TFB1M_BOVIN
ID TFB1M_BOVIN Reviewed; 341 AA.
AC Q2TBQ0;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Mitochondrial dimethyladenosine transferase 1;
DE EC=2.1.1.-;
DE AltName: Full=Mitochondrial 12S rRNA dimethylase 1;
DE AltName: Full=Mitochondrial transcription factor B1;
DE Short=mtTFB1;
DE AltName: Full=S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 1;
DE Flags: Precursor;
GN Name=TFB1M;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=Crossbred X Angus; TISSUE=Liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase which
CC specifically dimethylates mitochondrial 12S rRNA at the conserved stem
CC loop. Also required for basal transcription of mitochondrial DNA,
CC probably via its interaction with POLRMT and TFAM. Stimulates
CC transcription independently of the methyltransferase activity (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with mitochondrial RNA polymerase POLRMT. Interacts
CC with TFAM (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q2TBQ0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q2TBQ0-2; Sequence=VSP_022489, VSP_022490;
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. rRNA adenine N(6)-methyltransferase family. KsgA
CC subfamily. {ECO:0000305}.
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DR EMBL; BC109841; AAI09842.1; -; mRNA.
DR EMBL; DT811342; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_001070364.1; NM_001076896.2. [Q2TBQ0-2]
DR RefSeq; NP_001108082.1; NM_001114610.1. [Q2TBQ0-1]
DR AlphaFoldDB; Q2TBQ0; -.
DR SMR; Q2TBQ0; -.
DR STRING; 9913.ENSBTAP00000042644; -.
DR PaxDb; Q2TBQ0; -.
DR PRIDE; Q2TBQ0; -.
DR Ensembl; ENSBTAT00000045233; ENSBTAP00000042642; ENSBTAG00000031895. [Q2TBQ0-2]
DR Ensembl; ENSBTAT00000045235; ENSBTAP00000042644; ENSBTAG00000031895. [Q2TBQ0-1]
DR GeneID; 533438; -.
DR KEGG; bta:533438; -.
DR CTD; 51106; -.
DR VEuPathDB; HostDB:ENSBTAG00000031895; -.
DR eggNOG; KOG0821; Eukaryota.
DR GeneTree; ENSGT00950000183142; -.
DR HOGENOM; CLU_041220_7_0_1; -.
DR InParanoid; Q2TBQ0; -.
DR OMA; RIEQPFK; -.
DR OrthoDB; 628543at2759; -.
DR TreeFam; TF300798; -.
DR Proteomes; UP000009136; Chromosome 9.
DR Bgee; ENSBTAG00000031895; Expressed in oocyte and 105 other tissues.
DR GO; GO:0005759; C:mitochondrial matrix; IBA:GO_Central.
DR GO; GO:0042645; C:mitochondrial nucleoid; IEA:Ensembl.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0034246; F:mitochondrial transcription factor activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000179; F:rRNA (adenine-N6,N6-)-dimethyltransferase activity; IBA:GO_Central.
DR GO; GO:0031167; P:rRNA methylation; IBA:GO_Central.
DR GO; GO:0006391; P:transcription initiation from mitochondrial promoter; IBA:GO_Central.
DR Gene3D; 1.10.8.100; -; 1.
DR Gene3D; 3.40.50.150; -; 1.
DR HAMAP; MF_00607; 16SrRNA_methyltr_A; 1.
DR InterPro; IPR001737; KsgA/Erm.
DR InterPro; IPR023165; rRNA_Ade_diMease-like_C.
DR InterPro; IPR020596; rRNA_Ade_Mease_Trfase_CS.
DR InterPro; IPR020598; rRNA_Ade_methylase_Trfase_N.
DR InterPro; IPR011530; rRNA_adenine_dimethylase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR11727; PTHR11727; 1.
DR Pfam; PF00398; RrnaAD; 1.
DR SMART; SM00650; rADc; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR TIGRFAMs; TIGR00755; ksgA; 1.
DR PROSITE; PS01131; RRNA_A_DIMETH; 1.
DR PROSITE; PS51689; SAM_RNA_A_N6_MT; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Methyltransferase; Mitochondrion;
KW Reference proteome; RNA-binding; rRNA processing; S-adenosyl-L-methionine;
KW Transcription; Transcription regulation; Transferase; Transit peptide.
FT TRANSIT 1..27
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 28..341
FT /note="Mitochondrial dimethyladenosine transferase 1"
FT /id="PRO_0000273170"
FT BINDING 35..38
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250"
FT BINDING 36
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250"
FT BINDING 38
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250"
FT BINDING 63
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250"
FT BINDING 85
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250"
FT BINDING 111
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250"
FT BINDING 141
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250"
FT VAR_SEQ 223..226
FT /note="VDSG -> NVIP (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_022489"
FT VAR_SEQ 227..341
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_022490"
SQ SEQUENCE 341 AA; 38883 MW; 978780B2A36AF2C1 CRC64;
MASSRTLGTF RLPPLPTIRE IIKLFRLQAV KQLSQNFLLD LRLTDKIVRK AGNLTNAYVY
EVGPGPGGIT RSILNAGVAE LLVVEKDSRF IPGLQMLSDA APGKLRIVHG DVLTFKIERA
FPESLKRQWE DDPPNVHIIG NLPFSVSTPL IIKWLENVSQ RNGPFAYGRT RMMLTFQKEV
AERLTATTGS KQRSRLSIMA QYLCDVQHIL TIPGQAFVPK PEVDSGVVHF TPLTRPRIKQ
PFKLVEKVVQ NAFQFRRKYC HRGLGMLFPE ARRLESTGKL LELADVDPTL RPTQLTVSHF
KSLCDVYRKM CDEDPHLFAY NFREELRQKS KKEDDKQSCR L
