TF3C5_HUMAN
ID TF3C5_HUMAN Reviewed; 519 AA.
AC Q9Y5Q8; A6NI44; A6NJB9; Q5T7U2; Q5T7U3; Q8N2U7; Q8N857; Q96GD9; Q9H4P2;
DT 24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2004, sequence version 2.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=General transcription factor 3C polypeptide 5;
DE AltName: Full=TF3C-epsilon;
DE AltName: Full=Transcription factor IIIC 63 kDa subunit;
DE Short=TFIIIC 63 kDa subunit;
DE Short=TFIIIC63;
DE AltName: Full=Transcription factor IIIC subunit epsilon;
GN Name=GTF3C5; ORFNames=CDABP0017;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH BRF1 AND TBP, AND
RP IDENTIFICATION OF SUBUNITS OF TFIIIC2 COMPLEX.
RX PubMed=10373544; DOI=10.1128/mcb.19.7.4944;
RA Hsieh Y.-J., Wang Z., Kovelman R., Roeder R.G.;
RT "Cloning and characterization of two evolutionarily conserved subunits
RT (TFIIIC102 and TFIIIC63) of human TFIIIC and their involvement in
RT functional interactions with TFIIIB and RNA polymerase III.";
RL Mol. Cell. Biol. 19:4944-4952(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Leukemia;
RA Zhou J., Yu W., Tang H., Mei G., Tsang Y.T.M., Bouck J., Gibbs R.A.,
RA Margolin J.F.;
RT "Pediatric leukemia cDNA sequencing project.";
RL Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain, and Spleen;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain, Kidney, Ovary, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP IDENTIFICATION IN TFIIIC COMPLEX, AND INTERACTION WITH GTF3C6.
RX PubMed=17409385; DOI=10.1074/jbc.m611542200;
RA Dumay-Odelot H., Marck C., Durrieu-Gaillard S., Lefebvre O., Jourdain S.,
RA Prochazkova M., Pflieger A., Teichmann M.;
RT "Identification, molecular cloning, and characterization of the sixth
RT subunit of human transcription factor TFIIIC.";
RL J. Biol. Chem. 282:17179-17189(2007).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [10]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Involved in RNA polymerase III-mediated transcription.
CC Integral, tightly associated component of the DNA-binding TFIIIC2
CC subcomplex that directly binds tRNA and virus-associated RNA promoters.
CC -!- SUBUNIT: Part of the TFIIIC subcomplex TFIIIC2, consisting of six
CC subunits, GTF3C1, GTF3C2, GTF3C3, GTF3C4, GTF3C5 and GTF3C6. Interacts
CC with BRF1, GTF3C6 and TBP. {ECO:0000269|PubMed:10373544,
CC ECO:0000269|PubMed:17409385}.
CC -!- INTERACTION:
CC Q9Y5Q8; Q6A162: KRT40; NbExp=3; IntAct=EBI-1045409, EBI-10171697;
CC Q9Y5Q8; P60328: KRTAP12-3; NbExp=3; IntAct=EBI-1045409, EBI-11953334;
CC Q9Y5Q8; Q99750: MDFI; NbExp=3; IntAct=EBI-1045409, EBI-724076;
CC Q9Y5Q8; Q7Z3S9: NOTCH2NLA; NbExp=3; IntAct=EBI-1045409, EBI-945833;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9Y5Q8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9Y5Q8-2; Sequence=VSP_010355;
CC Name=3;
CC IsoId=Q9Y5Q8-3; Sequence=VSP_035196;
CC -!- SIMILARITY: Belongs to the TFIIIC subunit 5 family. {ECO:0000305}.
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DR EMBL; AF133124; AAD41476.1; -; mRNA.
DR EMBL; AY007123; AAG01991.1; -; mRNA.
DR EMBL; AK055092; BAG51466.1; -; mRNA.
DR EMBL; AK097295; BAC04993.1; -; mRNA.
DR EMBL; AL162417; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471090; EAW88026.1; -; Genomic_DNA.
DR EMBL; CH471090; EAW88029.1; -; Genomic_DNA.
