ID   TF4_BPPZA               Reviewed;         125 AA.
AC   P06952;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   23-FEB-2022, entry version 91.
DE   RecName: Full=Late genes activator p4 {ECO:0000250|UniProtKB:P03682};
DE   AltName: Full=Gene product 4 {ECO:0000250|UniProtKB:P03682};
DE            Short=gp4 {ECO:0000250|UniProtKB:P03682};
DE   AltName: Full=Protein p4 {ECO:0000250|UniProtKB:P03682};
GN   Name=4;
OS   Bacillus phage PZA (Bacteriophage PZA).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Salasmaviridae; Picovirinae; Salasvirus; Bacillus virus PZA.
OX   NCBI_TaxID=10757;
OH   NCBI_TaxID=1423; Bacillus subtilis.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3934048; DOI=10.1016/0378-1119(85)90202-1;
RA   Paces V., Vlcek C., Urbanek P., Hostomsky Z.;
RT   "Nucleotide sequence of the major early region of Bacillus subtilis phage
RT   PZA, a close relative of phi 29.";
RL   Gene 38:45-56(1985).
CC   -!- FUNCTION: Mediates, together with protein p6, the early to late
CC       transcriptional switch by stabilizing the binding of host RNA
CC       polymerase (RNAP) to the late A3 promoter. Activates transcription from
CC       the late A3 promoter and represses the main early promoters A2b and A2c
CC       by modifying the topology of the sequences encompassing early promoters
CC       A2c and A2b and late promoter A3 in a hairpin. Binds to a region of the
CC       A3 promoter located between nucleotides -50 and -100 relative to the
CC       transcription start site, that presents a sequence-directed curvature.
CC       Full induction of this curvature is needed for the transcription
CC       activation process. {ECO:0000250|UniProtKB:P03682}.
CC   -!- SUBUNIT: Interacts with host RNA polymerase (via C-terminus). Interacts
CC       with DNA; binds to the A2b, A2c and A3 promoters.
CC       {ECO:0000250|UniProtKB:P03682}.
CC   -!- SIMILARITY: Belongs to the phi29likevirus late genes activator p4
CC       family. {ECO:0000305}.
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DR   EMBL; M11813; AAA88476.1; -; Genomic_DNA.
DR   PIR; F24528; ERBP4Z.
DR   SMR; P06952; -.
DR   PRIDE; P06952; -.
DR   Proteomes; UP000000855; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:InterPro.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.70.3560; -; 1.
DR   InterPro; IPR038246; Phi-29-like_sf.
DR   InterPro; IPR008771; Phi-29_GP4.
DR   Pfam; PF05464; Phi-29_GP4; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Early protein; Repressor; Sigma factor;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..125
FT                   /note="Late genes activator p4"
FT                   /id="PRO_0000106561"
FT   DNA_BIND        77..96
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   SITE            120
FT                   /note="Interaction with host RNA polymerase and activation
FT                   of the phi29 late A3 promoter"
FT                   /evidence="ECO:0000250|UniProtKB:P03682"
SQ   SEQUENCE   125 AA;  15090 MW;  6E54063557DEE3EF CRC64;
     MPKTQRGIYH NLKESKYVAS NNDVTFFFSS ELYLNKFLDG YQEYRKKFNK KIERVAVTPW
     NMDMLADITF YSEVEKRGFH AWLKGDNATW REVHVYALRI MTKPSTLDWS RIQKPKLRER
     RKSMV