TFB1C_ARATH
ID TFB1C_ARATH Reviewed; 579 AA.
AC Q9M322; Q5BIV2; Q67Y79;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 08-MAR-2011, sequence version 2.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=General transcription and DNA repair factor IIH subunit TFB1-3;
DE Short=AtTFB1-3 {ECO:0000303|PubMed:15645454};
DE Short=TFIIH subunit TFB1-3;
DE AltName: Full=RNA polymerase II transcription factor B subunit 1-3;
GN Name=TFB1-3 {ECO:0000305}; Synonyms=GTF2H1-2 {ECO:0000305};
GN OrderedLocusNames=At3g61420; ORFNames=F2A19.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP COMPONENT OF TFIIH CORE COMPLEX, AND NOMENCLATURE.
RX PubMed=15645454; DOI=10.1002/em.20094;
RA Kunz B.A., Anderson H.J., Osmond M.J., Vonarx E.J.;
RT "Components of nucleotide excision repair and DNA damage tolerance in
RT Arabidopsis thaliana.";
RL Environ. Mol. Mutagen. 45:115-127(2005).
CC -!- FUNCTION: Component of the general transcription and DNA repair factor
CC IIH (TFIIH) core complex, which is involved in general and
CC transcription-coupled nucleotide excision repair (NER) of damaged DNA
CC and, when complexed to CAK, in RNA transcription by RNA polymerase II.
CC In NER, TFIIH acts by opening DNA around the lesion to allow the
CC excision of the damaged oligonucleotide and its replacement by a new
CC DNA fragment. In transcription, TFIIH has an essential role in
CC transcription initiation. When the pre-initiation complex (PIC) has
CC been established, TFIIH is required for promoter opening and promoter
CC escape. Phosphorylation of the C-terminal tail (CTD) of the largest
CC subunit of RNA polymerase II by the kinase module CAK controls the
CC initiation of transcription. {ECO:0000250|UniProtKB:P32780}.
CC -!- SUBUNIT: Component of the 7-subunit TFIIH core complex composed of XPB,
CC XPD, TFB1/GTF2H1, GTF2H2/P44, TFB4/GTF2H3, TFB2/GTF2H4 and TFB5/GTF2H5,
CC which is active in NER. The core complex associates with the 3-subunit
CC CDK-activating kinase (CAK) module composed of CYCH1/cyclin H1, CDKD
CC and MAT1/At4g30820 to form the 10-subunit holoenzyme (holo-TFIIH)
CC active in transcription. {ECO:0000250|UniProtKB:P32780,
CC ECO:0000305|PubMed:15645454}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9M322-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9M322-2; Sequence=VSP_040743, VSP_040744;
CC Name=3;
CC IsoId=Q9M322-3; Sequence=VSP_040742, VSP_040745;
CC -!- SIMILARITY: Belongs to the TFB1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB71072.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL132962; CAB71072.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE80200.1; -; Genomic_DNA.
DR EMBL; AK176589; BAD44352.1; -; mRNA.
DR EMBL; BT021122; AAX22257.1; -; mRNA.
DR PIR; T47934; T47934.
DR RefSeq; NP_191701.4; NM_116007.5. [Q9M322-1]
DR AlphaFoldDB; Q9M322; -.
DR SMR; Q9M322; -.
DR STRING; 3702.AT3G61420.1; -.
DR PaxDb; Q9M322; -.
DR PRIDE; Q9M322; -.
DR ProteomicsDB; 246435; -. [Q9M322-1]
DR EnsemblPlants; AT3G61420.1; AT3G61420.1; AT3G61420. [Q9M322-1]
DR GeneID; 825315; -.
DR Gramene; AT3G61420.1; AT3G61420.1; AT3G61420. [Q9M322-1]
DR KEGG; ath:AT3G61420; -.
DR Araport; AT3G61420; -.
DR TAIR; locus:2082812; AT3G61420.
