TFB4_ARATH
ID TFB4_ARATH Reviewed; 301 AA.
AC Q8LF41; Q9LPQ2;
DT 17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=General transcription and DNA repair factor IIH subunit TFB4 {ECO:0000305};
DE Short=AtTFB4 {ECO:0000303|PubMed:15645454};
DE Short=TFIIH subunit TFB4 {ECO:0000305};
DE AltName: Full=RNA polymerase II transcription factor B subunit 4 {ECO:0000305};
GN Name=TFB4 {ECO:0000305}; Synonyms=GTF2H3 {ECO:0000305};
GN OrderedLocusNames=At1g18340 {ECO:0000312|Araport:AT1G18340};
GN ORFNames=F15H18.15 {ECO:0000312|EMBL:AAF25986.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP COMPONENT OF TFIIH CORE COMPLEX, AND NOMENCLATURE.
RX PubMed=15645454; DOI=10.1002/em.20094;
RA Kunz B.A., Anderson H.J., Osmond M.J., Vonarx E.J.;
RT "Components of nucleotide excision repair and DNA damage tolerance in
RT Arabidopsis thaliana.";
RL Environ. Mol. Mutagen. 45:115-127(2005).
CC -!- FUNCTION: Component of the general transcription and DNA repair factor
CC IIH (TFIIH) core complex, which is involved in general and
CC transcription-coupled nucleotide excision repair (NER) of damaged DNA
CC and, when complexed to CAK, in RNA transcription by RNA polymerase II.
CC In NER, TFIIH acts by opening DNA around the lesion to allow the
CC excision of the damaged oligonucleotide and its replacement by a new
CC DNA fragment. In transcription, TFIIH has an essential role in
CC transcription initiation. When the pre-initiation complex (PIC) has
CC been established, TFIIH is required for promoter opening and promoter
CC escape. Phosphorylation of the C-terminal tail (CTD) of the largest
CC subunit of RNA polymerase II by the kinase module CAK controls the
CC initiation of transcription. {ECO:0000250|UniProtKB:Q13889}.
CC -!- SUBUNIT: Component of the 7-subunit TFIIH core complex composed of XPB,
CC XPD, TFB1/GTF2H1, GTF2H2/P44, TFB4/GTF2H3, TFB2/GTF2H4 and TFB5/GTF2H5,
CC which is active in NER. The core complex associates with the 3-subunit
CC CDK-activating kinase (CAK) module composed of CYCH1/cyclin H1, CDKD
CC and MAT1/At4g30820 to form the 10-subunit holoenzyme (holo-TFIIH)
CC active in transcription. {ECO:0000250|UniProtKB:Q13889,
CC ECO:0000305|PubMed:15645454}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TFB4 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF25986.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC013354; AAF25986.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE29705.1; -; Genomic_DNA.
DR EMBL; BT025174; ABE77412.1; -; mRNA.
DR EMBL; AY085062; AAM61618.1; -; mRNA.
DR PIR; G86317; G86317.
DR RefSeq; NP_564050.1; NM_101692.2.
DR AlphaFoldDB; Q8LF41; -.
DR SMR; Q8LF41; -.
DR IntAct; Q8LF41; 1.
DR STRING; 3702.AT1G18340.1; -.
DR iPTMnet; Q8LF41; -.
DR PaxDb; Q8LF41; -.
DR PRIDE; Q8LF41; -.
DR ProteomicsDB; 246473; -.
DR EnsemblPlants; AT1G18340.1; AT1G18340.1; AT1G18340.
DR GeneID; 838415; -.
DR Gramene; AT1G18340.1; AT1G18340.1; AT1G18340.
DR KEGG; ath:AT1G18340; -.
DR Araport; AT1G18340; -.
DR TAIR; locus:2014124; AT1G18340.
DR eggNOG; KOG2487; Eukaryota.
DR HOGENOM; CLU_040211_1_0_1; -.
DR InParanoid; Q8LF41; -.
DR OMA; CFCHRKV; -.
DR OrthoDB; 1220881at2759; -.
DR PhylomeDB; Q8LF41; -.
DR PRO; PR:Q8LF41; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8LF41; baseline and differential.
DR GO; GO:0000439; C:transcription factor TFIIH core complex; IBA:GO_Central.
DR GO; GO:0005675; C:transcription factor TFIIH holo complex; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006289; P:nucleotide-excision repair; IBA:GO_Central.
DR GO; GO:0070816; P:phosphorylation of RNA polymerase II C-terminal domain; IBA:GO_Central.
DR Gene3D; 3.40.50.410; -; 1.
DR InterPro; IPR004600; TFIIH_Tfb4/GTF2H3.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR12831; PTHR12831; 1.
DR Pfam; PF03850; Tfb4; 1.
PE 2: Evidence at transcript level;
KW DNA damage; DNA repair; Metal-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..301
FT /note="General transcription and DNA repair factor IIH
FT subunit TFB4"
FT /id="PRO_0000435436"
FT ZN_FING 259..276
FT /note="C4-type"
FT /evidence="ECO:0000305"
SQ SEQUENCE 301 AA; 33068 MW; 385E455C709C95F4 CRC64;
MPAIASKQYS DDVSLLVLLL DTNPLFWSTT SITFSQFLSH VLAFLNAVLG LNQLNQVVVI
ATGYSSCDYI YDSSLTSNHG NFESNGTGMP AIFGSLLKKL EEFVTKDEEL SKEEVSEDRI
PSCLLSGSLS MALCYIQRVF RSGHLHPQPR ILCLQGSPDG PEQYVAVMNS IFSAQRLMVP
IDSCYIGVQN SAFLQQASYI TGGVHHTPKQ LDGLFQYLTT IFATDLHSRG FVQLPKPIGV
DFRASCFCHK KTIDMGYICS VCLSIFCEHH KKCSTCGSVF GQSKLDDASS ASDKKRKAPS
T
