ID   TFB2_ASHGO              Reviewed;         514 AA.
AC   Q75B51;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=General transcription and DNA repair factor IIH subunit TFB2;
DE            Short=TFIIH subunit TFB2;
DE   AltName: Full=RNA polymerase II transcription factor B subunit 2;
GN   Name=TFB2; OrderedLocusNames=ADL274W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Component of the general transcription and DNA repair factor
CC       IIH (TFIIH) core complex, which is involved in general and
CC       transcription-coupled nucleotide excision repair (NER) of damaged DNA
CC       and, when complexed to TFIIK, in RNA transcription by RNA polymerase
CC       II. In NER, TFIIH acts by opening DNA around the lesion to allow the
CC       excision of the damaged oligonucleotide and its replacement by a new
CC       DNA fragment. In transcription, TFIIH has an essential role in
CC       transcription initiation. When the pre-initiation complex (PIC) has
CC       been established, TFIIH is required for promoter opening and promoter
CC       escape. Phosphorylation of the C-terminal tail (CTD) of the largest
CC       subunit of RNA polymerase II by the kinase module TFIIK controls the
CC       initiation of transcription. {ECO:0000250|UniProtKB:Q02939}.
CC   -!- SUBUNIT: Component of the 7-subunit TFIIH core complex composed of
CC       XPB/SSL2, XPD/RAD3, SSL1, TFB1, TFB2, TFB4 and TFB5, which is active in
CC       NER. The core complex associates with the 3-subunit CTD-kinase module
CC       TFIIK composed of CCL1, KIN28 and TFB3 to form the 10-subunit
CC       holoenzyme (holo-TFIIH) active in transcription.
CC       {ECO:0000250|UniProtKB:Q02939}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TFB2 family. {ECO:0000305}.
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DR   EMBL; AE016817; AAS51646.1; -; Genomic_DNA.
DR   RefSeq; NP_983822.1; NM_209175.1.
DR   AlphaFoldDB; Q75B51; -.
DR   SMR; Q75B51; -.
DR   STRING; 33169.AAS51646; -.
DR   EnsemblFungi; AAS51646; AAS51646; AGOS_ADL274W.
DR   GeneID; 4619957; -.
DR   KEGG; ago:AGOS_ADL274W; -.
DR   eggNOG; KOG3471; Eukaryota.
DR   HOGENOM; CLU_027280_4_0_1; -.
DR   InParanoid; Q75B51; -.
DR   OMA; LWENERN; -.
DR   Proteomes; UP000000591; Chromosome IV.
DR   GO; GO:0000112; C:nucleotide-excision repair factor 3 complex; IEA:EnsemblFungi.
DR   GO; GO:0000439; C:transcription factor TFIIH core complex; IBA:GO_Central.
DR   GO; GO:0005675; C:transcription factor TFIIH holo complex; IBA:GO_Central.
DR   GO; GO:0001671; F:ATPase activator activity; IEA:InterPro.
DR   GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR   GO; GO:0006289; P:nucleotide-excision repair; IBA:GO_Central.
DR   GO; GO:0070816; P:phosphorylation of RNA polymerase II C-terminal domain; IBA:GO_Central.
DR   GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   InterPro; IPR040662; Tfb2_C.
DR   InterPro; IPR004598; TFIIH_p52/Tfb2.
DR   PANTHER; PTHR13152; PTHR13152; 1.
DR   Pfam; PF03849; Tfb2; 1.
DR   Pfam; PF18307; Tfb2_C; 1.
DR   TIGRFAMs; TIGR00625; tfb2; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..514
FT                   /note="General transcription and DNA repair factor IIH
FT                   subunit TFB2"
FT                   /id="PRO_0000119262"
SQ   SEQUENCE   514 AA;  58344 MW;  77492892A96797EA CRC64;
     MEPTPGIIHD GHNLFKNTVN EYLQELPQPV QSRLYQSPAT CLAIYRLLSP MAKFFIMSMI
     FQDEEVSLRD LDRWVKPDAK FQLHDAIKSM KLLHLITEGR SGQPLMVQLN SIFKESFKNA
     LTGGEVKNSF GNVVEEENDP VTMAMLDSYA ADKWETILHF MVGTPLTKSP GKNVLSLLRH
     SKLMEVDESS KELKITNEGF QFLLQDANAQ IWTLLLQYLT MAETFQMDPV DVLNLIFMIG
     ALELGKAYSV VGLSETQKTM LQDLRDYGLV FQKQSNLSKF YPTRLATMLT SDVVSIRSAS
     GAVNSVLRQR AEGVDGKVLN GTALGDDDLQ AGGEGALDGA LIVETNFKLY SYSNSPLQIA
     ILSLFIHLKT RFQNMVTGQI TRESIRRALH NGITADQIIA YMETHAHPQM RRLAGDNLEK
     KLELDPNCRD TLQVLPPTVV DQIKLWQLEL DRIISYDGYL FRDFDNLQEY QVLAQYARDI
     GVLLWSDDKK KMFFVSKEGN AQVIDFHKRK FRKK