TFE_METST
ID TFE_METST Reviewed; 179 AA.
AC Q2NEV4;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 07-FEB-2006, sequence version 1.
DT 25-MAY-2022, entry version 96.
DE RecName: Full=Transcription factor E {ECO:0000255|HAMAP-Rule:MF_01909};
DE Short=TFE {ECO:0000255|HAMAP-Rule:MF_01909};
DE AltName: Full=TFIIE subunit alpha homolog {ECO:0000255|HAMAP-Rule:MF_01909};
DE AltName: Full=Transcription initiation factor TFIIE {ECO:0000255|HAMAP-Rule:MF_01909};
GN Name=tfe {ECO:0000255|HAMAP-Rule:MF_01909}; OrderedLocusNames=Msp_1272;
OS Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 /
OS MCB-3).
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanosphaera.
OX NCBI_TaxID=339860;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3;
RX PubMed=16385054; DOI=10.1128/jb.188.2.642-658.2006;
RA Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R.,
RA Gottschalk G., Thauer R.K.;
RT "The genome sequence of Methanosphaera stadtmanae reveals why this human
RT intestinal archaeon is restricted to methanol and H2 for methane formation
RT and ATP synthesis.";
RL J. Bacteriol. 188:642-658(2006).
CC -!- FUNCTION: Transcription factor that plays a role in the activation of
CC archaeal genes transcribed by RNA polymerase. Facilitates transcription
CC initiation by enhancing TATA-box recognition by TATA-box-binding
CC protein (Tbp), and transcription factor B (Tfb) and RNA polymerase
CC recruitment. Not absolutely required for transcription in vitro, but
CC particularly important in cases where Tbp or Tfb function is not
CC optimal. It dynamically alters the nucleic acid-binding properties of
CC RNA polymerases by stabilizing the initiation complex and destabilizing
CC elongation complexes. Seems to translocate with the RNA polymerase
CC following initiation and acts by binding to the non template strand of
CC the transcription bubble in elongation complexes. {ECO:0000255|HAMAP-
CC Rule:MF_01909}.
CC -!- SUBUNIT: Monomer. Interaction with RNA polymerase subunits RpoF and
CC RpoE is necessary for Tfe stimulatory transcription activity. Able to
CC interact with Tbp and RNA polymerase in the absence of DNA promoter.
CC Interacts both with the preinitiation and elongation complexes.
CC {ECO:0000255|HAMAP-Rule:MF_01909}.
CC -!- DOMAIN: The winged helix domain is involved in binding to DNA in the
CC preinitiation complex. {ECO:0000255|HAMAP-Rule:MF_01909}.
CC -!- SIMILARITY: Belongs to the TFE family. {ECO:0000255|HAMAP-
CC Rule:MF_01909}.
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DR EMBL; CP000102; ABC57649.1; -; Genomic_DNA.
DR RefSeq; WP_011406848.1; NC_007681.1.
DR AlphaFoldDB; Q2NEV4; -.
DR SMR; Q2NEV4; -.
DR STRING; 339860.Msp_1272; -.
DR EnsemblBacteria; ABC57649; ABC57649; Msp_1272.
DR GeneID; 41325842; -.
DR KEGG; mst:Msp_1272; -.
DR eggNOG; arCOG04270; Archaea.
DR HOGENOM; CLU_100097_0_0_2; -.
DR OMA; FENDFLC; -.
DR OrthoDB; 75288at2157; -.
DR Proteomes; UP000001931; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IEA:InterPro.
DR Gene3D; 1.10.10.10; -; 1.
DR HAMAP; MF_01909; TFE_arch; 1.
DR InterPro; IPR016481; TF_E_archaea.
DR InterPro; IPR017919; TFIIE/TFIIEa_HTH.
DR InterPro; IPR002853; TFIIE_asu.
DR InterPro; IPR024550; TFIIEa/SarR/Rpc3_HTH_dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF02002; TFIIE_alpha; 1.
DR PIRSF; PIRSF006373; TF_E_archaea; 1.
DR SMART; SM00531; TFIIE; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR TIGRFAMs; TIGR00373; TIGR00373; 1.
DR PROSITE; PS51344; HTH_TFE_IIE; 1.
PE 3: Inferred from homology;
KW DNA-binding; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..179
FT /note="Transcription factor E"
FT /id="PRO_0000326611"
FT DOMAIN 1..102
FT /note="HTH TFE/IIEalpha-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01909"
SQ SEQUENCE 179 AA; 21533 MW; 350FDBE9B9513423 CRC64;
MAKKKVKYTF DYDSKFLNEH NVKKLAYELT HDSDTSNKIL DCLFTAEVTD EQIAEATGIK
LNFVRKILYK FYDVGMANYT RKKDPETQWF TYYWRFDSRK AAQILEKQYN HHNQEIKESI
EYEENNMFFV CPNGCRYPFD EATEFQFICP RCNEKLEFKD NSDLIHDLKK LESAYKINE