ID   TFNPH_ASFM2             Reviewed;         706 AA.
AC   Q89926;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Termination factor NPH-I homolog {ECO:0000250|UniProtKB:Q89581};
DE            EC=3.6.4.- {ECO:0000250|UniProtKB:Q89581};
GN   OrderedLocusNames=Mal-130; ORFNames=j10L;
OS   African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10500;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=85517; Phacochoerus aethiopicus (Warthog).
OH   NCBI_TaxID=41426; Phacochoerus africanus (Warthog).
OH   NCBI_TaxID=273792; Potamochoerus larvatus (Bushpig).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8376971; DOI=10.1099/0022-1317-74-9-1969;
RA   Baylis S.A., Twigg S.R.F., Vydelingum S., Dixon L.K., Smith G.L.;
RT   "Three African swine fever virus genes encoding proteins with homology to
RT   putative helicases of vaccinia virus.";
RL   J. Gen. Virol. 74:1969-1974(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8397501; DOI=10.1007/bf01309543;
RA   Roberts P.C., Lu Z., Kutish G.F., Rock D.L.;
RT   "Three adjacent genes of African swine fever virus with similarity to
RT   essential poxvirus genes.";
RL   Arch. Virol. 132:331-342(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8021596; DOI=10.1099/0022-1317-75-7-1655;
RA   Dixon L.K., Twigg S.R.F., Baylis S.A., Vydelingum S., Bristow C.,
RA   Hammond J.M., Smith G.L.;
RT   "Nucleotide sequence of a 55 kbp region from the right end of the genome of
RT   a pathogenic African swine fever virus isolate (Malawi LIL20/1).";
RL   J. Gen. Virol. 75:1655-1684(1994).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Kutish G.F., Rock D.L.;
RT   "African swine fever virus genomes.";
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Putative DNA-dependent ATPase required for providing the
CC       needed energy to achieve the termination of early transcripts.
CC       {ECO:0000250|UniProtKB:P05807}.
CC   -!- SUBUNIT: Part of the viral DNA-directed RNA polymerase that consists of
CC       8 polII-like subunits (RPB1, RPB2, RPB3, RPB5, RPB6, RPB7, RPB9,
CC       RPB10), a capping enzyme and a termination factor.
CC       {ECO:0000250|UniProtKB:Q89581}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250|UniProtKB:Q89581}. Note=Found
CC       in association with viral nucleoid. {ECO:0000250|UniProtKB:Q89581}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC       {ECO:0000305}.
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DR   EMBL; X72952; CAA51457.1; -; Genomic_DNA.
DR   EMBL; M88275; AAA03222.1; -; Genomic_DNA.
DR   EMBL; X71982; CAA50829.1; -; Genomic_DNA.
DR   EMBL; AY261361; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; JQ2210; JQ2210.
DR   Proteomes; UP000000860; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0140658; F:ATP-dependent chromatin remodeler activity; IEA:InterPro.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.10810; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR038718; SNF2-like_sf.
DR   InterPro; IPR000330; SNF2_N.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF00176; SNF2-rel_dom; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Virion.
FT   CHAIN           1..706
FT                   /note="Termination factor NPH-I homolog"
FT                   /id="PRO_0000373118"
FT   DOMAIN          62..227
FT                   /note="Helicase ATP-binding"
FT   DOMAIN          417..599
FT                   /note="Helicase C-terminal"
FT   MOTIF           168..171
FT                   /note="DEAH box"
FT   BINDING         75..82
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   706 AA;  80499 MW;  0D22C089781FAA46 CRC64;
     MSCVHNNTSF PVQIEAYLKE VFEKYKELQE SKDTSLTARF ARVLKYYQFL IYSAFSDPKF
     GIGQGENTRG LLIYHQMGMG KTILSLSLAI SLSHIYNPIL IAPKSLHSNF QQSLLKLIKL
     LYPETTDHSK ELQKISRRFR FVSLDAYNMG QQIIKAGGSL NGCLLIVDEA HNLFRGIINS
     ANDKTNARQL YNNIMQAKNI RILFLTGTPC SKDPFEMVPC FNMLSGRILL PLHYERFYTA
     YVNKTTNSPL NADKLLNRLV GMISYAGNQN ELNKLFPTEL PLIIEKVEMS PEQYRQYLLA
     RDVENAEKHA SSGMYEKMNT AALCLPGSEQ ESGSSYYVRS RMISIFASEM LTVKEDEKLS
     EAVQQLPKEA FTENSSPKIA CMLKNIKTSP GPVLIYSQFV ELGLHVVARF LEIEGYQCLQ
     PLKVLEEGHN TILLHKDGKD LMVKNFAEDE PTHTLVLSSK ITRFTLITGK ILSKERDMIQ
     QLWNSPLNIH GEVIKILLVS KTGAEGLDLK YGRQVHILEP YWDKAREDQV KARIIRIGSH
     DALPPEEKTV QPFLYIAVAN QKMFYSIPEG SQEQKTIDER FHERGLEKSH LNSAFRDLLK
     RAAIECAFNG ESGCLMCQPT NALLFHENFE RDLRLPNPCQ PLVKTEVKAY SISYEGKQFF
     YQKNKEVGLG YTFYEYNPII KAYIEIKPSN PLYIKLIKHV QAGTTV