208.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK038537; BAC30032.1; -; mRNA.
DR EMBL; BC032208; AAH32208.1; ALT_INIT; mRNA.
DR EMBL; BC067041; AAH67041.1; -; mRNA.
DR CCDS; CCDS40122.1; -. [Q8K284-1]
DR RefSeq; NP_997122.1; NM_207239.1. [Q8K284-1]
DR AlphaFoldDB; Q8K284; -.
DR BioGRID; 231457; 9.
DR IntAct; Q8K284; 4.
DR MINT; Q8K284; -.
DR STRING; 10090.ENSMUSP00000056719; -.
DR iPTMnet; Q8K284; -.
DR PhosphoSitePlus; Q8K284; -.
DR SwissPalm; Q8K284; -.
DR EPD; Q8K284; -.
DR jPOST; Q8K284; -.
DR MaxQB; Q8K284; -.
DR PaxDb; Q8K284; -.
DR PeptideAtlas; Q8K284; -.
DR PRIDE; Q8K284; -.
DR ProteomicsDB; 263291; -. [Q8K284-1]
DR ProteomicsDB; 263292; -. [Q8K284-2]
DR ProteomicsDB; 263293; -. [Q8K284-3]
DR Antibodypedia; 26269; 40 antibodies from 11 providers.
DR DNASU; 233863; -.
DR Ensembl; ENSMUST00000055506; ENSMUSP00000056719; ENSMUSG00000032777. [Q8K284-1]
DR GeneID; 233863; -.
DR KEGG; mmu:233863; -.
DR UCSC; uc009jqh.1; mouse. [Q8K284-1]
DR UCSC; uc009jql.1; mouse. [Q8K284-3]
DR UCSC; uc012fto.1; mouse. [Q8K284-2]
DR CTD; 2975; -.
DR MGI; MGI:107887; Gtf3c1.
DR VEuPathDB; HostDB:ENSMUSG00000032777; -.
DR eggNOG; KOG4560; Eukaryota.
DR GeneTree; ENSGT00390000008664; -.
DR HOGENOM; CLU_001556_1_0_1; -.
DR InParanoid; Q8K284; -.
DR OMA; PHKVHVE; -.
DR OrthoDB; 143012at2759; -.
DR PhylomeDB; Q8K284; -.
DR TreeFam; TF351624; -.
DR Reactome; R-MMU-76061; RNA Polymerase III Transcription Initiation From Type 1 Promoter.
DR Reactome; R-MMU-76066; RNA Polymerase III Transcription Initiation From Type 2 Promoter.
DR BioGRID-ORCS; 233863; 25 hits in 73 CRISPR screens.
DR ChiTaRS; Gtf3c1; mouse.
DR PRO; PR:Q8K284; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q8K284; protein.
DR Bgee; ENSMUSG00000032777; Expressed in embryonic post-anal tail and 266 other tissues.
DR ExpressionAtlas; Q8K284; baseline and differential.
DR Genevisible; Q8K284; MM.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:1990904; C:ribonucleoprotein complex; IDA:MGI.
DR GO; GO:0000127; C:transcription factor TFIIIC complex; ISO:MGI.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000995; F:RNA polymerase III general transcription initiation factor activity; ISO:MGI.
DR GO; GO:0042791; P:5S class rRNA transcription by RNA polymerase III; IBA:GO_Central.
DR GO; GO:0006384; P:transcription initiation from RNA polymerase III promoter; IBA:GO_Central.
DR CDD; cd16169; Tau138_eWH; 1.
DR InterPro; IPR044210; Tfc3-like.
DR InterPro; IPR035625; Tfc3_eWH.
DR InterPro; IPR007309; TFIIIC_Bblock-bd.
DR PANTHER; PTHR15180; PTHR15180; 1.
DR Pfam; PF04182; B-block_TFIIIC; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Isopeptide bond; Nucleus;
KW Phosphoprotein; Reference proteome; Transcription; Ubl conjugation.
