ID   TFB4_ASHGO              Reviewed;         341 AA.
AC   Q75B93;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=General transcription and DNA repair factor IIH subunit TFB4;
DE            Short=TFIIH subunit TFB4;
DE   AltName: Full=RNA polymerase II transcription factor B subunit 4;
GN   Name=TFB4; OrderedLocusNames=ADL323C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Component of the general transcription and DNA repair factor
CC       IIH (TFIIH) core complex, which is involved in general and
CC       transcription-coupled nucleotide excision repair (NER) of damaged DNA
CC       and, when complexed to TFIIK, in RNA transcription by RNA polymerase
CC       II. In NER, TFIIH acts by opening DNA around the lesion to allow the
CC       excision of the damaged oligonucleotide and its replacement by a new
CC       DNA fragment. In transcription, TFIIH has an essential role in
CC       transcription initiation. When the pre-initiation complex (PIC) has
CC       been established, TFIIH is required for promoter opening and promoter
CC       escape. Phosphorylation of the C-terminal tail (CTD) of the largest
CC       subunit of RNA polymerase II by the kinase module TFIIK controls the
CC       initiation of transcription. {ECO:0000250|UniProtKB:Q12004}.
CC   -!- SUBUNIT: Component of the 7-subunit TFIIH core complex composed of
CC       XPB/SSL2, XPD/RAD3, SSL1, TFB1, TFB2, TFB4 and TFB5, which is active in
CC       NER. The core complex associates with the 3-subunit CTD-kinase module
CC       TFIIK composed of CCL1, KIN28 and TFB3 to form the 10-subunit
CC       holoenzyme (holo-TFIIH) active in transcription.
CC       {ECO:0000250|UniProtKB:Q12004}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TFB4 family. {ECO:0000305}.
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DR   EMBL; AE016817; AAS51597.1; -; Genomic_DNA.
DR   RefSeq; NP_983773.1; NM_209126.1.
DR   AlphaFoldDB; Q75B93; -.
DR   SMR; Q75B93; -.
DR   STRING; 33169.AAS51597; -.
DR   EnsemblFungi; AAS51597; AAS51597; AGOS_ADL323C.
DR   GeneID; 4619908; -.
DR   KEGG; ago:AGOS_ADL323C; -.
DR   eggNOG; KOG2487; Eukaryota.
DR   HOGENOM; CLU_040211_0_0_1; -.
DR   InParanoid; Q75B93; -.
DR   OMA; CFCHRKV; -.
DR   Proteomes; UP000000591; Chromosome IV.
DR   GO; GO:0000112; C:nucleotide-excision repair factor 3 complex; IEA:EnsemblFungi.
DR   GO; GO:0000439; C:transcription factor TFIIH core complex; IBA:GO_Central.
DR   GO; GO:0005675; C:transcription factor TFIIH holo complex; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006289; P:nucleotide-excision repair; IBA:GO_Central.
DR   GO; GO:0070816; P:phosphorylation of RNA polymerase II C-terminal domain; IBA:GO_Central.
DR   GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   Gene3D; 3.40.50.410; -; 1.
DR   InterPro; IPR004600; TFIIH_Tfb4/GTF2H3.
DR   InterPro; IPR036465; vWFA_dom_sf.
DR   PANTHER; PTHR12831; PTHR12831; 1.
DR   Pfam; PF03850; Tfb4; 1.
DR   TIGRFAMs; TIGR00627; tfb4; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; Metal-binding; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..341
FT                   /note="General transcription and DNA repair factor IIH
FT                   subunit TFB4"
FT                   /id="PRO_0000119269"
FT   ZN_FING         290..309
FT                   /note="C4-type"
SQ   SEQUENCE   341 AA;  37863 MW;  91D6646AE86395C4 CRC64;
     MDAIADSTFQ INKSKVQLVE ETPSLLTLVI DTNPKLWAEF DREVGKKGQL MQVLKSTIVF
     LNAHLSFNNS NQVSVIAACS RGIKYLYPQA DDKEGSTKKK KSEDRSIINR NMYRGFRNVD
     EAVVEELYRV FQQESKQLED GVPQPFRSTL SGAMSAGLTY INRITHETEG VSLKSRLLVI
     TCGSSASKDE VFQYIPIMNC IFSATKMKCP IDVVKVGGVK ESTFLQQATD ATNGNYLHVA
     NTDGLIQYLS TAMFIDPSLR QWVVKPNQSS VDFRTSCYLT GKVVAIGFVC SVCLCVLSII
     PPGNKCPACD SEFDEKVVAK LSRKPVITGT LRKKKKKKVT K