DR EMBL; BC009741; AAH09741.2; -; mRNA.
DR EMBL; BC011355; AAH11355.1; -; mRNA.
DR EMBL; BC017337; AAH17337.1; -; mRNA.
DR EMBL; BC030157; AAH30157.2; -; mRNA.
DR CCDS; CCDS48050.1; -. [Q9Y5Q8-3]
DR CCDS; CCDS6958.1; -. [Q9Y5Q8-1]
DR RefSeq; NP_001116295.1; NM_001122823.1. [Q9Y5Q8-3]
DR RefSeq; NP_001273638.1; NM_001286709.1.
DR RefSeq; NP_036219.2; NM_012087.3. [Q9Y5Q8-1]
DR AlphaFoldDB; Q9Y5Q8; -.
DR SMR; Q9Y5Q8; -.
DR BioGRID; 114737; 135.
DR CORUM; Q9Y5Q8; -.
DR DIP; DIP-50823N; -.
DR IntAct; Q9Y5Q8; 61.
DR MINT; Q9Y5Q8; -.
DR STRING; 9606.ENSP00000361180; -.
DR iPTMnet; Q9Y5Q8; -.
DR PhosphoSitePlus; Q9Y5Q8; -.
DR BioMuta; GTF3C5; -.
DR DMDM; 47606222; -.
DR EPD; Q9Y5Q8; -.
DR jPOST; Q9Y5Q8; -.
DR MassIVE; Q9Y5Q8; -.
DR MaxQB; Q9Y5Q8; -.
DR PaxDb; Q9Y5Q8; -.
DR PeptideAtlas; Q9Y5Q8; -.
DR PRIDE; Q9Y5Q8; -.
DR ProteomicsDB; 86475; -. [Q9Y5Q8-1]
DR ProteomicsDB; 86476; -. [Q9Y5Q8-2]
DR ProteomicsDB; 86477; -. [Q9Y5Q8-3]
DR Antibodypedia; 18242; 204 antibodies from 25 providers.
DR DNASU; 9328; -.
DR Ensembl; ENST00000372097.10; ENSP00000361169.5; ENSG00000148308.18. [Q9Y5Q8-1]
DR Ensembl; ENST00000372108.9; ENSP00000361180.5; ENSG00000148308.18. [Q9Y5Q8-3]
DR GeneID; 9328; -.
DR KEGG; hsa:9328; -.
DR MANE-Select; ENST00000372097.10; ENSP00000361169.5; NM_012087.4; NP_036219.2.
DR UCSC; uc004cci.5; human. [Q9Y5Q8-1]
DR CTD; 9328; -.
DR DisGeNET; 9328; -.
DR GeneCards; GTF3C5; -.
DR HGNC; HGNC:4668; GTF3C5.
DR HPA; ENSG00000148308; Low tissue specificity.
DR MIM; 604890; gene.
DR neXtProt; NX_Q9Y5Q8; -.
DR OpenTargets; ENSG00000148308; -.
DR PharmGKB; PA29056; -.
DR VEuPathDB; HostDB:ENSG00000148308; -.
DR eggNOG; KOG2473; Eukaryota.
DR GeneTree; ENSGT00390000004458; -.
DR HOGENOM; CLU_026463_0_0_1; -.
DR InParanoid; Q9Y5Q8; -.
DR OMA; PPEYFVR; -.
DR OrthoDB; 1111142at2759; -.
DR PhylomeDB; Q9Y5Q8; -.
DR TreeFam; TF313581; -.
DR PathwayCommons; Q9Y5Q8; -.
DR Reactome; R-HSA-749476; RNA Polymerase III Abortive And Retractive Initiation.
DR Reactome; R-HSA-76061; RNA Polymerase III Transcription Initiation From Type 1 Promoter.
DR Reactome; R-HSA-76066; RNA Polymerase III Transcription Initiation From Type 2 Promoter.
DR SignaLink; Q9Y5Q8; -.
DR SIGNOR; Q9Y5Q8; -.
