ID   TFF2_RAT                Reviewed;         129 AA.
AC   Q09030;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   25-MAY-2022, entry version 112.
DE   RecName: Full=Trefoil factor 2;
DE   AltName: Full=Spasmolytic polypeptide;
DE            Short=SP;
DE   Flags: Precursor;
GN   Name=Tff2; Synonyms=Sml1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Gastric antrum;
RX   PubMed=8299900; DOI=10.1016/0016-5085(94)90590-8;
RA   Jeffrey G.P., Oates P.S., Wang T.C., Babyatsky M.W., Brand S.J.;
RT   "Spasmolytic polypeptide: a trefoil peptide secreted by rat gastric mucous
RT   cells.";
RL   Gastroenterology 106:336-345(1994).
CC   -!- FUNCTION: Inhibits gastrointestinal motility and gastric acid
CC       secretion. Could function as a structural component of gastric mucus,
CC       possibly by stabilizing glycoproteins in the mucus gel through
CC       interactions with carbohydrate side chains.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed in the digestive tract, where it was
CC       found predominantly in the stomach with highest expression in the
CC       antrum. It is secreted predominantly from antral mucous cells into the
CC       lumen of the gastrointestinal tract.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M97255; AAA19025.1; -; mRNA.
DR   PIR; I53637; I53637.
DR   RefSeq; NP_446296.1; NM_053844.1.
DR   AlphaFoldDB; Q09030; -.
DR   SMR; Q09030; -.
DR   STRING; 10116.ENSRNOP00000001537; -.
DR   PaxDb; Q09030; -.
DR   GeneID; 116592; -.
DR   KEGG; rno:116592; -.
DR   UCSC; RGD:620709; rat.
DR   CTD; 7032; -.
DR   RGD; 620709; Tff2.
DR   eggNOG; ENOG502S5ZY; Eukaryota.
DR   InParanoid; Q09030; -.
DR   OrthoDB; 1563185at2759; -.
DR   PhylomeDB; Q09030; -.
DR   PRO; PR:Q09030; -.
DR   Proteomes; UP000002494; Unplaced.
DR   GO; GO:0005615; C:extracellular space; ISO:RGD.
DR   GO; GO:0031723; F:CXCR4 chemokine receptor binding; ISO:RGD.
DR   GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0005198; F:structural molecule activity; NAS:RGD.
DR   GO; GO:0019722; P:calcium-mediated signaling; ISO:RGD.
DR   GO; GO:0070098; P:chemokine-mediated signaling pathway; ISO:RGD.
DR   GO; GO:0030277; P:maintenance of gastrointestinal epithelium; IBA:GO_Central.
DR   GO; GO:0060455; P:negative regulation of gastric acid secretion; ISO:RGD.
DR   GO; GO:0050728; P:negative regulation of inflammatory response; ISO:RGD.
DR   GO; GO:0043031; P:negative regulation of macrophage activation; ISO:RGD.
DR   GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:RGD.
DR   GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; ISO:RGD.
DR   GO; GO:0030334; P:regulation of cell migration; ISO:RGD.
DR   CDD; cd00111; Trefoil; 2.
DR   Gene3D; 4.10.110.10; -; 2.
DR   InterPro; IPR017994; P_trefoil_chordata.
DR   InterPro; IPR017957; P_trefoil_CS.
DR   InterPro; IPR000519; P_trefoil_dom.
DR   InterPro; IPR044913; P_trefoil_dom_sf.
DR   PANTHER; PTHR13826; PTHR13826; 1.
DR   Pfam; PF00088; Trefoil; 2.
DR   PRINTS; PR00680; PTREFOIL.
DR   SMART; SM00018; PD; 2.
DR   SUPFAM; SSF57492; SSF57492; 2.
DR   PROSITE; PS00025; P_TREFOIL_1; 2.
DR   PROSITE; PS51448; P_TREFOIL_2; 2.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Growth factor; Reference proteome; Repeat; Secreted;
KW   Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..129
FT                   /note="Trefoil factor 2"
FT                   /id="PRO_0000023463"
FT   DOMAIN          29..73
FT                   /note="P-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00779"
FT   DOMAIN          79..122
FT                   /note="P-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00779"
FT   DISULFID        29..127
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00779"
FT   DISULFID        31..58
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00779"
FT   DISULFID        42..57
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00779"
FT   DISULFID        52..69
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00779"
FT   DISULFID        81..107
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00779"
FT   DISULFID        91..106
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00779"
FT   DISULFID        101..118
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00779"
SQ   SEQUENCE   129 AA;  14077 MW;  005D0848F64F92D2 CRC64;
     MGPRGAPLLV VVLVLGLHAL AEGEKPSPCR CSRMTPSNRK NCGFPGITSD QCFNLGCCFD
     SSVAGVPWCF HPLPNQASEQ CVMEVSAREN CGYPGISPED CASRHCCFSN LIFEVPWCFF
     PQSVDDCHY