TFIIS_ASFB7
ID TFIIS_ASFB7 Reviewed; 243 AA.
AC P27948;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Transcription factor TFIIS homolog {ECO:0000303|PubMed:11831707};
GN OrderedLocusNames=BA71V-140; ORFNames=I243L;
OS African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V)
OS (ASFV).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Asfuvirales; Asfarviridae; Asfivirus.
OX NCBI_TaxID=10498;
OH NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH NCBI_TaxID=9823; Sus scrofa (Pig).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=1309282; DOI=10.1016/0042-6822(92)90059-x;
RA Rodriguez J.M., Salas M.L., Vinuela E.;
RT "Genes homologous to ubiquitin-conjugating proteins and eukaryotic
RT transcription factor SII in African swine fever virus.";
RL Virology 186:40-52(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11831707; DOI=10.1006/viro.1995.1149;
RA Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C.,
RA Rodriguez J.F., Vinuela E.;
RT "Analysis of the complete nucleotide sequence of African swine fever
RT virus.";
RL Virology 208:249-278(1995).
RN [3]
RP REVIEW.
RX PubMed=23041356; DOI=10.1016/j.virusres.2012.09.014;
RA Rodriguez J.M., Salas M.L.;
RT "African swine fever virus transcription.";
RL Virus Res. 173:15-28(2013).
RN [4]
RP REVIEW.
RX PubMed=32725217; DOI=10.1042/bst20191108;
RA Cackett G., Sykora M., Werner F.;
RT "Transcriptome view of a killer: African swine fever virus.";
RL Biochem. Soc. Trans. 48:1569-1581(2020).
RN [5]
RP ALTERNATIVE INITIATION.
RX PubMed=32075923; DOI=10.1128/jvi.00119-20;
RA Cackett G., Matelska D., Sykora M., Portugal R., Malecki M., Baehler J.,
RA Dixon L., Werner F.;
RT "The African Swine Fever Virus Transcriptome.";
RL J. Virol. 94:0-0(2020).
CC -!- FUNCTION: Putative initiation factor. Necessary for efficient
CC transcription elongation past template-encoded arresting sites.
CC {ECO:0000250|UniProtKB:P07273}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=3;
CC Comment=Isoform 1 contains the N-terminal region that is probably
CC responsible for the initiation function (PubMed:32075923).
CC {ECO:0000269|PubMed:32075923};
CC Name=1;
CC IsoId=P27948-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P27948-2; Sequence=VSP_061337;
CC Name=3;
CC IsoId=P27948-3; Sequence=VSP_061336;
CC -!- INDUCTION: [Isoform 1]: Expressed in the intermediate phase of the
CC viral replicative cycle. {ECO:0000269|PubMed:32075923}.
CC -!- INDUCTION: [Isoform 2]: Expressed in the early phase of the viral
CC replicative cycle. {ECO:0000269|PubMed:32075923}.
CC -!- INDUCTION: [Isoform 3]: Expressed in the late phase of the viral
CC replicative cycle. {ECO:0000269|PubMed:32075923}.
CC -!- SIMILARITY: Belongs to the TFS-II family. {ECO:0000305}.
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DR EMBL; M77121; AAA42700.1; -; Genomic_DNA.
DR EMBL; U18466; AAA65367.1; -; Genomic_DNA.
DR PIR; B39448; WMXFB2.
DR RefSeq; NP_042831.1; NC_001659.2.
DR GeneID; 22220367; -.
DR KEGG; vg:22220367; -.
DR Proteomes; UP000000624; Genome.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR InterPro; IPR035100; TF_IIS-typ.
DR InterPro; IPR003618; TFIIS_cen_dom.
DR InterPro; IPR001222; Znf_TFIIS.
DR Pfam; PF01096; TFIIS_C; 1.
DR PIRSF; PIRSF006704; TF_IIS; 1.
DR SMART; SM00510; TFS2M; 1.
DR SMART; SM00440; ZnF_C2C2; 1.
DR PROSITE; PS51321; TFIIS_CENTRAL; 1.
DR PROSITE; PS00466; ZF_TFIIS_1; 1.
DR PROSITE; PS51133; ZF_TFIIS_2; 1.
PE 2: Evidence at transcript level;
KW Alternative initiation; Early protein; Late protein; Metal-binding;
KW Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..243
FT /note="Transcription factor TFIIS homolog"
FT /id="PRO_0000121454"
FT DOMAIN 77..201
FT /note="TFIIS central"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00651"
FT ZN_FING 202..242
FT /note="TFIIS-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00472"
FT BINDING 206
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00472"
FT BINDING 209
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00472"
FT BINDING 234
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00472"
FT BINDING 237
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00472"
FT VAR_SEQ 1..52
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000269|PubMed:32075923"
FT /id="VSP_061336"
FT VAR_SEQ 1..2
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000269|PubMed:32075923"
FT /id="VSP_061337"
SQ SEQUENCE 243 AA; 28558 MW; 48D8FFD2E9C8128F CRC64;
MKMHIARDSI VFLLNKHLQN TILTNKIEQE CFLQADTPKK YLQYIKPFLI NCMTKNITTD
LVMKDSKRLE PYIILEMRDI IQMMFFRTLQ KHMFFKEHTD LCTEYAQKIE ASCYHYTYQQ
QEKTFLEEYS TRCGTINHII NCEKKSHQQQ DNDALNKLIS GELKPEAIGS MTFAELCPSA
ALKEKTEITL RSQQKVAEKT SQLYKCPNCK QRMCTYREVQ TRALDEPSTI FCTCKKCGHE
FIG
