TFDB_DELAC
ID TFDB_DELAC Reviewed; 586 AA.
AC Q8KN28; Q93T15; Q9RP01;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2004, sequence version 3.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=2,4-dichlorophenol 6-monooxygenase;
DE EC=1.14.13.20;
DE AltName: Full=2,4-dichlorophenol hydroxylase;
DE Short=2,4-DCP hydroxylase;
GN Name=tfdB;
OS Delftia acidovorans (Pseudomonas acidovorans) (Comamonas acidovorans).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Delftia.
OX NCBI_TaxID=80866 {ECO:0000312|EMBL:AAM76774.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=P4a {ECO:0000312|EMBL:AAM76774.1};
RX PubMed=12949179; DOI=10.1099/mic.0.26260-0;
RA Hoffmann D., Kleinsteuber S., Mueller R.H., Babel W.;
RT "A transposon encoding the complete 2,4-dichlorophenoxyacetic acid
RT degradation pathway in the alkalitolerant strain Delftia acidovorans P4a.";
RL Microbiology 149:2545-2556(2003).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 57-434.
RC STRAIN=P4a {ECO:0000312|EMBL:AAM76774.1};
RA Hoffmann D., Kleinsteuber S., Mueller R.H., Babel W.;
RT "Development and application of PCR primers for the detection of the tfd
RT genes in Delftia acidovorans P4a involved in the degradation of 2,4-D.";
RL Acta Biotechnol. 21:321-331(2001).
RN [3] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 59-432.
RC STRAIN=MC1 {ECO:0000312|EMBL:AAD55079.1};
RX PubMed=11572451; DOI=10.1078/0944-5013-00089;
RA Mueller R.H., Kleinsteuber S., Babel W.;
RT "Physiological and genetic characteristics of two bacterial strains
RT utilizing phenoxypropionate and phenoxyacetate herbicides.";
RL Microbiol. Res. 156:121-131(2001).
RN [4]
RP PROTEIN SEQUENCE OF 1-19.
RC STRAIN=MC1;
RX PubMed=15073309; DOI=10.1099/mic.0.26774-0;
RA Benndorf D., Davidson I., Babel W.;
RT "Regulation of catabolic enzymes during long-term exposure of Delftia
RT acidovorans MC1 to chlorophenoxy herbicides.";
RL Microbiology 150:1005-1014(2004).
CC -!- FUNCTION: Transforms 2,4-dichlorophenol (2,4-DCP) into 3,5-
CC dichlorocatechol. {ECO:0000250|UniProtKB:P27138}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2,4-dichlorophenol + H(+) + NADPH + O2 = 3,5-dichlorocatechol
CC + H2O + NADP(+); Xref=Rhea:RHEA:20920, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:15788,
CC ChEBI:CHEBI:16738, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC EC=1.14.13.20; Evidence={ECO:0000250|UniProtKB:P27138};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000250|UniProtKB:P27138};
CC -!- PATHWAY: Xenobiotic degradation; (2,4-dichlorophenoxy)acetate
CC degradation.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:P27138}.
CC -!- SIMILARITY: Belongs to the PheA/TfdB FAD monooxygenase family.
CC {ECO:0000305}.
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DR EMBL; AY078159; AAM76774.1; -; Genomic_DNA.
DR EMBL; AF176242; AAD55079.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8KN28; -.
DR SMR; Q8KN28; -.
DR UniPathway; UPA00685; -.
DR GO; GO:0018666; F:2,4-dichlorophenol 6-monooxygenase activity; IEA:UniProtKB-EC.
DR GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR GO; GO:0046300; P:2,4-dichlorophenoxyacetic acid catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR002938; FAD-bd.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR Pfam; PF01494; FAD_binding_3; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
PE 1: Evidence at protein level;
KW Aromatic hydrocarbons catabolism; Direct protein sequencing; FAD;
KW Flavoprotein; Monooxygenase; NADP; Oxidoreductase.
FT CHAIN 1..586
FT /note="2,4-dichlorophenol 6-monooxygenase"
FT /id="PRO_0000214050"
FT BINDING 11..40
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255"
FT BINDING 304..314
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255"
FT CONFLICT 2
FT /note="Missing (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 18
FT /note="G -> A (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 195
FT /note="M -> V (in Ref. 3; AAD55079)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 586 AA; 64405 MW; 6BEE58517E7B860E CRC64;
MNEKATVIET DVLVVGSGPA GAASTLLLAT YGVKTLCVSK YATTSRTPRS HITNQRTMEV
MRDLGLELEC EAMASPAELM GENVYCTSLV GDELGRVLTW GTHPQRRADY ELASPTHMCD
LPQNLLEPIM INHAARRGAD VRFHTEFVSL KQDETGVTAT VRDHLLDRQY DIRAKYLIGA
DGANSQVVDQ VGLPMEGKMG VSGSINVVFE ADLTKYVGHR PSVLYWVIQP GSSVGGLGIG
VIRMVRPWNK WLCIWGYDIA GGPPDLNEAH ARQIVHSLLG DSTIPVKIES TSTWTVNDMY
ATRLFDNRVF CMGDAVHRHP PTNGLGSNTS IQDAFNLCWK LSHVLQGKAG PELLATYNEE
RAPVARQVVQ RANKSLGDFP PILAALGLFD TKDPEQMQRN IARLKEQSPE AQEQRAALRA
AIDGTQYVYN AHGVEMNQRY QSAAIVPDGT PDPGFRRDSE LYHAHSGRPG APVPHVWVTR
HGRRVSTLDL CGKGRFSLLS GIAGSPWVEA AVHAAESLGI DLDVHIIGPG QELEDLYGDF
ARVREIEESG ALLVRPDNFI CWRAMRWQEG SGDELRAALK RVLSVH
