TFL1_ARATH
ID TFL1_ARATH Reviewed; 177 AA.
AC P93003; O04756; O04757; O04758;
DT 05-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Protein TERMINAL FLOWER 1;
GN Name=TFL1; OrderedLocusNames=At5g03840; ORFNames=F8F6_50;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND MUTANTS TLF1-1; TFL1-11; TFL1-13 AND
RP TFL1-14.
RC STRAIN=cv. Columbia;
RX PubMed=8974397; DOI=10.1126/science.275.5296.80;
RA Bradley D., Ratcliffe O., Vincent C., Carpenter R., Coen E.S.;
RT "Inflorescence commitment and architecture in Arabidopsis.";
RL Science 275:80-83(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTANTS TLF1-1; TFL1-11; TFL1-13 AND
RP TFL1-14.
RC STRAIN=cv. Columbia, and cv. Wassilewskija;
RX PubMed=9108281; DOI=10.1007/s004380050407;
RA Ohshima S., Murata M., Sakamoto W., Ogura Y., Motoyoshi F.;
RT "Cloning and molecular analysis of the Arabidopsis gene Terminal Flower
RT 1.";
RL Mol. Gen. Genet. 254:186-194(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [6]
RP MUTAGENESIS OF HIS-88; LEU-113 AND PHE-123.
RX PubMed=15894619; DOI=10.1073/pnas.0500932102;
RA Hanzawa Y., Money T., Bradley D.;
RT "A single amino acid converts a repressor to an activator of flowering.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:7748-7753(2005).
CC -!- FUNCTION: Controls inflorescence meristem identity and is required for
CC maintenance of an indeterminate inflorescence. Prevents the expression
CC of 'APETALA1' and 'LEAFY'. Also plays a role in the regulation of the
CC time of flowering in the long-day flowering pathway. May form complexes
CC with phosphorylated ligands by interfering with kinases and their
CC effectors (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed below the apical dome of inflorescence
CC and coflorescence meristems, and in inflorescence stem.
CC -!- DEVELOPMENTAL STAGE: Weakly expressed in vegetative phase from day 2 or
CC day 3. Increased expression after commitment to flowering from day 7
CC on.
CC -!- MISCELLANEOUS: Mutagenesis of His-88 to Tyr converts the repressor of
CC flowering 'TERMINAL FLOWER 1' into a 'FLOWERING LOCUS T'-like activator
CC of flowering.
CC -!- SIMILARITY: Belongs to the phosphatidylethanolamine-binding protein
CC family. {ECO:0000305}.
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DR EMBL; U77674; AAB41624.1; -; mRNA.
DR EMBL; D86932; BAA20483.1; -; Genomic_DNA.
DR EMBL; D87130; BAA20484.1; -; Genomic_DNA.
DR EMBL; D87519; BAA20485.1; -; Genomic_DNA.
DR EMBL; AB005235; BAB08610.1; -; Genomic_DNA.
DR EMBL; AL162873; CAB85504.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90661.1; -; Genomic_DNA.
DR PIR; T48411; T48411.
DR RefSeq; NP_196004.1; NM_120465.3.
DR PDB; 1WKO; X-ray; 1.80 A; A/B=1-177.
DR PDBsum; 1WKO; -.
DR AlphaFoldDB; P93003; -.
DR SMR; P93003; -.
DR BioGRID; 16959; 2.
DR IntAct; P93003; 2.
DR STRING; 3702.AT5G03840.1; -.
DR PaxDb; P93003; -.
DR PRIDE; P93003; -.
DR ProteomicsDB; 234214; -.
DR EnsemblPlants; AT5G03840.1; AT5G03840.1; AT5G03840.
DR GeneID; 831683; -.
DR Gramene; AT5G03840.1; AT5G03840.1; AT5G03840.
DR KEGG; ath:AT5G03840; -.
DR Araport; AT5G03840; -.
DR TAIR; locus:2150595; AT5G03840.
DR eggNOG; KOG3346; Eukaryota.
DR HOGENOM; CLU_043994_6_1_1; -.
DR InParanoid; P93003; -.
DR OMA; FVFKQNE; -.
DR OrthoDB; 1557122at2759; -.
