ID   TGA21_TOBAC             Reviewed;         456 AA.
AC   O24160;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=TGACG-sequence-specific DNA-binding protein TGA-2.1;
DE            Short=TGA2.1;
GN   Name=TGA21;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. SR1; TISSUE=Leaf;
RA   Niggeweg R., Gatz C.;
RT   "Isolation of TGA2.1, a member of a new subclass of the TGA family of bZIP
RT   transcription factors in Nicotiana tabacum.";
RL   (er) Plant Gene Register PGR97-060(1997).
RN   [2]
RP   CHARACTERIZATION.
RX   PubMed=10809449; DOI=10.1023/a:1006319113205;
RA   Niggeweg R., Thurow C., Weigel R., Pfitzner U., Gatz C.;
RT   "Tobacco TGA factors differ with respect to interaction with NPR1,
RT   activation potential and DNA-binding properties.";
RL   Plant Mol. Biol. 42:775-788(2000).
CC   -!- FUNCTION: Transcriptional activator that binds specifically to the DNA
CC       sequence 5'-TGACG-3'. Recognizes ocs elements like the as-1 motif of
CC       the cauliflower mosaic virus 35S promoter. Binding to the as-1-like cis
CC       elements mediate auxin- and salicylic acid-inducible transcription.
CC   -!- SUBUNIT: Can form heterodimer with TGA2.2.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00978}.
CC   -!- SIMILARITY: Belongs to the bZIP family. {ECO:0000305}.
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DR   EMBL; U90214; AAB68661.1; -; mRNA.
DR   PIR; T03935; T03935.
DR   RefSeq; NP_001312268.1; NM_001325339.1.
DR   AlphaFoldDB; O24160; -.
DR   IntAct; O24160; 1.
DR   STRING; 4097.O24160; -.
DR   GeneID; 107782457; -.
DR   KEGG; nta:107782457; -.
DR   OMA; TSRMSEF; -.
DR   PhylomeDB; O24160; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR004827; bZIP.
DR   InterPro; IPR046347; bZIP_sf.
DR   InterPro; IPR025422; TGA_domain.
DR   Pfam; PF00170; bZIP_1; 1.
DR   Pfam; PF14144; DOG1; 1.
DR   SMART; SM00338; BRLZ; 1.
DR   SUPFAM; SSF57959; SSF57959; 1.
DR   PROSITE; PS50217; BZIP; 1.
DR   PROSITE; PS00036; BZIP_BASIC; 1.
DR   PROSITE; PS51806; DOG1; 1.
PE   1: Evidence at protein level;
KW   Activator; Coiled coil; DNA-binding; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..456
FT                   /note="TGACG-sequence-specific DNA-binding protein TGA-2.1"
FT                   /id="PRO_0000076548"
FT   DOMAIN          166..229
FT                   /note="bZIP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT   DOMAIN          233..450
FT                   /note="DOG1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01147"
FT   REGION          1..41
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          115..170
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          168..188
FT                   /note="Basic motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT   REGION          194..208
FT                   /note="Leucine-zipper"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT   COILED          167..220
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        115..137
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        156..170
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   456 AA;  50082 MW;  E4768228210B8167 CRC64;
     MASKIGTAGN RSGTTGMPSF ISQIPVSNPM GTEANNTNTS RMSDFGVLEQ YLGFRIGDGA
     NVNRSPLFNS TSATNPAVGF EVSGTINRTL APSNTSLPTA TPRSQTMLLQ SNLVSASGTH
     HENWGESNMA DSGSRTDTST DMDGDDKNQL IEAGQSSDKS KEKVLDQKTL RRLAQNREAA
     RKSRLRKKAY VQQLENSRLK LSQLEQDLQR ARQQGKYISN IADQSNGVGA NGPLAFDAEY
     SRWLEEHNKH INELRTAVNA HASDPELRSI VNNVTAHFDE VFRVKGNAAK ADVFHVLSGM
     WKTPAERCFM WIGGFRPSEL LKLLVNQLEP LTEQQLAGIY NLQQSSHQAE DALSQGMEAL
     QQSLAETLAN GSPAPEGSSG DVANYMGQMA MAMGKLGTLE GFLRQADNLR QQTLQQMHRV
     LTTRQSARAL LAINEYFSRL RALSSLWLAR PREQLV