ID   TFB2_KLULA              Reviewed;         496 AA.
AC   Q6CLR2;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=General transcription and DNA repair factor IIH subunit TFB2;
DE            Short=TFIIH subunit TFB2;
DE   AltName: Full=RNA polymerase II transcription factor B subunit 2;
GN   Name=TFB2; OrderedLocusNames=KLLA0F01056g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the general transcription and DNA repair factor
CC       IIH (TFIIH) core complex, which is involved in general and
CC       transcription-coupled nucleotide excision repair (NER) of damaged DNA
CC       and, when complexed to TFIIK, in RNA transcription by RNA polymerase
CC       II. In NER, TFIIH acts by opening DNA around the lesion to allow the
CC       excision of the damaged oligonucleotide and its replacement by a new
CC       DNA fragment. In transcription, TFIIH has an essential role in
CC       transcription initiation. When the pre-initiation complex (PIC) has
CC       been established, TFIIH is required for promoter opening and promoter
CC       escape. Phosphorylation of the C-terminal tail (CTD) of the largest
CC       subunit of RNA polymerase II by the kinase module TFIIK controls the
CC       initiation of transcription. {ECO:0000250|UniProtKB:Q02939}.
CC   -!- SUBUNIT: Component of the 7-subunit TFIIH core complex composed of
CC       XPB/SSL2, XPD/RAD3, SSL1, TFB1, TFB2, TFB4 and TFB5, which is active in
CC       NER. The core complex associates with the 3-subunit CTD-kinase module
CC       TFIIK composed of CCL1, KIN28 and TFB3 to form the 10-subunit
CC       holoenzyme (holo-TFIIH) active in transcription.
CC       {ECO:0000250|UniProtKB:Q02939}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TFB2 family. {ECO:0000305}.
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DR   EMBL; CR382126; CAG97834.1; -; Genomic_DNA.
DR   RefSeq; XP_455127.1; XM_455127.1.
DR   AlphaFoldDB; Q6CLR2; -.
DR   SMR; Q6CLR2; -.
DR   STRING; 28985.XP_455127.1; -.
DR   EnsemblFungi; CAG97834; CAG97834; KLLA0_F01056g.
DR   GeneID; 2895859; -.
DR   KEGG; kla:KLLA0_F01056g; -.
DR   eggNOG; KOG3471; Eukaryota.
DR   HOGENOM; CLU_027280_4_0_1; -.
DR   InParanoid; Q6CLR2; -.
DR   OMA; LWENERN; -.
DR   Proteomes; UP000000598; Chromosome F.
DR   GO; GO:0000112; C:nucleotide-excision repair factor 3 complex; IEA:EnsemblFungi.
DR   GO; GO:0000439; C:transcription factor TFIIH core complex; IEA:EnsemblFungi.
DR   GO; GO:0005675; C:transcription factor TFIIH holo complex; IEA:EnsemblFungi.
DR   GO; GO:0001671; F:ATPase activator activity; IEA:InterPro.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:EnsemblFungi.
DR   GO; GO:0006289; P:nucleotide-excision repair; IEA:EnsemblFungi.
DR   GO; GO:0070816; P:phosphorylation of RNA polymerase II C-terminal domain; IEA:EnsemblFungi.
DR   GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   InterPro; IPR040662; Tfb2_C.
DR   InterPro; IPR004598; TFIIH_p52/Tfb2.
DR   PANTHER; PTHR13152; PTHR13152; 1.
DR   Pfam; PF03849; Tfb2; 1.
DR   Pfam; PF18307; Tfb2_C; 1.
DR   TIGRFAMs; TIGR00625; tfb2; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..496
FT                   /note="General transcription and DNA repair factor IIH
FT                   subunit TFB2"
FT                   /id="PRO_0000119265"
SQ   SEQUENCE   496 AA;  56741 MW;  BD6DD3AC04077E6A CRC64;
     MSLNLFKTTV NDYLEGLPEQ VQSRLYESPA TCLAIYRLLS PMAKFFIMSM LFQDHDVSLR
     DLDKWVKPDA KYQLQYSIKS MKSLNLIIEG ESKQPLLIRL NPIFKKSFKN VLTGGEINNS
     FGDVADDDTN PVSTATLDQY SAEKWETILH YMVGTPNTNT PGGKVLDLLQ HSGLMEEAEY
     GELKITNQGF QFLLQDVNAQ MWTLLLQYLK MAESLQMDPV DVLNFIFMLG ALQLGKAYKC
     DQLSNTQRTM LQDMRDYGLI YQNQSDYAKF YPTRLATLLT SDTKAFRSAS VALDSVLNKA
     NETTAVEGDS GQDETTERTQ DGALIIETNF KLYSYSNSPL QIAILSLFVH LKSRFANMVT
     GQLTRESVRN ALLNGITAEQ IIAYLETHAH PRMRRLAEEN LSKKLELDPT VKETLQVLPP
     TVVDQIRLWQ LELDRIISYD GYLYTDFESY QEYQTVADYA KDIGVLLWQN EKKKMFFVST
     EGNSQVLDFH RRNFDK