TGA21_ORYSJ
ID TGA21_ORYSJ Reviewed; 334 AA.
AC Q7X993; Q8W5R9;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Transcription factor TGA2.1 {ECO:0000305};
DE AltName: Full=OsNIF2 {ECO:0000312|EMBL:BAB72062.1};
DE AltName: Full=bZIP transcription factor 63 {ECO:0000303|PubMed:18065552};
DE Short=OsbZIP63 {ECO:0000303|PubMed:18065552};
GN Name=TGA2.1 {ECO:0000303|PubMed:24919709};
GN OrderedLocusNames=Os07g0687700 {ECO:0000312|EMBL:BAF22609.1},
GN LOC_Os07g48820 {ECO:0000305};
GN ORFNames=OJ1150_E04.124 {ECO:0000312|EMBL:BAD30297.1},
GN OJ1165_F02.101 {ECO:0000312|EMBL:BAC79596.1},
GN OsJ_25648 {ECO:0000312|EMBL:EEE67849.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=18987217; DOI=10.1104/pp.108.128579;
RA Yilmaz A., Nishiyama M.Y., Fuentes B.G., Souza G.M., Janies D., Gray J.,
RA Grotewold E.;
RT "GRASSIUS: a platform for comparative regulatory genomics across the
RT grasses.";
RL Plant Physiol. 149:171-180(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Yokoyama T., Motoyama T., Yoneyama K., Yamaguchi I.;
RT "cDNA cloning of bZIP transcription factors from rice.";
RL Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [8]
RP FUNCTION, AND MUTAGENESIS OF 61-ALA-ALA-62.
RX PubMed=16045470; DOI=10.1111/j.1365-313x.2005.02457.x;
RA Fitzgerald H.A., Canlas P.E., Chern M.S., Ronald P.C.;
RT "Alteration of TGA factor activity in rice results in enhanced tolerance to
RT Xanthomonas oryzae pv. oryzae.";
RL Plant J. 43:335-347(2005).
RN [9]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18065552; DOI=10.1104/pp.107.112821;
RA Nijhawan A., Jain M., Tyagi A.K., Khurana J.P.;
RT "Genomic survey and gene expression analysis of the basic leucine zipper
RT transcription factor family in rice.";
RL Plant Physiol. 146:333-350(2008).
RN [10]
RP FUNCTION.
RX PubMed=22353606; DOI=10.1186/1746-4811-8-6;
RA Chern M., Bai W., Sze-To W.H., Canlas P.E., Bartley L.E., Ronald P.C.;
RT "A rice transient assay system identifies a novel domain in NRR required
RT for interaction with NH1/OsNPR1 and inhibition of NH1-mediated
RT transcriptional activation.";
RL Plant Methods 8:6-6(2012).
RN [11]
RP INTERACTION WITH NPR1/NH1 AND NPR3/NH3.
RX PubMed=24919709; DOI=10.1186/1471-2164-15-461;
RA Chern M., Bai W., Ruan D., Oh T., Chen X., Ronald P.C.;
RT "Interaction specificity and coexpression of rice NPR1 homologs 1 and 3
RT (NH1 and NH3), TGA transcription factors and negative regulator of
RT resistance (NRR) proteins.";
RL BMC Genomics 15:461-461(2014).
RN [12]
RP FUNCTION.
RX PubMed=27176732; DOI=10.1371/journal.pgen.1006049;
RA Chern M., Xu Q., Bart R.S., Bai W., Ruan D., Sze-To W.H., Canlas P.E.,
RA Jain R., Chen X., Ronald P.C.;
RT "A genetic screen identifies a requirement for cysteine-rich-receptor-like
RT kinases in rice NH1 (OsNPR1)-mediated immunity.";
RL PLoS Genet. 12:E1006049-E1006049(2016).
CC -!- FUNCTION: Plays a negative role in rice basal defense responses to the
CC bacterial blight pathogen Xanthomomas oryzae pv. oryzae (Xoo). May
CC function in both positive and negative regulation of rice defense
CC genes. Binds DNA in vitro (PubMed:16045470). Acts as transcriptional
CC activator when bound to NPR1/NH1 in vitro (PubMed:22353606). Binds to
CC the promoter sequence of CRK10 in vitro (PubMed:27176732).
