TFIP8_MOUSE
ID TFIP8_MOUSE Reviewed; 198 AA.
AC Q921Z5; Q3TQS5; Q3U9V7; Q3UBG8; Q8BTH4;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Tumor necrosis factor alpha-induced protein 8;
DE Short=TNF alpha-induced protein 8;
GN Name=Tnfaip8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Bone marrow, Egg, and Lung;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, Kidney, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Acts as a negative mediator of apoptosis. Suppresses the TNF-
CC mediated apoptosis by inhibiting caspase-8 activity but not the
CC processing of procaspase-8, subsequently resulting in inhibition of BID
CC cleavage and caspase-3 activation (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q921Z5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q921Z5-2; Sequence=VSP_024900, VSP_024901;
CC -!- SIMILARITY: Belongs to the TNFAIP8 family. {ECO:0000305}.
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DR EMBL; AK090316; BAC41167.1; -; mRNA.
DR EMBL; AK149901; BAE29154.1; -; mRNA.
DR EMBL; AK150445; BAE29567.1; -; mRNA.
DR EMBL; AK150965; BAE29996.1; -; mRNA.
DR EMBL; AK151622; BAE30559.1; -; mRNA.
DR EMBL; AK152426; BAE31209.1; -; mRNA.
DR EMBL; AK163339; BAE37307.1; -; mRNA.
DR EMBL; BC009090; AAH09090.1; -; mRNA.
DR CCDS; CCDS50287.1; -. [Q921Z5-2]
DR RefSeq; NP_001171231.1; NM_001177760.1. [Q921Z5-2]
DR RefSeq; NP_598892.2; NM_134131.2.
DR PDB; 5JXD; X-ray; 2.03 A; A=1-198.
DR PDBsum; 5JXD; -.
DR AlphaFoldDB; Q921Z5; -.
DR SMR; Q921Z5; -.
DR BioGRID; 223149; 22.
DR IntAct; Q921Z5; 1.
DR MINT; Q921Z5; -.
DR STRING; 10090.ENSMUSP00000119041; -.
DR PhosphoSitePlus; Q921Z5; -.
DR EPD; Q921Z5; -.
DR jPOST; Q921Z5; -.
DR MaxQB; Q921Z5; -.
DR PaxDb; Q921Z5; -.
DR PeptideAtlas; Q921Z5; -.
DR PRIDE; Q921Z5; -.
DR ProteomicsDB; 262800; -. [Q921Z5-1]
DR ProteomicsDB; 262801; -. [Q921Z5-2]
DR Antibodypedia; 25539; 268 antibodies from 32 providers.
DR DNASU; 106869; -.
DR Ensembl; ENSMUST00000179937; ENSMUSP00000136030; ENSMUSG00000062210. [Q921Z5-2]
DR GeneID; 106869; -.
DR KEGG; mmu:106869; -.
DR UCSC; uc008ewq.2; mouse. [Q921Z5-2]
DR CTD; 25816; -.
DR MGI; MGI:2147191; Tnfaip8.
DR VEuPathDB; HostDB:ENSMUSG00000062210; -.
DR eggNOG; ENOG502S00N; Eukaryota.
DR GeneTree; ENSGT00390000003488; -.
DR HOGENOM; CLU_1942921_0_0_1; -.
DR InParanoid; Q921Z5; -.
DR OrthoDB; 1284744at2759; -.
DR Reactome; R-MMU-1483255; PI Metabolism.
DR BioGRID-ORCS; 106869; 3 hits in 73 CRISPR screens.
DR ChiTaRS; Tnfaip8; mouse.
DR PRO; PR:Q921Z5; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; Q921Z5; protein.
DR Bgee; ENSMUSG00000062210; Expressed in gastrula and 259 other tissues.
DR ExpressionAtlas; Q921Z5; baseline and differential.
DR Genevisible; Q921Z5; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0043027; F:cysteine-type endopeptidase inhibitor activity involved in apoptotic process; ISS:UniProtKB.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IMP:MGI.
DR GO; GO:0043065; P:positive regulation of apoptotic process; ISS:UniProtKB.
DR DisProt; DP02802; -.
DR Gene3D; 1.20.1440.160; -; 1.
DR InterPro; IPR008477; TNFAIP8-like.
DR InterPro; IPR038355; TNFAIP8_sf.
DR PANTHER; PTHR12757; PTHR12757; 1.
DR Pfam; PF05527; DUF758; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Apoptosis; Coiled coil; Cytoplasm;
KW Reference proteome.
FT CHAIN 1..198
FT /note="Tumor necrosis factor alpha-induced protein 8"
FT /id="PRO_0000285719"
FT COILED 49..83
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..11
FT /note="MLSEAEEPREV -> M (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024900"
FT VAR_SEQ 119..198
FT /note="VEYTFDRNVLSRLLNECRELLHEIIQRHLTAKSHGRVNNVFDHFSDCDFLAA
FT LYNPFGKFKPHLQKLCDGINKMLDEENI -> IVIDLQELNLRQSNRMLRKAKKTVHLK
FT S (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024901"
FT CONFLICT 19
FT /note="K -> Q (in Ref. 1; BAE29996)"
FT /evidence="ECO:0000305"
FT CONFLICT 104
FT /note="K -> N (in Ref. 1; BAE30559/BAE31209/BAE29567)"
FT /evidence="ECO:0000305"
FT CONFLICT 190
FT /note="N -> K (in Ref. 1; BAC41167)"
FT /evidence="ECO:0000305"
FT HELIX 19..33
FT /evidence="ECO:0007829|PDB:5JXD"
FT HELIX 35..39
FT /evidence="ECO:0007829|PDB:5JXD"
FT TURN 40..42
FT /evidence="ECO:0007829|PDB:5JXD"
FT HELIX 45..62
FT /evidence="ECO:0007829|PDB:5JXD"
FT HELIX 65..87
FT /evidence="ECO:0007829|PDB:5JXD"
FT HELIX 93..118
FT /evidence="ECO:0007829|PDB:5JXD"
FT HELIX 125..143
FT /evidence="ECO:0007829|PDB:5JXD"
FT TURN 144..146
FT /evidence="ECO:0007829|PDB:5JXD"
FT HELIX 149..162
FT /evidence="ECO:0007829|PDB:5JXD"
FT HELIX 165..171
FT /evidence="ECO:0007829|PDB:5JXD"
FT HELIX 179..194
FT /evidence="ECO:0007829|PDB:5JXD"
SQ SEQUENCE 198 AA; 22960 MW; B6E8DCC5CDC25F6A CRC64;
MLSEAEEPRE VATDVFNSKN LAVQAQKKIL GKMVSKSIAT TLIDDTSSEV LDELYRVTKE
YTQNKKEAER VIKNLIKTVI KLAVLHRNNQ FNQDELALME KFKKKVHQLA MTVVSFHQVE
YTFDRNVLSR LLNECRELLH EIIQRHLTAK SHGRVNNVFD HFSDCDFLAA LYNPFGKFKP
HLQKLCDGIN KMLDEENI
