TFOX_HAEIN
ID TFOX_HAEIN Reviewed; 217 AA.
AC P43779;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=DNA transformation protein TfoX;
DE AltName: Full=Competence activator Sxy {ECO:0000303|PubMed:23663205};
DE AltName: Full=Protein Sxy;
GN Name=tfoX; Synonyms=sxy; OrderedLocusNames=HI_0601;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7724607; DOI=10.1073/pnas.92.8.3616;
RA Zulty J.J., Barcak G.J.;
RT "Identification of a DNA transformation gene required for com101A+
RT expression and supertransformer phenotype in Haemophilus influenzae.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:3616-3620(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
RN [3]
RP FUNCTION.
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd {ECO:0000303|PubMed:1653215};
RX PubMed=1653215; DOI=10.1128/jb.173.18.5612-5618.1991;
RA Redfield R.J.;
RT "sxy-1, a Haemophilus influenzae mutation causing greatly enhanced
RT spontaneous competence.";
RL J. Bacteriol. 173:5612-5618(1991).
RN [4]
RP IDENTIFICATION OF TFOX AS SXY, AND VARIANT SXY-1.
RX PubMed=7961436; DOI=10.1128/jb.176.22.6789-6794.1994;
RA Williams P.M., Bannister L.A., Redfield R.J.;
RT "The Haemophilus influenzae sxy-1 mutation is in a newly identified gene
RT essential for competence.";
RL J. Bacteriol. 176:6789-6794(1994).
RN [5]
RP FUNCTION.
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd {ECO:0000303|PubMed:9244270};
RX PubMed=9244270; DOI=10.1128/jb.179.15.4815-4820.1997;
RA Karudapuram S., Barcak G.J.;
RT "The Haemophilus influenzae dprABC genes constitute a competence-inducible
RT operon that requires the product of the tfoX (sxy) gene for transcriptional
RT activation.";
RL J. Bacteriol. 179:4815-4820(1997).
RN [6]
RP INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=15769466; DOI=10.1016/j.jmb.2005.01.012;
RA Redfield R.J., Cameron A.D., Qian Q., Hinds J., Ali T.R., Kroll J.S.,
RA Langford P.R.;
RT "A novel CRP-dependent regulon controls expression of competence genes in
RT Haemophilus influenzae.";
RL J. Mol. Biol. 347:735-747(2005).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=18761017; DOI=10.1016/j.jmb.2008.08.027;
RA Cameron A.D., Redfield R.J.;
RT "CRP binding and transcription activation at CRP-S sites.";
RL J. Mol. Biol. 383:313-323(2008).
RN [8]
RP INDUCTION.
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd {ECO:0000303|PubMed:17981840};
RX PubMed=17981840; DOI=10.1093/nar/gkm915;
RA Cameron A.D., Volar M., Bannister L.A., Redfield R.J.;
RT "RNA secondary structure regulates the translation of sxy and competence
RT development in Haemophilus influenzae.";
RL Nucleic Acids Res. 36:10-20(2008).
RN [9]
RP INDUCTION.
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd {ECO:0000303|PubMed:23663205};
RX PubMed=23663205; DOI=10.1111/mmi.12245;
RA Sinha S., Mell J., Redfield R.;
RT "The availability of purine nucleotides regulates natural competence by
RT controlling translation of the competence activator Sxy.";
RL Mol. Microbiol. 88:1106-1119(2013).
CC -!- FUNCTION: Required for DNA transformation (PubMed:1653215,
CC PubMed:18761017). Positively regulates genes required for DNA
CC transformation (late competence-specific genes) in association with CRP
CC (PubMed:18761017). Required for expression of the dprABC operon
CC (PubMed:9244270). {ECO:0000269|PubMed:1653215,
CC ECO:0000269|PubMed:18761017, ECO:0000269|PubMed:9244270}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=Long;
CC IsoId=P43779-1; Sequence=Displayed;
CC Name=Short;
CC IsoId=P43779-2; Sequence=VSP_018799;
CC -!- INDUCTION: Induced about 50-fold on moving from rich to starvation
CC medium. By CRP and cAMP in starvation medium. Translation increased by
CC depletion of extra- or intra-cellular purine nucleotides in starvation
CC medium. High levels of purine nucleotides by contrast decrease its
CC translation under the same condition. {ECO:0000269|PubMed:15769466,
CC ECO:0000269|PubMed:17981840, ECO:0000269|PubMed:23663205}.