CC {ECO:0000269|PubMed:16045470, ECO:0000269|PubMed:22353606,
CC ECO:0000269|PubMed:27176732}.
CC -!- SUBUNIT: Interacts with NPR1/NH1 and NPR3/NH3.
CC {ECO:0000269|PubMed:24919709}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00978}.
CC -!- MISCELLANEOUS: Plants silencing TGA2.1 display increased tolerance to
CC the bacterial blight pathogen Xanthomomas oryzae pv. oryzae, are dwarf,
CC and present altered expression of pathogenesis-related (PR) genes.
CC {ECO:0000269|PubMed:16045470}.
CC -!- SIMILARITY: Belongs to the bZIP family. {ECO:0000305}.
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DR EMBL; HQ858807; ADX60219.1; -; mRNA.
DR EMBL; AB051295; BAB72062.1; -; mRNA.
DR EMBL; AP003813; BAD30297.1; -; Genomic_DNA.
DR EMBL; AP003816; BAC79596.1; -; Genomic_DNA.
DR EMBL; AP008213; BAF22609.1; -; Genomic_DNA.
DR EMBL; AP014963; BAT03308.1; -; Genomic_DNA.
DR EMBL; CM000144; EEE67849.1; -; Genomic_DNA.
DR EMBL; AK100440; BAG94609.1; -; mRNA.
DR RefSeq; XP_015647505.1; XM_015792019.1.
DR RefSeq; XP_015647506.1; XM_015792020.1.
DR AlphaFoldDB; Q7X993; -.
DR SMR; Q7X993; -.
DR STRING; 4530.OS07T0687700-01; -.
DR PaxDb; Q7X993; -.
DR PRIDE; Q7X993; -.
DR EnsemblPlants; Os07t0687700-01; Os07t0687700-01; Os07g0687700.
DR GeneID; 4344349; -.
DR Gramene; Os07t0687700-01; Os07t0687700-01; Os07g0687700.
DR KEGG; osa:4344349; -.
DR eggNOG; ENOG502QU32; Eukaryota.
DR HOGENOM; CLU_024782_1_1_1; -.
DR InParanoid; Q7X993; -.
DR OMA; MDQKTMR; -.
DR OrthoDB; 1018848at2759; -.
DR PlantReactome; R-OSA-6788019; Salicylic acid signaling.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000007752; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0042742; P:defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR InterPro; IPR004827; bZIP.
DR InterPro; IPR046347; bZIP_sf.
DR InterPro; IPR025422; TGA_domain.
DR Pfam; PF00170; bZIP_1; 1.
DR Pfam; PF14144; DOG1; 1.
DR SMART; SM00338; BRLZ; 1.
DR SUPFAM; SSF57959; SSF57959; 1.
DR PROSITE; PS50217; BZIP; 1.
DR PROSITE; PS00036; BZIP_BASIC; 1.
DR PROSITE; PS51806; DOG1; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Nucleus; Plant defense; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..334
FT /note="Transcription factor TGA2.1"
FT /id="PRO_0000437012"
FT DOMAIN 48..92
FT /note="bZIP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT DOMAIN 115..331
FT /note="DOG1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01147"
FT REGION 1..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 50..70
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 76..90
FT /note="Leucine-zipper"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT MUTAGEN 61..62
FT /note="AA->PP: Loss of DNA binding capacity."
FT /evidence="ECO:0000269|PubMed:16045470"
SQ SEQUENCE 334 AA; 37215 MW; 9402D79C72891534 CRC64;
MADASSRTDT SIVVDNDDKN HQLENGHSGA VMASNSSDRS DRSDKLMDQK TIRRLAQNRE
AARKSRLRKK AYVQQLESSK LKLAQLEQEL QKARQQGIFI SSSGDQTHAM SGNGALTFDL
EYTRWLEEQN KQINELRTAV NAHASDSDLR LIVDGIMAHY DEVFKVKGVA AKADVFHILS
GMWKTPAERC FLWLGGFRPS ELLKLLANHL EPLTEQQLLG LNNLQESSQQ AEDALSQGME
ALQQSLADTL AGSLGSSGSS GNVANYMGQM AMAMGKLGTL ENFLCQADNL RQQTLHQMQR
ILTIRQASRA LLAIHDYFSR LRALSSLWLA RPRE
