TFDP_DROME
ID TFDP_DROME Reviewed; 445 AA.
AC Q24318; B8A3X4; O17472; O44080; Q86PE0; Q8ML56; Q9V6M0;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 21-JUN-2005, sequence version 2.
DT 03-AUG-2022, entry version 180.
DE RecName: Full=Transcription factor Dp;
GN Name=Dp; ORFNames=CG4654;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Booth B.,
RA Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E.,
RA George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G.,
RA Miranda A., Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S.,
RA Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M.,
RA Celniker S.E.;
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 50-445.
RC TISSUE=Eye imaginal disk;
RX PubMed=8022787; DOI=10.1073/pnas.91.14.6359;
RA Dynlacht B.D., Brook A., Dembski M., Yenush L., Dyson N.;
RT "DNA-binding and trans-activation properties of Drosophila E2F and DP
RT proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:6359-6363(1994).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 83-445.
RX PubMed=9271122; DOI=10.1101/gad.11.15.1999;
RA Royzman I., Whittaker A.J., Orr-Weaver T.L.;
RT "Mutations in Drosophila DP and E2F distinguish G1-S progression from an
RT associated transcriptional program.";
RL Genes Dev. 11:1999-2011(1997).
RN [7]
RP FUNCTION, AND IDENTIFICATION IN THE DREAM COMPLEX.
RX PubMed=15479636; DOI=10.1016/j.cell.2004.09.034;
RA Korenjak M., Taylor-Harding B., Binne U.K., Satterlee J.S., Stevaux O.,
RA Aasland R., White-Cooper H., Dyson N., Brehm A.;
RT "Native E2F/RBF complexes contain Myb-interacting proteins and repress
RT transcription of developmentally controlled E2F target genes.";
RL Cell 119:181-193(2004).
RN [8]
RP IDENTIFICATION IN THE DREAM COMPLEX.
RX PubMed=15545624; DOI=10.1101/gad.1255204;
RA Lewis P.W., Beall E.L., Fleischer T.C., Georlette D., Link A.J.,
RA Botchan M.R.;
RT "Identification of a Drosophila Myb-E2F2/RBF transcriptional repressor
RT complex.";
RL Genes Dev. 18:2929-2940(2004).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-398, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Component of the DREAM complex, a multiprotein complex that
CC can both act as a transcription activator or repressor depending on the
CC context. In follicle cells, the complex plays a central role in the
CC site-specific DNA replication at the chorion loci. During development,
CC the complex represses transcription of developmentally controlled E2F
CC target genes. Can stimulate E2F-dependent transcription.
CC {ECO:0000269|PubMed:15479636}.
CC -!- SUBUNIT: Heterodimer of E2f and Dp. Cooperate to give sequence-specific
CC DNA binding and optimal trans-activation. Component of the DREAM
CC complex at least composed of Myb, Caf1-55, mip40, mip120, mip130, E2f2,
CC Dp, Rbf, Rbf2, lin-52, HDAC1/Rpd3 and l(3)mbt.
CC {ECO:0000269|PubMed:15479636, ECO:0000269|PubMed:15545624}.
CC -!- INTERACTION:
CC Q24318; Q24472: Rbf; NbExp=3; IntAct=EBI-530830, EBI-145741;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A;
CC IsoId=Q24318-1; Sequence=Displayed;
CC Name=B;
CC IsoId=Q24318-2; Sequence=VSP_014146;
CC -!- SIMILARITY: Belongs to the E2F/DP family. {ECO:0000305}.
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DR EMBL; AE013599; AAF58403.1; -; Genomic_DNA.
DR EMBL; AE013599; AAM68602.1; -; Genomic_DNA.
DR EMBL; AY069526; AAL39671.1; -; mRNA.
DR EMBL; BT003183; AAO24938.1; -; mRNA.
DR EMBL; BT056266; ACL68713.1; -; mRNA.
DR EMBL; X79708; CAA56147.2; -; mRNA.
DR EMBL; AF011362; AAB87765.1; -; Genomic_DNA.
DR PIR; B55745; B55745.
DR RefSeq; NP_477039.1; NM_057691.4. [Q24318-1]
DR RefSeq; NP_725267.1; NM_165974.3. [Q24318-2]
DR AlphaFoldDB; Q24318; -.
DR SMR; Q24318; -.
DR BioGRID; 62224; 31.
DR IntAct; Q24318; 11.
DR STRING; 7227.FBpp0086853; -.
DR iPTMnet; Q24318; -.
DR PaxDb; Q24318; -.
DR PRIDE; Q24318; -.
DR DNASU; 36461; -.
DR EnsemblMetazoa; FBtr0087740; FBpp0086853; FBgn0011763. [Q24318-1]
DR EnsemblMetazoa; FBtr0087741; FBpp0086854; FBgn0011763. [Q24318-2]
DR GeneID; 36461; -.