CC -!- DISRUPTION PHENOTYPE: Loss of expression of the competence regulatory
CC element (CRE) regulon required for development of competence.
CC {ECO:0000269|PubMed:15769466, ECO:0000269|PubMed:18761017}.
CC -!- SIMILARITY: Belongs to the TfoX family. {ECO:0000305}.
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DR EMBL; U13205; AAC43320.1; -; Genomic_DNA.
DR EMBL; U13205; AAC43321.1; -; Genomic_DNA.
DR EMBL; L42023; AAC22258.1; -; Genomic_DNA.
DR PIR; B64080; B64080.
DR RefSeq; NP_438758.1; NC_000907.1.
DR RefSeq; WP_010869017.1; NC_000907.1.
DR AlphaFoldDB; P43779; -.
DR SMR; P43779; -.
DR STRING; 71421.HI_0601; -.
DR EnsemblBacteria; AAC22258; AAC22258; HI_0601.
DR KEGG; hin:HI_0601; -.
DR PATRIC; fig|71421.8.peg.622; -.
DR eggNOG; COG3070; Bacteria.
DR HOGENOM; CLU_094990_2_0_6; -.
DR OMA; AICGTHQ; -.
DR PhylomeDB; P43779; -.
DR BioCyc; HINF71421:G1GJ1-611-MON; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0071230; P:cellular response to amino acid stimulus; IMP:UniProtKB.
DR GO; GO:0071320; P:cellular response to cAMP; IMP:UniProtKB.
DR GO; GO:0071415; P:cellular response to purine-containing compound; IEP:UniProtKB.
DR GO; GO:0009267; P:cellular response to starvation; IMP:UniProtKB.
DR GO; GO:0009290; P:DNA import into cell involved in transformation; IMP:UniProtKB.
DR GO; GO:0030420; P:establishment of competence for transformation; IMP:UniProtKB.
DR GO; GO:0045809; P:positive regulation of establishment of competence for transformation; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR InterPro; IPR007077; TfoX_C.
DR InterPro; IPR007076; TfoX_N.
DR InterPro; IPR026256; TfoX_Sxy.
DR Pfam; PF04994; TfoX_C; 1.
DR Pfam; PF04993; TfoX_N; 1.
DR PIRSF; PIRSF028788; TfoX_Sxy; 1.
PE 2: Evidence at transcript level;
KW Activator; Alternative initiation; Competence; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..217
FT /note="DNA transformation protein TfoX"
FT /id="PRO_0000022483"
FT VAR_SEQ 1..39
FT /note="Missing (in isoform Short)"
FT /evidence="ECO:0000305"
FT /id="VSP_018799"
FT VARIANT 19
FT /note="V -> I (in sxy-Long; causes a 100-fold to 1000-fold
FT increase in spontaneous natural competence)"
FT CONFLICT 203
FT /note="W -> L (in Ref. 1; AAC43320/AAC43321)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 217 AA; 25074 MW; 4A6E30E64008CC3F CRC64;
MNIKDEHIDS VCSLLDQLVG NVSFKNLFTG YGLFHKEETM FAIWQNKKLY LRGEGVLAIQ
LTKLGCEPFT TNELNKRFVL SQYYALSDQI LRSNRLCRKL IILSIKQILE QKLECTLRKL
NRLKDLPNLT IKHERALIKV GITNVAMLRE IGAENALVEL KKSGSGATLD FYWKLVCALQ
NKNSQMLSQA EKERLLKKLN EVWRKNGLKG YRKLDDE
