ID   TFDC_BURCE              Reviewed;         255 AA.
AC   P0A397; P05403; P71131;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=Chlorocatechol 1,2-dioxygenase;
DE            EC=1.13.11.-;
GN   Name=tfdC; Synonyms=tfdCI;
OS   Burkholderia cepacia (Pseudomonas cepacia).
OG   Plasmid pMAB1.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX   NCBI_TaxID=292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CSV90;
RX   PubMed=7509586; DOI=10.1128/aem.60.1.307-312.1994;
RA   Bhat M.A., Tsuda M., Nozaki M., Horiike K., Vaidyanathan C.S., Nakazawa T.;
RT   "Identification and characterization of a new plasmid carrying genes for
RT   degradation of 2,4-dichlorophenoxyacetate from Pseudomonas cepacia CSV90.";
RL   Appl. Environ. Microbiol. 60:307-312(1994).
CC   -!- FUNCTION: Preferentially converts 3,5-dichlorocatechol as opposed to
CC       other chlorinated catechols. Retains diminished activity toward non-
CC       chlorinated substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3,5-dichlorocatechol + O2 = (2E,4E)-2,4-dichloromuconate + 2
CC         H(+); Xref=Rhea:RHEA:48572, ChEBI:CHEBI:11438, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:15788;
CC   -!- COFACTOR:
CC       Name=Fe(3+); Xref=ChEBI:CHEBI:29034;
CC       Note=Binds 1 Fe(3+) ion per subunit.;
CC   -!- PATHWAY: Aromatic compound metabolism; 3-chlorocatechol degradation.
CC   -!- SIMILARITY: Belongs to the intradiol ring-cleavage dioxygenase family.
CC       {ECO:0000305}.
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DR   EMBL; D16356; BAA03859.1; -; Genomic_DNA.
DR   AlphaFoldDB; P0A397; -.
DR   SMR; P0A397; -.
DR   UniPathway; UPA00083; -.
DR   GO; GO:0018576; F:catechol 1,2-dioxygenase activity; IEA:InterPro.
DR   GO; GO:0018575; F:chlorocatechol 1,2-dioxygenase activity; IEA:InterPro.
DR   GO; GO:0008199; F:ferric iron binding; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0009712; P:catechol-containing compound metabolic process; IEA:InterPro.
DR   CDD; cd03462; 1_2-CCD; 1.
DR   Gene3D; 2.60.130.10; -; 1.
DR   InterPro; IPR007535; Catechol_dOase_N.
DR   InterPro; IPR012817; Chlorcchol_dOase.
DR   InterPro; IPR000627; Intradiol_dOase_C.
DR   InterPro; IPR015889; Intradiol_dOase_core.
DR   Pfam; PF00775; Dioxygenase_C; 1.
DR   Pfam; PF04444; Dioxygenase_N; 1.
DR   SUPFAM; SSF49482; SSF49482; 1.
DR   TIGRFAMs; TIGR02465; chlorocat_1_2; 1.
DR   PROSITE; PS00083; INTRADIOL_DIOXYGENAS; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Dioxygenase; Iron; Metal-binding;
KW   Oxidoreductase; Plasmid.
FT   CHAIN           1..255
FT                   /note="Chlorocatechol 1,2-dioxygenase"
FT                   /id="PRO_0000085091"
FT   BINDING         130
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         164
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         188
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         190
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   255 AA;  28283 MW;  5BCC1BC6A94B19B4 CRC64;
     MNKRVKDVVD AIVAAVQRVL DQKEVTEAEY RTAVHYLMQV AEQRETALLC DVFFNSTVAA
     TKARISEGST PAIEGPYYRD DAPLVDDRLK TYDTDDHKPL LIQGTVKAVD GSVVEDVTID
     VWHSTPDGKY SGFHDDIPTD FYRGKLRVGT DGSFRVRTTM PVPYQIPDQG PTGALLETMG
     GHSWRPAHVH FKVKAPGYET LTTQYYFEGG DWITDDCCNG VQSSLITPDI VEEGVRLMNI
     NFVIEPARAQ AGANP