DR eggNOG; KOG2074; Eukaryota.
DR HOGENOM; CLU_017639_2_0_1; -.
DR InParanoid; Q9M322; -.
DR OMA; PHEMTEQ; -.
DR OrthoDB; 946128at2759; -.
DR PhylomeDB; Q9M322; -.
DR PRO; PR:Q9M322; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M322; baseline and differential.
DR Genevisible; Q9M322; AT.
DR GO; GO:0000439; C:transcription factor TFIIH core complex; IBA:GO_Central.
DR GO; GO:0005675; C:transcription factor TFIIH holo complex; IBA:GO_Central.
DR GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR GO; GO:0006289; P:nucleotide-excision repair; IEA:InterPro.
DR GO; GO:0070816; P:phosphorylation of RNA polymerase II C-terminal domain; IBA:GO_Central.
DR GO; GO:0006360; P:transcription by RNA polymerase I; IBA:GO_Central.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.3970.10; -; 1.
DR InterPro; IPR005607; BSD_dom.
DR InterPro; IPR035925; BSD_dom_sf.
DR InterPro; IPR027079; Tfb1/GTF2H1.
DR InterPro; IPR013876; TFIIH_BTF_p62_N.
DR PANTHER; PTHR12856; PTHR12856; 1.
DR Pfam; PF03909; BSD; 1.
DR Pfam; PF08567; PH_TFIIH; 1.
DR SMART; SM00751; BSD; 2.
DR SUPFAM; SSF140383; SSF140383; 2.
DR PROSITE; PS50858; BSD; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA damage; DNA repair; Nucleus; Reference proteome;
KW Repeat; Transcription; Transcription regulation.
FT CHAIN 1..579
FT /note="General transcription and DNA repair factor IIH
FT subunit TFB1-3"
FT /id="PRO_0000406093"
FT DOMAIN 107..161
FT /note="BSD 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00036"
FT DOMAIN 186..238
FT /note="BSD 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00036"
FT VAR_SEQ 1..335
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_040742"
FT VAR_SEQ 316..332
FT /note="RSLLQDLNRHAAVVLEG -> SIKNSHQMTSQQIVDEG (in isoform
FT 2)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_040743"
FT VAR_SEQ 333..579
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_040744"
FT VAR_SEQ 336..346
FT /note="NVQSEDTRIVA -> MYFGPLLLVLG (in isoform 3)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_040745"
SQ SEQUENCE 579 AA; 66386 MW; CF2071FDF0448608 CRC64;
MEKRVKYKSF VKDPGTLGSL ELSEVMLLFV PNDPKSDLKL KVQTHNIKSQ KYTKEGSNKP
PWLNLTSKQG RSHIFEFENY PDMHACRDFI TKALAKCEEE PNKLVVLTPA EQLSMAEFEL
RFKLLRENSE LQKLHKQFVE SKVLTEDEFW STRKKLLGKD SIRKSKQQMG LKSMMVSGIK
PSTDGRTNRV TFNLTSEIIF QIFAEKPAVR QAFINYVPKK MTEKDFWTKY FRAEYLYSTK
NTAVAAAEAA EDEELAVFLK PDEILAQEAR QKMRRVDPTL DMDADEGDDY THLMDHGIQR
DGTNDIIEPQ NDQLKRSLLQ DLNRHAAVVL EGRCINVQSE DTRIVAEALT RAKQVSKADG
EITKDANQER LERMSRATEM EDLQAPQNFP LAPLSIKDPR DYFESQQGNI LSEPRGAKAS
KRNVHEAYGL LKESILVIRM TGLSDPLIKP EVSFEVFSSL TRTISTAKNI LGKNPQESFL
DRLPKSTKDE VIHHWTSIQE LVRHFWSSYP ITTTYLSTKV GKLKDAMSNT YSLLDAMKQS
VQSDLRHQVS LLVRPMQQAL DAAFQHYESD LQRRTAKIT
