ID   TFB4_SCHPO              Reviewed;         297 AA.
AC   O74366; O13669;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   25-MAY-2022, entry version 131.
DE   RecName: Full=General transcription and DNA repair factor IIH subunit tfb4;
DE            Short=TFIIH subunit tfb4;
DE   AltName: Full=RNA polymerase II transcription factor B subunit 4;
GN   Name=tfb4; ORFNames=pi078, SPBC30B4.07c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=10620777;
RX   DOI=10.1002/(sici)1097-0061(20000115)16:1<71::aid-yea505>3.0.co;2-5;
RA   Machida M., Yamazaki S., Kunihiro S., Tanaka T., Kushida N., Jinno K.,
RA   Haikawa Y., Yamazaki J., Yamamoto S., Sekine M., Oguchi A., Nagai Y.,
RA   Sakai M., Aoki K., Ogura K., Kudoh Y., Kikuchi H., Zhang M.Q., Yanagida M.;
RT   "A 38 kb segment containing the cdc2 gene from the left arm of fission
RT   yeast chromosome II: sequence analysis and characterization of the genomic
RT   DNA and cDNAs encoded on the segment.";
RL   Yeast 16:71-80(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [3]
RP   SUBUNIT.
RX   PubMed=14534314; DOI=10.1074/jbc.m306750200;
RA   Spaehr H., Khorosjutina O., Baraznenok V., Linder T., Samuelsen C.O.,
RA   Hermand D., Maekelae T.P., Holmberg S., Gustafsson C.M.;
RT   "Mediator influences Schizosaccharomyces pombe RNA polymerase II-dependent
RT   transcription in vitro.";
RL   J. Biol. Chem. 278:51301-51306(2003).
CC   -!- FUNCTION: Component of the general transcription and DNA repair factor
CC       IIH (TFIIH) core complex, which is involved in general and
CC       transcription-coupled nucleotide excision repair (NER) of damaged DNA
CC       and, when complexed to TFIIK, in RNA transcription by RNA polymerase
CC       II. In NER, TFIIH acts by opening DNA around the lesion to allow the
CC       excision of the damaged oligonucleotide and its replacement by a new
CC       DNA fragment. In transcription, TFIIH has an essential role in
CC       transcription initiation. When the pre-initiation complex (PIC) has
CC       been established, TFIIH is required for promoter opening and promoter
CC       escape. Phosphorylation of the C-terminal tail (CTD) of the largest
CC       subunit of RNA polymerase II by the kinase module TFIIK controls the
CC       initiation of transcription. {ECO:0000250|UniProtKB:Q12004}.
CC   -!- SUBUNIT: Component of the 7-subunit TFIIH core complex composed of
CC       XPB/ptr8, XPD/rad15, ssl1, tfb1, tfb2, tfb4 and tfb5, which is active
CC       in NER. The core complex associates with the 3-subunit CTD-kinase
CC       module TFIIK composed of mcs2/cyclin H, mcs6/cdk7 and pmh1/tfb3 to form
CC       the 10-subunit holoenzyme (holo-TFIIH) active in transcription.
CC       {ECO:0000269|PubMed:14534314}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TFB4 family. {ECO:0000305}.
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DR   EMBL; AB004539; BAA21460.1; -; Genomic_DNA.
DR   EMBL; CU329671; CAA20320.1; -; Genomic_DNA.
DR   PIR; T40173; T40173.
DR   RefSeq; NP_595532.1; NM_001021442.2.
DR   AlphaFoldDB; O74366; -.
DR   SMR; O74366; -.
DR   BioGRID; 276872; 2.
DR   IntAct; O74366; 1.
DR   STRING; 4896.SPBC30B4.07c.1; -.
DR   MaxQB; O74366; -.
DR   PaxDb; O74366; -.
DR   EnsemblFungi; SPBC30B4.07c.1; SPBC30B4.07c.1:pep; SPBC30B4.07c.
DR   GeneID; 2540343; -.
DR   KEGG; spo:SPBC30B4.07c; -.
