ID   TFLIV_BPT5              Reviewed;         227 AA.
AC   Q6QGL2; Q5DMM4;
DT   17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=H-N-H endonuclease F-TflIV {ECO:0000312|EMBL:AAS77121.1};
DE            EC=3.1.21.- {ECO:0000305|PubMed:15456135};
DE   AltName: Full=HNH endodeoxyribonuclease F-TflIV;
DE   AltName: Full=HNH endonuclease F-TflIV {ECO:0000312|EMBL:AAU09414.1};
GN   Name=hegA {ECO:0000312|EMBL:AAS77121.1};
GN   ORFNames=ORF070 {ECO:0000312|EMBL:AAX12007.1},
GN   T5.075 {ECO:0000312|EMBL:AAS77121.1}, T5p073 {ECO:0000312|EMBL:AAU09414.1};
OS   Escherichia phage T5 (Enterobacteria phage T5).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Demerecviridae; Markadamsvirinae; Tequintavirus.
OX   NCBI_TaxID=2695836;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7533890;
RA   Shliapnikov M.G., Ksenzenko V.N.;
RT   "Features of the structure of transfer RNA coded by bacteriophage T5.";
RL   Mol. Biol. (Mosk.) 28:1321-1329(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Ksenzenko V.N., Kaliman A.V., Krutilina A.I., Shlyapnikov M.G.;
RT   "Bacteriophage T5 complete genome.";
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND INDUCTION.
RC   STRAIN=ATCC 11303-B5 {ECO:0000312|EMBL:AAX12007.1};
RX   PubMed=15661140; DOI=10.1016/j.virol.2004.10.049;
RA   Wang J., Jiang Y., Vincent M., Sun Y., Yu H., Wang J., Bao Q., Kong H.,
RA   Hu S.;
RT   "Complete genome sequence of bacteriophage T5.";
RL   Virology 332:45-65(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=St0 deletion mutant;
RX   PubMed=24198424; DOI=10.1128/jvi.02262-13;
RA   Zivanovic Y., Confalonieri F., Ponchon L., Lurz R., Chami M., Flayhan A.,
RA   Renouard M., Huet A., Decottignies P., Davidson A.R., Breyton C.,
RA   Boulanger P.;
RT   "Insights into bacteriophage T5 structure from analysis of its
RT   morphogenesis genes and protein components.";
RL   J. Virol. 88:1162-1174(2014).
RN   [5]
RP   FUNCTION.
RX   PubMed=15456135;
RA   Akulenko N.V., Ivashina T.V., Shaloiko L.A., Shliapnikov M.G.,
RA   Ksenzenko V.N.;
RT   "Novel site-specific endonucleases F-TflI, F-TflII and F-TflIV encoded by
RT   the bacteriophage T5.";
RL   Mol. Biol. (Mosk.) 38:632-641(2004).
CC   -!- FUNCTION: Endonuclease that introduces double-strand break into pseudo
CC       palindromic 17 bp DNA sequence yielding 1 bp extensions with 3'-
CC       overhangs. {ECO:0000269|PubMed:15456135}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000305|PubMed:15661140}.
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DR   EMBL; AY543070; AAS77121.1; -; Genomic_DNA.
DR   EMBL; AY587007; AAX12007.1; -; Genomic_DNA.
DR   EMBL; AY734508; AAU09414.1; -; Genomic_DNA.
DR   RefSeq; YP_006903.1; NC_005859.1.
DR   SMR; Q6QGL2; -.
DR   REBASE; 10523; F-EcoT5IV.
DR   GeneID; 2777587; -.
DR   KEGG; vg:2777587; -.
DR   Proteomes; UP000002107; Genome.
DR   Proteomes; UP000002141; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004519; F:endonuclease activity; IDA:UniProtKB.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00085; HNHc; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR003615; HNH_nuc.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00196; GerE; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Early protein; Endonuclease; Hydrolase; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..227
FT                   /note="H-N-H endonuclease F-TflIV"
FT                   /id="PRO_0000435549"
FT   CONFLICT        226..227
FT                   /note="NR -> T (in Ref. 3; AAX12007)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   227 AA;  25763 MW;  340D0682EF8DA71D CRC64;
     MRTDILDRKE EIAQWIAQGK SKAEIARMLS CSSNTLEAYL GKLGIVVIPT NRQYDNKYKS
     ATEYLYNGSP ISSYKLKNKI LNEGLKPHKC ESCGLESWLD KPIPLELEHK DGNHYNNEWD
     NLALLCPNCH ALTPTHAGKN IGRYTERTVN TCAICHCEIS SRATHCKSCT PKGITINPDI
     TVEQIEYWVS KYSWIRASKE LGLSDTGLRK RYKSLTGKDP KSIKKNR