DR KEGG; dme:Dmel_CG4654; -.
DR CTD; 13450; -.
DR FlyBase; FBgn0011763; Dp.
DR VEuPathDB; VectorBase:FBgn0011763; -.
DR eggNOG; KOG2829; Eukaryota.
DR GeneTree; ENSGT00940000169233; -.
DR InParanoid; Q24318; -.
DR OMA; NNPMKIK; -.
DR PhylomeDB; Q24318; -.
DR Reactome; R-DME-1538133; G0 and Early G1.
DR Reactome; R-DME-2173796; SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
DR Reactome; R-DME-69231; Cyclin D associated events in G1.
DR Reactome; R-DME-8953750; Transcriptional Regulation by E2F6.
DR BioGRID-ORCS; 36461; 1 hit in 3 CRISPR screens.
DR ChiTaRS; Dp; fly.
DR GenomeRNAi; 36461; -.
DR PRO; PR:Q24318; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0011763; Expressed in oocyte and 53 other tissues.
DR ExpressionAtlas; Q24318; baseline and differential.
DR Genevisible; Q24318; DM.
DR GO; GO:0031523; C:Myb complex; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; HDA:FlyBase.
DR GO; GO:0035189; C:Rb-E2F complex; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; IDA:FlyBase.
DR GO; GO:0008134; F:transcription factor binding; IPI:FlyBase.
DR GO; GO:0008069; P:dorsal/ventral axis specification, ovarian follicular epithelium; IMP:FlyBase.
DR GO; GO:0007307; P:eggshell chorion gene amplification; TAS:FlyBase.
DR GO; GO:0007113; P:endomitotic cell cycle; TAS:FlyBase.
DR GO; GO:0008630; P:intrinsic apoptotic signaling pathway in response to DNA damage; IMP:FlyBase.
DR GO; GO:0045476; P:nurse cell apoptotic process; TAS:FlyBase.
DR GO; GO:0048477; P:oogenesis; IMP:FlyBase.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:FlyBase.
DR GO; GO:0045850; P:positive regulation of nurse cell apoptotic process; IMP:FlyBase.
DR GO; GO:0051726; P:regulation of cell cycle; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd14458; DP_DD; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.20.140.80; -; 1.
DR InterPro; IPR037241; E2F-DP_heterodim.
DR InterPro; IPR003316; E2F_WHTH_DNA-bd_dom.
DR InterPro; IPR038168; TF_DP_C_sf.
DR InterPro; IPR014889; Transc_factor_DP_C.
DR InterPro; IPR015648; Transcrpt_fac_DP.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR12548; PTHR12548; 1.
DR Pfam; PF08781; DP; 1.
DR Pfam; PF02319; E2F_TDP; 1.
DR PIRSF; PIRSF009404; Transcription_factor_DP; 1.
DR SMART; SM01138; DP; 1.
DR SMART; SM01372; E2F_TDP; 1.
DR SUPFAM; SSF144074; SSF144074; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..445
FT /note="Transcription factor Dp"
FT /id="PRO_0000219479"
FT REGION 145..168
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 392..445
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 408..426
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 398
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT VAR_SEQ 1..22
FT /note="MAHSTGGTVKTDEVNFFFRNEH -> MCISETKEPPHLPISNSA (in
FT isoform B)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_014146"
FT CONFLICT 117
FT /note="D -> G (in Ref. 4; AAO24938)"
FT /evidence="ECO:0000305"
FT CONFLICT 343
FT /note="H -> L (in Ref. 6; AAB87765)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 445 AA; 49750 MW; 87F4DBB26CFE4E42 CRC64;
MAHSTGGTVK TDEVNFFFRN EHGQISKMLK PAQNKSEMEG GKPVAVVYAT GSSARNSGSA
SGIGNVGRMG AFSQMGSQGQ FIRLQDNGLS IPKTEAGTTY TTVSAQKTSG AGSGHYDLPL
KGDRYVKFTP NPIKMKSKLH AIQSNSLHSM SASSSSVQRK RKPDKAGKGL RHFSMKVCEK
VEEKGKTTYN EVADDLVSEE MKNNAYDNNC DQKNIRRRVY DALNVLMAIN VISKDKKEIR
WIGLPANSTE TFLALEEENC QRRERIKQKN EMLREMIMQH VAFKGLVERN KRNESQGVVP
SPNASIQLPF IIVNTHKSTK INCSVTNDKS EYIFKFDKTF EMHDDIEVLK RMGFLLGLDK
GECTPENIER VKSWVPPNLA KYVEAYGTGK TGENMYESDD EDNEFNGYLE SANESQGFAQ
HSAQHTTDGE FKLEMDDDEL DDDID