DR PhylomeDB; P93003; -.
DR EvolutionaryTrace; P93003; -.
DR PRO; PR:P93003; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; P93003; baseline and differential.
DR Genevisible; P93003; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0005773; C:vacuole; IDA:TAIR.
DR GO; GO:0031982; C:vesicle; IDA:TAIR.
DR GO; GO:0003712; F:transcription coregulator activity; IMP:TAIR.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0010022; P:meristem determinacy; IGI:TAIR.
DR GO; GO:0009910; P:negative regulation of flower development; IMP:TAIR.
DR GO; GO:0006623; P:protein targeting to vacuole; IMP:TAIR.
DR GO; GO:0009744; P:response to sucrose; IMP:TAIR.
DR GO; GO:0010228; P:vegetative to reproductive phase transition of meristem; IBA:GO_Central.
DR CDD; cd00866; PEBP_euk; 1.
DR Gene3D; 3.90.280.10; -; 1.
DR InterPro; IPR008914; PEBP.
DR InterPro; IPR036610; PEBP-like_sf.
DR InterPro; IPR035810; PEBP_euk.
DR InterPro; IPR001858; Phosphatidylethanolamine-bd_CS.
DR PANTHER; PTHR11362; PTHR11362; 1.
DR Pfam; PF01161; PBP; 1.
DR SUPFAM; SSF49777; SSF49777; 1.
DR PROSITE; PS01220; PBP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Developmental protein; Differentiation; Flowering;
KW Reference proteome.
FT CHAIN 1..177
FT /note="Protein TERMINAL FLOWER 1"
FT /id="PRO_0000204761"
FT MUTAGEN 69
FT /note="T->I: In tfl1-14; early-flowering and terminal
FT inflorescence."
FT MUTAGEN 87
FT /note="E->K: In tfl1-13; early-flowering and terminal
FT inflorescence."
FT MUTAGEN 88
FT /note="H->Y: Early-flowering and terminal inflorescence."
FT /evidence="ECO:0000269|PubMed:15894619"
FT MUTAGEN 98
FT /note="G->S: In tfl1-11; early-flowering and terminal
FT inflorescence."
FT MUTAGEN 105
FT /note="G->D: In tfl1-1; early-flowering and terminal
FT inflorescence."
FT MUTAGEN 113
FT /note="L->N: No effect on terminal flower formation."
FT /evidence="ECO:0000269|PubMed:15894619"
FT MUTAGEN 123
FT /note="F->V: Early-flowering and terminal inflorescence."
FT /evidence="ECO:0000269|PubMed:15894619"
FT HELIX 10..14
FT /evidence="ECO:0007829|PDB:1WKO"
FT HELIX 17..20
FT /evidence="ECO:0007829|PDB:1WKO"
FT STRAND 29..35
FT /evidence="ECO:0007829|PDB:1WKO"
FT HELIX 48..51
FT /evidence="ECO:0007829|PDB:1WKO"
FT STRAND 56..62
FT /evidence="ECO:0007829|PDB:1WKO"
FT STRAND 67..74
FT /evidence="ECO:0007829|PDB:1WKO"
FT STRAND 88..97
FT /evidence="ECO:0007829|PDB:1WKO"
FT HELIX 102..104
FT /evidence="ECO:0007829|PDB:1WKO"
FT STRAND 105..109
FT /evidence="ECO:0007829|PDB:1WKO"
FT STRAND 121..129
FT /evidence="ECO:0007829|PDB:1WKO"
FT HELIX 148..154
FT /evidence="ECO:0007829|PDB:1WKO"
FT STRAND 161..168
FT /evidence="ECO:0007829|PDB:1WKO"
SQ SEQUENCE 177 AA; 20158 MW; 02132C48E5F76BAC CRC64;
MENMGTRVIE PLIMGRVVGD VLDFFTPTTK MNVSYNKKQV SNGHELFPSS VSSKPRVEIH
GGDLRSFFTL VMIDPDVPGP SDPFLKEHLH WIVTNIPGTT DATFGKEVVS YELPRPSIGI
HRFVFVLFRQ KQRRVIFPNI PSRDHFNTRK FAVEYDLGLP VAAVFFNAQR ETAARKR
