TF7L1_MOUSE
ID TF7L1_MOUSE Reviewed; 584 AA.
AC Q9Z1J1; O70450; O70573;
DT 25-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 25-MAR-2003, sequence version 2.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Transcription factor 7-like 1;
DE AltName: Full=HMG box transcription factor 3;
DE Short=TCF-3;
DE Short=mTCF-3;
GN Name=Tcf7l1; Synonyms=Tcf3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INTERACTION WITH CTNNB1.
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=9488439; DOI=10.1128/mcb.18.3.1248;
RA Korinek V., Barker N., Willert K., Molenaar M., Roose J., Wagenaar G.,
RA Markman M., Lamers W., Destree O., Clevers H.;
RT "Two members of the Tcf family implicated in Wnt/b-catenin signaling during
RT embryogenesis in the mouse.";
RL Mol. Cell. Biol. 18:1248-1256(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 28-584.
RC STRAIN=CD-1; TISSUE=Limb;
RA Melchionna R., Murphy P., La Valle R., Borello U., Cossu G.;
RT "Cloning of murine Tcf-3: a possible role in muscle development.";
RL Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 173-584.
RA Sussman D.J.;
RT "Partial mRNA sequence of mouse transcription factor mTCF-3.";
RL Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=10498690; DOI=10.1242/dev.126.20.4557;
RA DasGupta R., Fuchs E.;
RT "Multiple roles for activated LEF/TCF transcription complexes during hair
RT follicle development and differentiation.";
RL Development 126:4557-4568(1999).
RN [5]
RP FUNCTION, AND MUTAGENESIS OF LEU-383 AND PRO-407.
RX PubMed=11445543; DOI=10.1101/gad.891401;
RA Merrill B.J., Gat U., DasGupta R., Fuchs E.;
RT "Tcf3 and Lef1 regulate lineage differentiation of multipotent stem cells
RT in skin.";
RL Genes Dev. 15:1688-1705(2001).
CC -!- FUNCTION: Participates in the Wnt signaling pathway. Binds to DNA and
CC acts as a repressor in the absence of CTNNB1, and as an activator in
CC its presence. Necessary for the terminal differentiation of epidermal
CC cells, the formation of keratohyalin granules and the development of
CC the barrier function of the epidermis. {ECO:0000269|PubMed:11445543}.
CC -!- SUBUNIT: Binds the armadillo repeat of CTNNB1 and forms a stable
CC complex.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Detected in the basal layer of epidermis and in
CC outer root sheath and bulge of hair follicles.
CC {ECO:0000269|PubMed:10498690}.
CC -!- DOMAIN: The putative Groucho interaction domain between the N-terminal
CC CTNNB1 binding domain and the HMG-box is necessary for repression of
CC the transactivation mediated by TCF7L1 and CTNNB1.
CC -!- SIMILARITY: Belongs to the TCF/LEF family. {ECO:0000305}.
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DR EMBL; AJ223069; CAA11070.1; -; mRNA.
DR EMBL; AJ223233; CAA11201.1; -; mRNA.
DR EMBL; AF057691; AAC13696.1; -; mRNA.
DR RefSeq; NP_033358.2; NM_009332.3.
DR AlphaFoldDB; Q9Z1J1; -.
DR SMR; Q9Z1J1; -.
DR BioGRID; 204008; 3.
DR DIP; DIP-49605N; -.
DR IntAct; Q9Z1J1; 5.
DR STRING; 10090.ENSMUSP00000109687; -.
DR iPTMnet; Q9Z1J1; -.
DR PhosphoSitePlus; Q9Z1J1; -.
DR MaxQB; Q9Z1J1; -.
DR PaxDb; Q9Z1J1; -.
DR PRIDE; Q9Z1J1; -.
DR ProteomicsDB; 263296; -.
DR DNASU; 21415; -.
DR GeneID; 21415; -.
DR KEGG; mmu:21415; -.
DR CTD; 83439; -.
DR MGI; MGI:1202876; Tcf7l1.
DR eggNOG; KOG3248; Eukaryota.
DR InParanoid; Q9Z1J1; -.
DR Reactome; R-MMU-201722; Formation of the beta-catenin:TCF transactivating complex.
DR Reactome; R-MMU-3769402; Deactivation of the beta-catenin transactivating complex.
DR Reactome; R-MMU-4086398; Ca2+ pathway.
DR Reactome; R-MMU-4641265; Repression of WNT target genes.
DR Reactome; R-MMU-8853884; Transcriptional Regulation by VENTX.
DR Reactome; R-MMU-8951430; RUNX3 regulates WNT signaling.
DR BioGRID-ORCS; 21415; 3 hits in 74 CRISPR screens.
DR ChiTaRS; Tcf7l1; mouse.
DR PRO; PR:Q9Z1J1; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q9Z1J1; protein.
