TGA3_ARATH
ID TGA3_ARATH Reviewed; 384 AA.
AC Q39234; Q8LBG4; Q9LM52;
DT 01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Transcription factor TGA3;
DE AltName: Full=bZIP transcription factor 22;
DE Short=AtbZIP22;
GN Name=TGA3; Synonyms=BZIP22; OrderedLocusNames=At1g22070; ORFNames=F2E2.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=8003690; DOI=10.1007/bf00024193;
RA Miao Z.-H., Liu X., Lam E.;
RT "TGA3 is a distinct member of the TGA family of bZIP transcription factors
RT in Arabidopsis thaliana.";
RL Plant Mol. Biol. 25:1-11(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP DNA-BINDING.
RX PubMed=7479010; DOI=10.1093/nar/23.18.3778;
RA Lam E., Lam Y.K.;
RT "Binding site requirements and differential representation of TGF factors
RT in nuclear ASF-1 activity.";
RL Nucleic Acids Res. 23:3778-3785(1995).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=8628224; DOI=10.1007/bf02174184;
RA de Pater S., Pham K., Memelink J., Kijne J.;
RT "Binding specificity and tissue-specific expression pattern of the
RT Arabidopsis bZIP transcription factor TGA2.";
RL Mol. Gen. Genet. 250:237-239(1996).
RN [7]
RP INTERACTION WITH NPR1.
RX PubMed=10659709; DOI=10.1094/mpmi.2000.13.2.191;
RA Zhou J.-M., Trifa Y., Silva H., Pontier D., Lam E., Shah J., Klessig D.F.;
RT "NPR1 differentially interacts with members of the TGA/OBF family of
RT transcription factors that bind an element of the PR-1 gene required for
RT induction by salicylic acid.";
RL Mol. Plant Microbe Interact. 13:191-202(2000).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11906833; DOI=10.1016/s1360-1385(01)02223-3;
RA Jakoby M., Weisshaar B., Droege-Laser W., Vicente-Carbajosa J.,
RA Tiedemann J., Kroj T., Parcy F.;
RT "bZIP transcription factors in Arabidopsis.";
RL Trends Plant Sci. 7:106-111(2002).
RN [9]
RP FUNCTION.
RX PubMed=12897257; DOI=10.1105/tpc.012211;
RA Johnson C., Boden E., Arias J.;
RT "Salicylic acid and NPR1 induce the recruitment of trans-activating TGA
RT factors to a defense gene promoter in Arabidopsis.";
RL Plant Cell 15:1846-1858(2003).
RN [10]
RP INTERACTION WITH NPR1 AND NPR4.
RX PubMed=15634206; DOI=10.1111/j.1365-313x.2004.02296.x;
RA Liu G., Holub E.B., Alonso J.M., Ecker J.R., Fobert P.R.;
RT "An Arabidopsis NPR1-like gene, NPR4, is required for disease resistance.";
RL Plant J. 41:304-318(2005).
RN [11]
RP INTERACTION WITH NPR3 AND NPR4.
RX PubMed=17076807; DOI=10.1111/j.1365-313x.2006.02903.x;
RA Zhang Y., Cheng Y.T., Qu N., Zhao Q., Bi D., Li X.;
RT "Negative regulation of defense responses in Arabidopsis by two NPR1
RT paralogs.";
RL Plant J. 48:647-656(2006).
RN [12]
RP INTERACTION WITH GRXC7/ROXY1.
RX PubMed=19218396; DOI=10.1105/tpc.108.064477;
RA Li S., Lauri A., Ziemann M., Busch A., Bhave M., Zachgo S.;
RT "Nuclear activity of ROXY1, a glutaredoxin interacting with TGA factors, is
RT required for petal development in Arabidopsis thaliana.";
RL Plant Cell 21:429-441(2009).
CC -!- FUNCTION: Transcriptional activator that binds specifically to the DNA
CC sequence 5'-TGACG-3'. Recognizes ocs elements like the as-1 motif of
CC the cauliflower mosaic virus 35S promoter. Binding to the as-1-like cis
CC elements mediate auxin- and salicylic acid-inducible transcription.
CC Required to induce the systemic acquired resistance (SAR) via the
CC regulation of pathogenesis-related genes expression. Binding to the as-
CC 1 element of PR-1 promoter is salicylic acid-inducible and mediated by
CC NPR1. Could also bind to the Hex-motif (5'-TGACGTGG-3') another cis-
CC acting element found in plant histone promoters.
CC {ECO:0000269|PubMed:12897257}.
CC -!- SUBUNIT: Binds DNA as a dimer. Interacts with NPR1, NPR3 and NPR4.
CC Interacts with GRXC7/ROXY1. {ECO:0000269|PubMed:10659709,
CC ECO:0000269|PubMed:15634206, ECO:0000269|PubMed:17076807,
CC ECO:0000269|PubMed:19218396}.