DR   PomBase; SPBC30B4.07c; tfb4.
DR   VEuPathDB; FungiDB:SPBC30B4.07c; -.
DR   eggNOG; KOG2487; Eukaryota.
DR   HOGENOM; CLU_040211_0_0_1; -.
DR   InParanoid; O74366; -.
DR   OMA; DYRASCH; -.
DR   PhylomeDB; O74366; -.
DR   Reactome; R-SPO-113418; Formation of the Early Elongation Complex.
DR   Reactome; R-SPO-5696395; Formation of Incision Complex in GG-NER.
DR   Reactome; R-SPO-5696400; Dual Incision in GG-NER.
DR   Reactome; R-SPO-674695; RNA Polymerase II Pre-transcription Events.
DR   Reactome; R-SPO-6781823; Formation of TC-NER Pre-Incision Complex.
DR   Reactome; R-SPO-6782135; Dual incision in TC-NER.
DR   Reactome; R-SPO-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR   Reactome; R-SPO-6796648; TP53 Regulates Transcription of DNA Repair Genes.
DR   Reactome; R-SPO-72086; mRNA Capping.
DR   Reactome; R-SPO-73772; RNA Polymerase I Promoter Escape.
DR   Reactome; R-SPO-73776; RNA Polymerase II Promoter Escape.
DR   Reactome; R-SPO-73779; RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
DR   Reactome; R-SPO-75953; RNA Polymerase II Transcription Initiation.
DR   Reactome; R-SPO-75955; RNA Polymerase II Transcription Elongation.
DR   Reactome; R-SPO-76042; RNA Polymerase II Transcription Initiation And Promoter Clearance.
DR   Reactome; R-SPO-77075; RNA Pol II CTD phosphorylation and interaction with CE.
DR   PRO; PR:O74366; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0000439; C:transcription factor TFIIH core complex; IBA:GO_Central.
DR   GO; GO:0005675; C:transcription factor TFIIH holo complex; ISO:PomBase.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016251; F:RNA polymerase II general transcription initiation factor activity; ISO:PomBase.
DR   GO; GO:0006289; P:nucleotide-excision repair; ISO:PomBase.
DR   GO; GO:0070816; P:phosphorylation of RNA polymerase II C-terminal domain; IBA:GO_Central.
DR   GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; ISO:PomBase.
DR   Gene3D; 3.40.50.410; -; 1.
DR   InterPro; IPR004600; TFIIH_Tfb4/GTF2H3.
DR   InterPro; IPR036465; vWFA_dom_sf.
DR   PANTHER; PTHR12831; PTHR12831; 1.
DR   Pfam; PF03850; Tfb4; 1.
DR   TIGRFAMs; TIGR00627; tfb4; 1.
PE   1: Evidence at protein level;
KW   DNA damage; DNA repair; Metal-binding; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..297
FT                   /note="General transcription and DNA repair factor IIH
FT                   subunit tfb4"
FT                   /id="PRO_0000119273"
FT   ZN_FING         265..282
FT                   /note="C4-type"
FT   CONFLICT        288..297
FT                   /note="VEPMSKKLHT -> DQTISLRGIHK (in Ref. 1; BAA21460)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   297 AA;  33277 MW;  70A88655E58D2D02 CRC64;
     MDEFQTLKQN ASWDISEDAN DTPSLLVVIL DANPASWYSL SKKVPVSKVL ADITVFLNAH
     LAFHHDNRVA VLASHSDKVE YLYPSIAPEQ KVAEVDPTKE ANTYRKFREV DDLVLSGMKR
     LMSSTDKVSR KTMISGALSR ALAYINQVQN KNTLRSRILI FSLTGDVALQ YIPTMNCIFC
     AQKKNIPINV CNIEGGTLFL EQAADATGGI YLKVDNPKGL LQYLMMSLFP DQNLRKHLNT
     PNQANVDFRA TCFCHKKVLD IGFVCSVCLS IFCEPRVHCS TCHTKFTVEP MSKKLHT