FT CHAIN 1..2101
FT /note="General transcription factor 3C polypeptide 1"
FT /id="PRO_0000209711"
FT REGION 473..574
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 588..609
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 718..772
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 820..864
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1186..1239
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1598..1627
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1822..1923
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 535..550
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 551..566
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 744..758
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 836..864
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1186..1201
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1203..1232
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1822..1840
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1859..1877
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 667
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q12789"
FT MOD_RES 1063
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q12789"
FT MOD_RES 1196
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1854
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q12789"
FT MOD_RES 1890
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT CROSSLNK 534
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q12789"
FT CROSSLNK 770
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q12789"
FT CROSSLNK 833
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q12789"
FT VAR_SEQ 1..156
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_010573"
FT VAR_SEQ 326..375
FT /note="TDVMVRCLKLLKEFKRKMEDDHDDDDDEEVISKGVPPVDIVFERDMLTQT
FT -> KSRTLWPTGSPCCHFLWSHSALKCEDYTSFSSLLLSAFYLKRIREVCPEF (in
FT isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_010574"
FT VAR_SEQ 376..2101
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_010575"
FT VAR_SEQ 1503..1598
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_010576"
FT CONFLICT 1599
FT /note="S -> N (in Ref. 2; AAH32208)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2101 AA; 237476 MW; 182C2EFFD9599AD6 CRC64;
MDALESLLDE VALEGLDGLC LPALWSRLES RSPAFPLPLE PYTQEFLWRA LATHPGISFY
EEPRERPDLQ LQDRYEEIDL ETGILESRRD PVTLEDVYPI HMILENKDGI QGSCRYFKER
KDITSSIRSK CLQPRCTMVE AFSRWGKKLI IVASQDMRYR ALIGLEGDPD LKLPDFSYCI
LERLGRSRWQ GELQRDLHTT AFKVDAGKLH YHRKILNKNG LITMQSHVIR LPTGAQQHSI
LLLLNRFHVD RRSKYDILME KLSMMLSTRS NQIETLGKLR EELGLCERTF KRLYQYMLNA
GLAKVVSLPL QEIHPECGPC KTKKGTDVMV RCLKLLKEFK RKMEDDHDDD DDEEVISKGV
PPVDIVFERD MLTQTYELIE RRGTKGISQA EIRVAMNVGK LEARMLCRLL QRFKVVKGFM
EDEGRQRTTK YISCVFAEES DLSRQYAREK ARGELLTTVS LASVQDESLM PEGEEAFLSD
SESEEESSCS GGKRRGRGSR GHARASGDAG SGSRPHHSTP AKGGWKVLNL HPLKKPKAAA
EERSRRSSAC RDGLDTSSSS ELNAPFDPHS MDSHSGDIAV IEEVRLDNPK EGGGSQKGGR
HGSSQDKPHK TYRLLKRRNL IIEAVTNLRL IESLFTIQKM IMDQEKQEGV STKCCKKSII
RLVRNLSEEG LLRLYRTTVI QDGIKKKVDL VVHPSMDQND PLVRSAIEQV RFRISNSSTA
NRVKVPPAPA PQEEAEEENQ EPEVPSRSAN SDPNTSSKPE STRVKKTDEK MGITPLKNYK
PVIVPGLGRS IGFLPKMPRL KAMHLFLWYL VYGHPAGHTG EQPALHSERK TGKQESSRPG
AQPSSGDDWD TSEAKNNTES SSWESEMELS TEIVYVDEIS WMRYVPPIPI HRDFGFGWAL
VSDILLCLPL SIFVQLVQVS YKVDNLEDFL NDPLKKHTLI RFLPRHIRQQ LLYKRRYIFS
VVENLQRLCY MGLLQFGPTE KFQDKDQVFV FLKKNAVIVD TTICDPHYNL AHSSRPFERR
LYVLDSMQDV ECYWFDLQCI CLNTPLGVVR CPCAQKICPD PGSDPEGSLR KEQESAMDKH
NLERKCAMLE YTTGSREVVD EGLVPGDGLG AAGLDSSFYA HLKRNWVWTS YIINKARKNN
TSENGLTGRL QTFLSKRPMP LGSGGSGRLP LWSEGRADAE LCADKEEQFE LDREPTPGRN
RKVRGGKSQK RKRLKKEPIR KTKRRRRGEH PEAKSKKLRY QDEADQNALR MMTRLRVSWS
MQEDGLLMLC RIASNVLNTK VKGPFVTWQV VRDILHATFE ESLDKTSHSV GRRARYIVKN
PQAFMNYKVC LAEVYQDKAL VGDFMSRKGN YEDPKVCAKE FKEFVEKLKE KFSSGLRNPN
LEIPNTLQEL FAKYRVLAIG DEKDRVRKED ELNSVEDIHF LVLQNLIQST LSLSNSQSNS
CQSFQIFRLY REFREPVLVR AFMECQKRSL VNRRRVSHSQ GPKKNRAVPF VPMSYQLSQS
YYKLFTWRFP TTICTESFQF YDRLRTNGML DQPDHFSFKD LDSSDPSNDL VAFSLDSPGG
HCVTALALFS LGLLSVDVRI PEQIVVVDSS MVESEVMKSL GKDGGLDDDE EEEDLDEGSG
TKRQGVEVKA HQASHTKYLL MRGYYTVPGM VSTRNLNPND SIVVNSCQVK FRLRNTPAST
QLGPTGFTAT PLEELQAGLS CLPASFTSLV DPQLRTHCPE EFAHQMAQSG YSPEDVAASL
EILQAVAAAD CFGIDKEKLS RQFSALEKIA DRRTRTFLDY IQDLLEQEQV MEVGGNTVRL
VTMASAQPWL LHSMRLRDME VDTKASGDDS QSRLPEGPSI EDHTSEGAAV PPVSSHSTKK
RPHCPETDAE EATRLPAKKP TLQDVRVAAS PRPGAEEQAE AQAPAQLAAP EDADAGGPRQ
ENQENVGVSG LEQLGCEFQL PEGSEDPRGL TESNMAQAAW ESGCERVCFV GRPWRGVDGH
LNMPVCKGML EAVLYHIMSR PGVPESCLLQ YYQGVLQPVA VLELLRGLES LGCIQKRTLR
KPASVSLFSR PVVEGLGQAS EAEALSCHES TVTFYEPTLD CTIRLGRVFP HDINWNKWIH
L
