TFF3_MOUSE
ID TFF3_MOUSE Reviewed; 81 AA.
AC Q62395; Q64352;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 2.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Trefoil factor 3;
DE AltName: Full=Intestinal trefoil factor;
DE Short=mITF;
DE Flags: Precursor;
GN Name=Tff3; Synonyms=Itf;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, AND TISSUE
RP SPECIFICITY.
RC STRAIN=129/Sv; TISSUE=Liver;
RX PubMed=7741746; DOI=10.1006/bbrc.1995.1623;
RA Mashimo H., Podolsky D.K., Fishman M.C.;
RT "Structure and expression of murine intestinal trefoil factor: high
RT evolutionary conservation and postnatal expression.";
RL Biochem. Biophys. Res. Commun. 210:31-37(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J; TISSUE=Small intestine;
RX PubMed=7575467; DOI=10.1042/bj3110293;
RA Tomita M., Itoh H., Ishikawa N., Higa A., Ide H., Murakumo Y., Maruyama H.,
RA Koga Y., Nawa Y.;
RT "Molecular cloning of mouse intestinal trefoil factor and its expression
RT during goblet cell changes.";
RL Biochem. J. 311:293-297(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11290420; DOI=10.1016/s0378-1119(01)00380-8;
RA Ribieras S., Lefebvre O., Tomasetto C., Rio M.C.;
RT "Mouse trefoil factor genes: genomic organization, sequences and
RT methylation analyses.";
RL Gene 266:67-75(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-77.
RX PubMed=8666281; DOI=10.1016/0378-1119(96)00074-1;
RA Chinery R., Poulsom R., Cox H.M.;
RT "The gene encoding mouse intestinal trefoil factor: structural
RT organization, partial sequence analysis and mapping to murine chromosome
RT 17q.";
RL Gene 171:249-253(1996).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=8824194; DOI=10.1126/science.274.5285.262;
RA Mashimo H., Wu D.C., Podolsky D.K., Fishman M.C.;
RT "Impaired defense of intestinal mucosa in mice lacking intestinal trefoil
RT factor.";
RL Science 274:262-265(1996).
CC -!- FUNCTION: Involved in the maintenance and repair of the intestinal
CC mucosa. Promotes the mobility of epithelial cells in healing processes
CC (motogen). {ECO:0000269|PubMed:8824194}.
CC -!- SUBUNIT: Monomer. Homodimer; disulfide-linked. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix {ECO:0000250|UniProtKB:Q07654}. Cytoplasm
CC {ECO:0000250|UniProtKB:Q07654}.
CC -!- TISSUE SPECIFICITY: Expressed in goblet cells of the intestines and
CC colon (at protein level). Expressed abundantly in goblet cells of
CC intestine and colon, and at low levels in stomach. No expression in
CC brain, lung, spleen, kidney, uterus, pancreas, liver, heart or thymus.
CC {ECO:0000269|PubMed:7575467, ECO:0000269|PubMed:7741746}.
CC -!- DEVELOPMENTAL STAGE: No expression found in embryos.
CC {ECO:0000269|PubMed:7741746}.
CC -!- DISRUPTION PHENOTYPE: Mice lacking this gene show impaired mucosal
CC healing after injury and die from extensive colitis after oral
CC administration of dextran sulfate. {ECO:0000269|PubMed:8824194}.
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DR EMBL; U24222; AAB92385.1; -; Genomic_DNA.
DR EMBL; U24220; AAB92385.1; JOINED; Genomic_DNA.
DR EMBL; U24221; AAB92385.1; JOINED; Genomic_DNA.
DR EMBL; D38410; BAA07464.1; -; mRNA.
DR EMBL; AJ271004; CAB65752.1; -; Genomic_DNA.
DR EMBL; BC011042; AAH11042.1; -; mRNA.
DR EMBL; U46858; AAC52682.1; -; Genomic_DNA.
DR CCDS; CCDS37544.1; -.
DR PIR; JC4147; JC4147.
DR RefSeq; NP_035705.1; NM_011575.2.
DR AlphaFoldDB; Q62395; -.
DR SMR; Q62395; -.
DR STRING; 10090.ENSMUSP00000024827; -.
DR MaxQB; Q62395; -.
DR PaxDb; Q62395; -.
DR PeptideAtlas; Q62395; -.
DR PRIDE; Q62395; -.
DR ProteomicsDB; 259018; -.
DR DNASU; 21786; -.
DR Ensembl; ENSMUST00000024827; ENSMUSP00000024827; ENSMUSG00000024029.
DR GeneID; 21786; -.
DR KEGG; mmu:21786; -.
DR UCSC; uc008buk.2; mouse.
DR CTD; 7033; -.
DR MGI; MGI:104638; Tff3.
DR VEuPathDB; HostDB:ENSMUSG00000024029; -.
DR eggNOG; ENOG502SV7V; Eukaryota.
DR GeneTree; ENSGT00940000162416; -.
DR HOGENOM; CLU_179440_0_0_1; -.
DR InParanoid; Q62395; -.
DR OMA; QETECTF; -.
DR OrthoDB; 1563185at2759; -.
DR PhylomeDB; Q62395; -.
DR TreeFam; TF336092; -.
DR BioGRID-ORCS; 21786; 1 hit in 71 CRISPR screens.
DR PRO; PR:Q62395; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q62395; protein.
DR Bgee; ENSMUSG00000024029; Expressed in crypt of Lieberkuhn of small intestine and 67 other tissues.
DR Genevisible; Q62395; MM.
DR GO; GO:0005576; C:extracellular region; ISO:MGI.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0030141; C:secretory granule; IDA:MGI.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0030277; P:maintenance of gastrointestinal epithelium; IBA:GO_Central.
DR GO; GO:0010906; P:regulation of glucose metabolic process; IDA:CACAO.
DR CDD; cd00111; Trefoil; 1.
DR Gene3D; 4.10.110.10; -; 1.
DR InterPro; IPR017994; P_trefoil_chordata.
DR InterPro; IPR017957; P_trefoil_CS.
DR InterPro; IPR000519; P_trefoil_dom.
DR InterPro; IPR044913; P_trefoil_dom_sf.
DR PANTHER; PTHR13826; PTHR13826; 1.
DR Pfam; PF00088; Trefoil; 1.
DR PRINTS; PR00680; PTREFOIL.
DR SMART; SM00018; PD; 1.
DR SUPFAM; SSF57492; SSF57492; 1.
DR PROSITE; PS00025; P_TREFOIL_1; 1.
DR PROSITE; PS51448; P_TREFOIL_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Disulfide bond; Extracellular matrix; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..81
FT /note="Trefoil factor 3"
FT /id="PRO_0000023466"
FT DOMAIN 31..74
FT /note="P-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00779"
FT DISULFID 33..59
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00779"
FT DISULFID 43..58
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00779"
FT DISULFID 53..70
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00779"
FT DISULFID 79
FT /note="Interchain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00779"
SQ SEQUENCE 81 AA; 8808 MW; 655A2E00E390C036 CRC64;
METRALWLML LVVLVAGSSG IAADYVGLSP SQCMVPANVR VDCGYPSVTS EQCNNRGCCF
DSSIPNVPWC FKPLQETECT F