CC -!- INTERACTION:
CC Q39234; F4JI72: At4g03250; NbExp=3; IntAct=EBI-541366, EBI-15192535;
CC Q39234; Q9SZ75: At4g12100; NbExp=3; IntAct=EBI-541366, EBI-25519661;
CC Q39234; Q8VYP6: At5g11010; NbExp=3; IntAct=EBI-541366, EBI-21138980;
CC Q39234; Q94K68: At5g27560; NbExp=3; IntAct=EBI-541366, EBI-25521470;
CC Q39234; Q9LT56: ATN1; NbExp=3; IntAct=EBI-541366, EBI-4469161;
CC Q39234; P0DO23: CP2; NbExp=3; IntAct=EBI-541366, EBI-4428219;
CC Q39234; Q96305: GRXC7; NbExp=6; IntAct=EBI-541366, EBI-2257898;
CC Q39234; Q9SGP6: GRXC9; NbExp=3; IntAct=EBI-541366, EBI-1545762;
CC Q39234; Q6NLU2: GRXS7; NbExp=3; IntAct=EBI-541366, EBI-25521272;
CC Q39234; O04341: GRXS9; NbExp=4; IntAct=EBI-541366, EBI-4450582;
CC Q39234; P93002: NPR1; NbExp=9; IntAct=EBI-541366, EBI-1392127;
CC Q39234; Q8L746: NPR3; NbExp=6; IntAct=EBI-541366, EBI-4441365;
CC Q39234; Q9LXU1: PIM1; NbExp=3; IntAct=EBI-541366, EBI-15193025;
CC Q39234; P43273: TGA2; NbExp=3; IntAct=EBI-541366, EBI-541307;
CC Q39234; Q39163: TGA5; NbExp=7; IntAct=EBI-541366, EBI-541381;
CC Q39234; Q39140: TGA6; NbExp=3; IntAct=EBI-541366, EBI-541321;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Expressed in the whole plant.
CC {ECO:0000269|PubMed:8003690, ECO:0000269|PubMed:8628224}.
CC -!- SIMILARITY: Belongs to the bZIP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF86550.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAM64781.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; L10209; AAA32873.1; -; mRNA.
DR EMBL; AC069252; AAF86550.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30193.1; -; Genomic_DNA.
DR EMBL; AY087225; AAM64781.1; ALT_INIT; mRNA.
DR PIR; F86353; F86353.
DR PIR; S46523; S46523.
DR RefSeq; NP_564156.1; NM_102057.4.
DR PDB; 7TAC; EM; 3.60 A; C/D/E/F=87-384.
DR PDB; 7TAD; EM; 3.60 A; C/D=87-384.
DR PDB; 7TAE; X-ray; 1.50 A; A=161-384.
DR PDBsum; 7TAC; -.
DR PDBsum; 7TAD; -.
DR PDBsum; 7TAE; -.
DR AlphaFoldDB; Q39234; -.
DR SMR; Q39234; -.
DR BioGRID; 24051; 33.
DR IntAct; Q39234; 29.
DR STRING; 3702.AT1G22070.1; -.
DR PaxDb; Q39234; -.
DR PRIDE; Q39234; -.
DR ProteomicsDB; 246472; -.
DR EnsemblPlants; AT1G22070.1; AT1G22070.1; AT1G22070.
DR GeneID; 838812; -.
DR Gramene; AT1G22070.1; AT1G22070.1; AT1G22070.
DR KEGG; ath:AT1G22070; -.
DR Araport; AT1G22070; -.
DR TAIR; locus:2030551; AT1G22070.
DR eggNOG; ENOG502QS1H; Eukaryota.
DR HOGENOM; CLU_024782_1_0_1; -.
DR InParanoid; Q39234; -.
DR OMA; DICLNHY; -.
DR OrthoDB; 752703at2759; -.
DR PhylomeDB; Q39234; -.
DR PRO; PR:Q39234; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q39234; baseline and differential.
DR Genevisible; Q39234; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0005516; F:calmodulin binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0042742; P:defense response to bacterium; IMP:TAIR.
DR GO; GO:0009626; P:plant-type hypersensitive response; IEA:UniProtKB-KW.
DR GO; GO:0009862; P:systemic acquired resistance, salicylic acid mediated signaling pathway; IPI:TAIR.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR InterPro; IPR004827; bZIP.
DR InterPro; IPR046347; bZIP_sf.
DR InterPro; IPR025422; TGA_domain.
DR Pfam; PF07716; bZIP_2; 1.
DR Pfam; PF14144; DOG1; 1.
DR SMART; SM00338; BRLZ; 1.
DR SUPFAM; SSF57959; SSF57959; 1.
DR PROSITE; PS50217; BZIP; 1.
DR PROSITE; PS00036; BZIP_BASIC; 1.
DR PROSITE; PS51806; DOG1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Coiled coil; DNA-binding; Hypersensitive response;
KW Nucleus; Plant defense; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..384
FT /note="Transcription factor TGA3"
FT /id="PRO_0000076555"
FT DOMAIN 96..159
FT /note="bZIP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT DOMAIN 167..379
FT /note="DOG1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01147"
FT REGION 36..70
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 76..95
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 98..118
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 124..138
FT /note="Leucine-zipper"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT COILED 98..151
FT /evidence="ECO:0000255"
FT COILED 267..296
FT /evidence="ECO:0000255"
FT COMPBIAS 36..58
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 58
FT /note="V -> F (in Ref. 4; AAM64781)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 384 AA; 43755 MW; 48F965F62A2980A9 CRC64;
MEMMSSSSST TQVVSFRDMG MYEPFQQLSG WESPFKSDIN NITSNQNNNQ SSSTTLEVDA
RPEADDNNRV NYTSVYNNSL EAEPSSNNDQ DEDRINDKMK RRLAQNREAA RKSRLRKKAH
VQQLEESRLK LSQLEQELVR ARQQGLCVRN SSDTSYLGPA GNMNSGIAAF EMEYTHWLEE
QNRRVSEIRT ALQAHIGDIE LKMLVDSCLN HYANLFRMKA DAAKADVFFL MSGMWRTSTE
RFFQWIGGFR PSELLNVVMP YVEPLTDQQL LEVRNLQQSS QQAEEALSQG LDKLQQGLVE
SIAIQIKVVE SVNHGAPMAS AMENLQALES FVNQADHLRQ QTLQQMSKIL TTRQAARGLL
ALGEYFHRLR ALSSLWAARP REHT
