ID   TFB4_KLULA              Reviewed;         337 AA.
AC   Q6CVX9;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=General transcription and DNA repair factor IIH subunit TFB4;
DE            Short=TFIIH subunit TFB4;
DE   AltName: Full=RNA polymerase II transcription factor B subunit 4;
GN   Name=TFB4; OrderedLocusNames=KLLA0B08569g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the general transcription and DNA repair factor
CC       IIH (TFIIH) core complex, which is involved in general and
CC       transcription-coupled nucleotide excision repair (NER) of damaged DNA
CC       and, when complexed to TFIIK, in RNA transcription by RNA polymerase
CC       II. In NER, TFIIH acts by opening DNA around the lesion to allow the
CC       excision of the damaged oligonucleotide and its replacement by a new
CC       DNA fragment. In transcription, TFIIH has an essential role in
CC       transcription initiation. When the pre-initiation complex (PIC) has
CC       been established, TFIIH is required for promoter opening and promoter
CC       escape. Phosphorylation of the C-terminal tail (CTD) of the largest
CC       subunit of RNA polymerase II by the kinase module TFIIK controls the
CC       initiation of transcription. {ECO:0000250|UniProtKB:Q12004}.
CC   -!- SUBUNIT: Component of the 7-subunit TFIIH core complex composed of
CC       XPB/SSL2, XPD/RAD3, SSL1, TFB1, TFB2, TFB4 and TFB5, which is active in
CC       NER. The core complex associates with the 3-subunit CTD-kinase module
CC       TFIIK composed of CCL1, KIN28 and TFB3 to form the 10-subunit
CC       holoenzyme (holo-TFIIH) active in transcription.
CC       {ECO:0000250|UniProtKB:Q12004}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TFB4 family. {ECO:0000305}.
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DR   EMBL; CR382122; CAH02303.1; -; Genomic_DNA.
DR   RefSeq; XP_451910.1; XM_451910.1.
DR   AlphaFoldDB; Q6CVX9; -.
DR   SMR; Q6CVX9; -.
DR   STRING; 28985.XP_451910.1; -.
DR   EnsemblFungi; CAH02303; CAH02303; KLLA0_B08569g.
DR   GeneID; 2897346; -.
DR   KEGG; kla:KLLA0_B08569g; -.
DR   eggNOG; KOG2487; Eukaryota.
DR   HOGENOM; CLU_040211_0_0_1; -.
DR   InParanoid; Q6CVX9; -.
DR   OMA; CFCHRKV; -.
DR   Proteomes; UP000000598; Chromosome B.
DR   GO; GO:0000112; C:nucleotide-excision repair factor 3 complex; IEA:EnsemblFungi.
DR   GO; GO:0000439; C:transcription factor TFIIH core complex; IEA:EnsemblFungi.
DR   GO; GO:0005675; C:transcription factor TFIIH holo complex; IEA:EnsemblFungi.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006289; P:nucleotide-excision repair; IEA:EnsemblFungi.
DR   GO; GO:0070816; P:phosphorylation of RNA polymerase II C-terminal domain; IEA:EnsemblFungi.
DR   GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   Gene3D; 3.40.50.410; -; 1.
DR   InterPro; IPR004600; TFIIH_Tfb4/GTF2H3.
DR   InterPro; IPR036465; vWFA_dom_sf.
DR   PANTHER; PTHR12831; PTHR12831; 1.
DR   Pfam; PF03850; Tfb4; 1.
DR   SUPFAM; SSF53300; SSF53300; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; Metal-binding; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..337
FT                   /note="General transcription and DNA repair factor IIH
FT                   subunit TFB4"
FT                   /id="PRO_0000119272"
FT   ZN_FING         287..306
FT                   /note="C4-type"
SQ   SEQUENCE   337 AA;  36843 MW;  4AB9F60F43C20C3A CRC64;
     MDAIADTVFQ GTKSKTQEIE DTPSLLTVVV DTSIHSWVQL TKQQSGSGSE GSSGEKQLIE
     ALKSIVVFLN AHLAFNSGNQ VCLIAAHSEG MKYLYPSADS KPSMSMVSSD MYRGFRNVDE
     IVVEQWYRLF KEELEGQESK VSMKSSLSGA MSSALTYVNR ILKENENTSL RSRLLVITCG
     TSQGKDEIFQ YIPIMNCIFS ATKMKCSIDV VKIGGGIEST FLQQATDATS GVYLHVENTR
     GLIQYLSTAM FIDPSLRNVI IKPNQGSVDF RTSCFLTGKV VAVGFVCSVC LCVLSVIPPG
     QKCPACDSPF DSKIIARLRR KPVLSNGVPK KKNVSNK