TFE_METHJ
ID TFE_METHJ Reviewed; 173 AA.
AC Q2FT22;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 25-MAY-2022, entry version 87.
DE RecName: Full=Transcription factor E {ECO:0000255|HAMAP-Rule:MF_01909};
DE Short=TFE {ECO:0000255|HAMAP-Rule:MF_01909};
DE AltName: Full=TFIIE subunit alpha homolog {ECO:0000255|HAMAP-Rule:MF_01909};
DE AltName: Full=Transcription initiation factor TFIIE {ECO:0000255|HAMAP-Rule:MF_01909};
GN Name=tfe {ECO:0000255|HAMAP-Rule:MF_01909}; OrderedLocusNames=Mhun_2797;
OS Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 /
OS JF-1).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanomicrobiales; Methanospirillaceae; Methanospirillum.
OX NCBI_TaxID=323259;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27890 / DSM 864 / NBRC 100397 / JF-1;
RX PubMed=26744606; DOI=10.1186/s40793-015-0124-8;
RA Gunsalus R.P., Cook L.E., Crable B., Rohlin L., McDonald E., Mouttaki H.,
RA Sieber J.R., Poweleit N., Zhou H., Lapidus A.L., Daligault H.E., Land M.,
RA Gilna P., Ivanova N., Kyrpides N., Culley D.E., McInerney M.J.;
RT "Complete genome sequence of Methanospirillum hungatei type strain JF1.";
RL Stand. Genomic Sci. 11:2-2(2016).
CC -!- FUNCTION: Transcription factor that plays a role in the activation of
CC archaeal genes transcribed by RNA polymerase. Facilitates transcription
CC initiation by enhancing TATA-box recognition by TATA-box-binding
CC protein (Tbp), and transcription factor B (Tfb) and RNA polymerase
CC recruitment. Not absolutely required for transcription in vitro, but
CC particularly important in cases where Tbp or Tfb function is not
CC optimal. It dynamically alters the nucleic acid-binding properties of
CC RNA polymerases by stabilizing the initiation complex and destabilizing
CC elongation complexes. Seems to translocate with the RNA polymerase
CC following initiation and acts by binding to the non template strand of
CC the transcription bubble in elongation complexes. {ECO:0000255|HAMAP-
CC Rule:MF_01909}.
CC -!- SUBUNIT: Monomer. Interaction with RNA polymerase subunits RpoF and
CC RpoE is necessary for Tfe stimulatory transcription activity. Able to
CC interact with Tbp and RNA polymerase in the absence of DNA promoter.
CC Interacts both with the preinitiation and elongation complexes.
CC {ECO:0000255|HAMAP-Rule:MF_01909}.
CC -!- DOMAIN: The winged helix domain is involved in binding to DNA in the
CC preinitiation complex. {ECO:0000255|HAMAP-Rule:MF_01909}.
CC -!- SIMILARITY: Belongs to the TFE family. {ECO:0000255|HAMAP-
CC Rule:MF_01909}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000254; ABD42491.1; -; Genomic_DNA.
DR RefSeq; WP_011449746.1; NC_007796.1.
DR AlphaFoldDB; Q2FT22; -.
DR SMR; Q2FT22; -.
DR STRING; 323259.Mhun_2797; -.
DR EnsemblBacteria; ABD42491; ABD42491; Mhun_2797.
DR GeneID; 3923035; -.
DR KEGG; mhu:Mhun_2797; -.
DR eggNOG; arCOG04270; Archaea.
DR HOGENOM; CLU_100097_0_0_2; -.
DR OMA; DSGWLTY; -.
DR OrthoDB; 75288at2157; -.
DR Proteomes; UP000001941; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IEA:InterPro.
DR Gene3D; 1.10.10.10; -; 1.
DR HAMAP; MF_01909; TFE_arch; 1.
DR InterPro; IPR016481; TF_E_archaea.
DR InterPro; IPR039997; TFE.
DR InterPro; IPR017919; TFIIE/TFIIEa_HTH.
DR InterPro; IPR002853; TFIIE_asu.
DR InterPro; IPR024550; TFIIEa/SarR/Rpc3_HTH_dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR13097; PTHR13097; 1.
DR Pfam; PF02002; TFIIE_alpha; 1.
DR PIRSF; PIRSF006373; TF_E_archaea; 1.
DR SMART; SM00531; TFIIE; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS51344; HTH_TFE_IIE; 1.
PE 3: Inferred from homology;
KW DNA-binding; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..173
FT /note="Transcription factor E"
FT /id="PRO_0000326612"
FT DOMAIN 9..92
FT /note="HTH TFE/IIEalpha-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01909"
SQ SEQUENCE 173 AA; 20515 MW; 999AB70F1146C22D CRC64;
MASHEEILAD KAIFAYLHRM IGDEGIELIR RFPTDKEYSD EELAEVTKIN LNSVRNTLYT
LYEHRLAKYR RIKNNETGWL TYLWELELDN MYDSVSKDLE IILEKLRKRY KYESENAFYN
CPNCGNTITF SDAMDSQFVC QECENKMVHF DNDLLVNALQ KRIARIEENL GHV
