TFP11_DROME
ID TFP11_DROME Reviewed; 839 AA.
AC Q9Y103; Q9NHN7;
DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Septin-interacting protein 1;
DE AltName: Full=Septin and tuftelin-interacting protein 1;
DE Short=STIP-1;
GN Name=sip1; Synonyms=stip-1; ORFNames=CG7238;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INTERACTION WITH PNUT.
RX PubMed=11884525; DOI=10.1242/jcs.115.6.1259;
RA Shih H.-P., Hales K.G., Pringle J.R., Peifer M.;
RT "Identification of septin-interacting proteins and characterization of the
RT Smt3/SUMO-conjugation system in Drosophila.";
RL J. Cell Sci. 115:1259-1271(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43; SER-47 AND THR-53, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: May be involved in pre-mRNA splicing. {ECO:0000250}.
CC -!- SUBUNIT: Identified in the spliceosome C complex (By similarity).
CC Interacts with pnut. {ECO:0000250, ECO:0000269|PubMed:11884525}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TFP11/STIP family. {ECO:0000305}.
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DR EMBL; AF221101; AAF26743.1; -; mRNA.
DR EMBL; AE014134; AAF52282.1; -; Genomic_DNA.
DR EMBL; AF145670; AAD38645.1; -; mRNA.
DR RefSeq; NP_001285636.1; NM_001298707.1.
DR RefSeq; NP_524725.2; NM_079986.4.
DR AlphaFoldDB; Q9Y103; -.
DR SMR; Q9Y103; -.
DR BioGRID; 68911; 47.
DR IntAct; Q9Y103; 1.
DR STRING; 7227.FBpp0078765; -.
DR iPTMnet; Q9Y103; -.
DR PaxDb; Q9Y103; -.
DR PRIDE; Q9Y103; -.
DR EnsemblMetazoa; FBtr0079134; FBpp0078765; FBgn0024191.
DR EnsemblMetazoa; FBtr0345545; FBpp0311644; FBgn0024191.
DR GeneID; 44238; -.
DR KEGG; dme:Dmel_CG7238; -.
DR CTD; 44238; -.
DR FlyBase; FBgn0024191; sip1.
DR VEuPathDB; VectorBase:FBgn0024191; -.
DR eggNOG; KOG2184; Eukaryota.
DR HOGENOM; CLU_007977_1_1_1; -.
DR InParanoid; Q9Y103; -.
DR OMA; EFFPKWH; -.
DR OrthoDB; 1238995at2759; -.
DR PhylomeDB; Q9Y103; -.
DR SignaLink; Q9Y103; -.
DR BioGRID-ORCS; 44238; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 44238; -.
DR PRO; PR:Q9Y103; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0024191; Expressed in eye disc (Drosophila) and 20 other tissues.
DR ExpressionAtlas; Q9Y103; baseline and differential.
DR Genevisible; Q9Y103; DM.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; HDA:FlyBase.
DR GO; GO:0005737; C:cytoplasm; HDA:FlyBase.
DR GO; GO:0071011; C:precatalytic spliceosome; HDA:FlyBase.
DR GO; GO:0005681; C:spliceosomal complex; ISS:UniProtKB.
DR GO; GO:0071008; C:U2-type post-mRNA release spliceosomal complex; IBA:GO_Central.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IC:FlyBase.
DR GO; GO:0000390; P:spliceosomal complex disassembly; IBA:GO_Central.
DR InterPro; IPR000467; G_patch_dom.
DR InterPro; IPR022783; GCFC_dom.
DR InterPro; IPR024933; STIP.
DR InterPro; IPR022159; STIP/TFIP11_N.
DR InterPro; IPR045211; TFP11/STIP/Ntr1.
DR PANTHER; PTHR23329; PTHR23329; 1.
DR Pfam; PF01585; G-patch; 1.
DR Pfam; PF07842; GCFC; 1.
DR Pfam; PF12457; TIP_N; 1.
DR PIRSF; PIRSF017706; TFIP11; 1.
DR SMART; SM00443; G_patch; 1.
DR PROSITE; PS50174; G_PATCH; 1.
PE 1: Evidence at protein level;
KW Coiled coil; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein;
KW Reference proteome; Spliceosome.
FT CHAIN 1..839
FT /note="Septin-interacting protein 1"
FT /id="PRO_0000342284"
FT DOMAIN 167..213
FT /note="G-patch"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00092"
FT REGION 22..164
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 363..411
FT /evidence="ECO:0000255"
FT COMPBIAS 84..103
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 43
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 47
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 53
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CONFLICT 645
FT /note="N -> I (in Ref. 1; AAF26743)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 839 AA; 94828 MW; F2EF494F665A757C CRC64;
MSDNDYERFE ITDYDLDNEF NINRPRGRQS RHQQIYGIWA DDSEEESGGE GGTKRRGRAA
RKPKDYTMPV NFVAGGIQQA GKKKKKALQA DDEKGSQKEG AEADQGEESD DSAASGRPAF
GQNDPGSSNS SSEEERPTLS RKQPSTTFQH RSHIASERNV GAWEQHTRGI GAKLLLQMGY
EPGKGLGKDL QGISHPVQAH VRKGRGAIGA YGPETAASIG GKTNKSIKVD EDVREAKEFK
DQLNKWRKGS AGGAEPMERQ GKRYYYKSVE EVIAKGHTSG HLLSEKLSKK LGNVRVIDMT
GPEKRVLSGY HALGQAKITP EETLYDTEAT EKGSAPACVF AMPELTHNLQ LLVSQCEQQI
IAIDNQEREC SSQQAALESE HRKLEEIVQL ERNHIRTLEE SLERVERLID NPDLSLPQAE
RLFRELLVDY AAEFHEFGLA DLAAGVIAPL LKRELVQWQP LENPTEPLPL IKKWRGMLQQ
GDAAEQQPRN VFDPYSSLIW AGVMPSFRSS AAAWQPKEHP PMASLLDAWA PLLPSWVLDS
VLEQLVLPRL VAGVQEWDPL TDTVPIDSWV LPWHAILGSK LEEAVYPQIR SKLGIALRAW
SPHDRSARAM LTPWQKAFPE EEMQEFLQRY IVPKLQATLG ELIINPMHQD LELWQQVWEW
HELIDPMYMA QLLDRHFFPR WMQVLVVWLN QSPDYAEISR WYTGWKSMLS EPLLREPSVK
EHLRRALEIM HRASDTLLQP TVTPTPPPPV PPAPVIMMDL IHPPAQLEFK ELVSQQCADL
GIIFAPLPGR REMGKQIYRV GKLFCYIDRH VCMVSDGSFS NWKPVSLNHL LERSQTGIL