DR BioGRID-ORCS; 9328; 554 hits in 1091 CRISPR screens.
DR ChiTaRS; GTF3C5; human.
DR GeneWiki; GTF3C5; -.
DR GenomeRNAi; 9328; -.
DR Pharos; Q9Y5Q8; Tdark.
DR PRO; PR:Q9Y5Q8; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; Q9Y5Q8; protein.
DR Bgee; ENSG00000148308; Expressed in right hemisphere of cerebellum and 170 other tissues.
DR ExpressionAtlas; Q9Y5Q8; baseline and differential.
DR Genevisible; Q9Y5Q8; HS.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0000127; C:transcription factor TFIIIC complex; IDA:HGNC-UCL.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000995; F:RNA polymerase III general transcription initiation factor activity; IDA:GO_Central.
DR GO; GO:0042791; P:5S class rRNA transcription by RNA polymerase III; IC:HGNC-UCL.
DR GO; GO:0035914; P:skeletal muscle cell differentiation; IEA:Ensembl.
DR GO; GO:0006383; P:transcription by RNA polymerase III; IC:HGNC-UCL.
DR GO; GO:0006384; P:transcription initiation from RNA polymerase III promoter; IEA:InterPro.
DR GO; GO:0042797; P:tRNA transcription by RNA polymerase III; IC:HGNC-UCL.
DR Gene3D; 3.30.200.160; -; 1.
DR InterPro; IPR019136; TF_IIIC_su-5_HTH.
DR InterPro; IPR040454; TF_IIIC_Tfc1/Sfc1.
DR InterPro; IPR041499; Tfc1/Sfc1_N.
DR InterPro; IPR042536; TFIIIC_tauA_Sfc1.
DR PANTHER; PTHR13230; PTHR13230; 1.
DR Pfam; PF09734; Tau95; 1.
DR Pfam; PF17682; Tau95_N; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; DNA-binding; Nucleus;
KW Reference proteome; Transcription.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:19413330,
FT ECO:0007744|PubMed:22814378"
FT CHAIN 2..519
FT /note="General transcription factor 3C polypeptide 5"
FT /id="PRO_0000209715"
FT REGION 465..519
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 483..519
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:19413330,
FT ECO:0007744|PubMed:22814378"
FT VAR_SEQ 56..65
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_010355"
FT VAR_SEQ 389
FT /note="K -> KVSLQTLR (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_035196"
FT VARIANT 445
FT /note="D -> N (in dbSNP:rs637435)"
FT /id="VAR_053727"
FT CONFLICT 372
FT /note="P -> R (in Ref. 1; AAD41476)"
FT /evidence="ECO:0000305"
FT CONFLICT 375
FT /note="T -> A (in Ref. 3; BAC04993)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 519 AA; 59571 MW; 088B8B6125CEEF36 CRC64;
MAAEAADLGL GAAVPVELRR ERRMVCVEYP GVVRDVAKML PTLGGEEGVS RIYADPTKRL
ELYFRPKDPY CHPVCANRFS TSSLLLRIRK RTRRQKGVLG TEAHSEVTFD MEILGIISTI
YKFQGMSDFQ YLAVHTEAGG KHTSMYDKVL MLRPEKEAFF HQELPLYIPP PIFSRLDAPV
DYFYRPETQH REGYNNPPIS GENLIGLSRA RRPHNAIFVN FEDEEVPKQP LEAAAQTWRR
VCTNPVDRKV EEELRKLFDI RPIWSRNAVK ANISVHPDKL KVLLPFIAYY MITGPWRSLW
IRFGYDPRKN PDAKIYQVLD FRIRCGMKHG YAPSDLPVKA KRSTYNYSLP ITVKKTSSQL
VTMHDLKQGL GPSGTSGARK PASSKYKLKD SVYIFREGAL PPYRQMFYQL CDLNVEELQK
IIHRNDGAEN SCTERDGWCL PKTSDELRDT MSLMIRQTIR SKRPALFSSS AKADGGKEQL
TYESGEDEED EEEEEEEEED FKPSDGSENE METEILDYV