DR GO; GO:1990907; C:beta-catenin-TCF complex; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0008013; F:beta-catenin binding; IPI:MGI.
DR GO; GO:0003682; F:chromatin binding; IDA:MGI.
DR GO; GO:0003677; F:DNA binding; IDA:MGI.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0008595; P:anterior/posterior axis specification, embryo; IMP:MGI.
DR GO; GO:0048319; P:axial mesoderm morphogenesis; IMP:MGI.
DR GO; GO:0060070; P:canonical Wnt signaling pathway; IBA:GO_Central.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:1904672; P:regulation of somatic stem cell population maintenance; IMP:MGI.
DR GO; GO:2000036; P:regulation of stem cell population maintenance; IMP:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0043588; P:skin development; IGI:MGI.
DR GO; GO:0035019; P:somatic stem cell population maintenance; IGI:MGI.
DR GO; GO:0048863; P:stem cell differentiation; IMP:MGI.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IDA:MGI.
DR Gene3D; 1.10.30.10; -; 1.
DR Gene3D; 4.10.900.10; -; 1.
DR InterPro; IPR027397; Catenin-bd_sf.
DR InterPro; IPR013558; CTNNB1-bd_N.
DR InterPro; IPR009071; HMG_box_dom.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR InterPro; IPR024940; TCF/LEF.
DR InterPro; IPR028778; Tcf7l1.
DR PANTHER; PTHR10373; PTHR10373; 1.
DR PANTHER; PTHR10373:SF25; PTHR10373:SF25; 1.
DR Pfam; PF08347; CTNNB1_binding; 1.
DR Pfam; PF00505; HMG_box; 1.
DR SMART; SM00398; HMG; 1.
DR SUPFAM; SSF47095; SSF47095; 1.
DR PROSITE; PS50118; HMG_BOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation; Wnt signaling pathway.
FT CHAIN 1..584
FT /note="Transcription factor 7-like 1"
FT /id="PRO_0000048615"
FT DNA_BIND 342..410
FT /note="HMG box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT REGION 1..99
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1..71
FT /note="CTNNB1-binding"
FT /evidence="ECO:0000250"
FT REGION 159..179
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 194..231
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 412..501
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 417..423
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 76..99
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 159..173
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 215..229
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 383
FT /note="L->P: Abolishes DNA-binding and
FT transactivator/repressor activity; when associated with I-
FT 407."
FT /evidence="ECO:0000269|PubMed:11445543"
FT MUTAGEN 407
FT /note="P->I: Abolishes DNA-binding and
FT transactivator/repressor activity; when associated with P-
FT 383."
FT /evidence="ECO:0000269|PubMed:11445543"
FT CONFLICT 114
FT /note="G -> P (in Ref. 2; CAA11201)"
FT /evidence="ECO:0000305"
FT CONFLICT 301
FT /note="I -> M (in Ref. 2; CAA11201)"
FT /evidence="ECO:0000305"
FT CONFLICT 404..405
FT /note="QL -> HV (in Ref. 2; CAA11201)"
FT /evidence="ECO:0000305"
FT CONFLICT 463
FT /note="S -> I (in Ref. 2; CAA11201)"
FT /evidence="ECO:0000305"
FT CONFLICT 471
FT /note="A -> T (in Ref. 2 and 3)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 584 AA; 62298 MW; BA8B4F570AFC72CB CRC64;
MPQLGGGRGG AGGGGGGSGA GATSGGDDLG ANDELIPFQD EGGEEQEPSS DTASAQRDLD
EVKSSLVNES ENQSSSSDSE AERRPQPARD AFQKPRDYFA EVRRPQDGAF FKGGAYPGYP
FLMIPDLSSP YLSNGPLSPG GARTYLQMKW PLLDVPSSAT VKDTRSPSPA HLSNKVPVVQ
HPHHMHPLTP LITYSNDHFS PASPPTHLSP EIDPKTGIPR PPHPSELSPY YPLSPGAVGQ
IPHPLGWLVP QQGQPMYSLP PGGFRHPYPA LAMNASMSSL VSSRFPHMVA PAHPGLPTSG
IPHPAIVSPI VKQEPAAPSL SPAVSAKSPV TVKKEEEKKP HVKKPLNAFM LYMKEMRAKV
VAECTLKESA AINQILGRKW HNLSREEQAK YYELARKERQ LHAQLYPTWS ARDNYGKKKK
RKREKQLSQT QSQQQIQEAE GALASKSKKP CIQYLPPEKP CDSPASSHGS ALDSPATPSA
ALASPAAPAA THSEQAQPLS LTTKPEARAQ LALHSAAFLS AKAAASNSSQ MGSQPPLLSR
PLPLGSMPAA LLTSPPTFPA TLHAHQALPV LQAQPLSLVT KSAH
