ID   TG124_CAEEL             Reviewed;         402 AA.
AC   Q09524;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Probable tRNA pseudouridine synthase tag-124;
DE            EC=5.4.99.-;
DE   AltName: Full=tRNA pseudouridylate synthase;
DE   AltName: Full=tRNA-uridine isomerase;
GN   Name=tag-124; ORFNames=E02H1.3;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Formation of pseudouridine at position 38 and 39 in the
CC       anticodon stem and loop of transfer RNAs. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in tRNA = a pseudouridine in tRNA;
CC         Xref=Rhea:RHEA:54572, Rhea:RHEA-COMP:13339, Rhea:RHEA-COMP:13934,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z47075; CAA87378.1; -; Genomic_DNA.
DR   PIR; T20413; T20413.
DR   RefSeq; NP_496062.3; NM_063661.7.
DR   AlphaFoldDB; Q09524; -.
DR   SMR; Q09524; -.
DR   STRING; 6239.E02H1.3; -.
DR   EPD; Q09524; -.
DR   PaxDb; Q09524; -.
DR   PeptideAtlas; Q09524; -.
DR   EnsemblMetazoa; E02H1.3.1; E02H1.3.1; WBGene00006473.
DR   GeneID; 174509; -.
DR   KEGG; cel:CELE_E02H1.3; -.
DR   UCSC; E02H1.3.1; c. elegans.
DR   CTD; 174509; -.
DR   WormBase; E02H1.3; CE01538; WBGene00006473; tag-124.
DR   eggNOG; KOG2554; Eukaryota.
DR   GeneTree; ENSGT00950000183160; -.
DR   HOGENOM; CLU_014673_2_0_1; -.
DR   InParanoid; Q09524; -.
DR   OMA; NLFRCDF; -.
DR   OrthoDB; 1093493at2759; -.
DR   PhylomeDB; Q09524; -.
DR   PRO; PR:Q09524; -.
DR   Proteomes; UP000001940; Chromosome II.
DR   Bgee; WBGene00006473; Expressed in germ line (C elegans) and 4 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:1990481; P:mRNA pseudouridine synthesis; IBA:GO_Central.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IBA:GO_Central.
DR   CDD; cd02569; PseudoU_synth_ScPus3; 1.
DR   Gene3D; 3.30.70.580; -; 1.
DR   Gene3D; 3.30.70.660; -; 1.
DR   HAMAP; MF_00171; TruA; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR001406; PsdUridine_synth_TruA.
DR   InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR   InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR   InterPro; IPR041707; Pus3-like.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   PANTHER; PTHR11142; PTHR11142; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00071; hisT_truA; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; tRNA processing.
FT   CHAIN           1..402
FT                   /note="Probable tRNA pseudouridine synthase tag-124"
FT                   /id="PRO_0000057523"
FT   REGION          383..402
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        85
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   402 AA;  46291 MW;  E2E6D61489BC85A5 CRC64;
     MGSKRVCPDA NNSVKSKKAK TLDFLAHPRR KIAIQFFYLG WEHDGLVQQP HTQNTVENHI
     MQALIKTHLI EDWTKCDFSR CGRTDKGVSA FKQTAAMVVR SLCPGDSGVF WSDSTQEHQK
     VDYKASGEEL PYVKMLNGVL PKTIRVFAWA PVAQTFNARF DCNRRTYKYS FAKADLNLEK
     MRQGAELLVG EHDFSNFCQI DMNEKRLLQS YVRKVYEVKV EQVSTHPEND MYSMVELTVS
     GSGFLWHMIR YIVTILQEIG RENEQPSLIS QLLDLKKYPS RPQYTLASDT PLCLFDCGYK
     SEDVEWKVHD YTLKSTVTGL QKTWATYQAR SRMMENMLGE LTGMAEFSSG DANKGLHEFV
     QDRPIPSNYI RFENRKMCES LESKKEKMAE KKKNGEESSD KL