TGA6_ARATH
ID TGA6_ARATH Reviewed; 330 AA.
AC Q39140; Q9C7D1; Q9LHI1;
DT 01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2004, sequence version 2.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Transcription factor TGA6;
DE AltName: Full=bZIP transcription factor 45;
DE Short=AtbZIP45;
GN Name=TGA6; Synonyms=BZIP45; OrderedLocusNames=At3g12250;
GN ORFNames=F28J15.6, MQC3.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia; TISSUE=Leaf;
RA Xiang C., Miao Z.-H., Lam E.;
RT "Isolation of TGA6, a new member of the TGA family of bZIP transcription
RT factors in Arabidopsis thaliana.";
RL (er) Plant Gene Register PGR95-063(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=11906833; DOI=10.1016/s1360-1385(01)02223-3;
RA Jakoby M., Weisshaar B., Droege-Laser W., Vicente-Carbajosa J.,
RA Tiedemann J., Kroj T., Parcy F.;
RT "bZIP transcription factors in Arabidopsis.";
RL Trends Plant Sci. 7:106-111(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [6]
RP CHARACTERIZATION, AND DNA-BINDING.
RX PubMed=9225852; DOI=10.1023/a:1005873500238;
RA Xiang C., Miao Z.-H., Lam E.;
RT "DNA-binding properties, genomic organization and expression pattern of
RT TGA6, a new member of the TGA family of bZIP transcription factors in
RT Arabidopsis thaliana.";
RL Plant Mol. Biol. 34:403-415(1997).
RN [7]
RP INTERACTION WITH NPR1.
RX PubMed=10659709; DOI=10.1094/mpmi.2000.13.2.191;
RA Zhou J.-M., Trifa Y., Silva H., Pontier D., Lam E., Shah J., Klessig D.F.;
RT "NPR1 differentially interacts with members of the TGA/OBF family of
RT transcription factors that bind an element of the PR-1 gene required for
RT induction by salicylic acid.";
RL Mol. Plant Microbe Interact. 13:191-202(2000).
RN [8]
RP INTERACTION WITH NPR1 AND NPR4.
RX PubMed=15634206; DOI=10.1111/j.1365-313x.2004.02296.x;
RA Liu G., Holub E.B., Alonso J.M., Ecker J.R., Fobert P.R.;
RT "An Arabidopsis NPR1-like gene, NPR4, is required for disease resistance.";
RL Plant J. 41:304-318(2005).
RN [9]
RP INTERACTION WITH NPR3 AND NPR4.
RX PubMed=17076807; DOI=10.1111/j.1365-313x.2006.02903.x;
RA Zhang Y., Cheng Y.T., Qu N., Zhao Q., Bi D., Li X.;
RT "Negative regulation of defense responses in Arabidopsis by two NPR1
RT paralogs.";
RL Plant J. 48:647-656(2006).
RN [10]
RP INTERACTION WITH GRXC9/GRX480.
RX PubMed=17397508; DOI=10.1111/j.1365-313x.2007.03039.x;
RA Ndamukong I., Abdallat A.A., Thurow C., Fode B., Zander M., Weigel R.,
RA Gatz C.;
RT "SA-inducible Arabidopsis glutaredoxin interacts with TGA factors and
RT suppresses JA-responsive PDF1.2 transcription.";
RL Plant J. 50:128-139(2007).
CC -!- FUNCTION: Transcriptional activator that binds specifically to the DNA
CC sequence 5'-TGACG-3'. Recognizes ocs elements like the as-1 motif of
CC the cauliflower mosaic virus 35S promoter. Binding to the as-1-like cis
CC elements mediate auxin- and salicylic acid-inducible transcription. May
CC be involved in the induction of the systemic acquired resistance (SAR)
CC via its interaction with NPR1. Could also bind to the Hex-motif (5'-
CC TGACGTGG-3') another cis-acting element found in plant histone
CC promoters (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Binds DNA as a dimer. Interacts with NPR1, NPR3 and NPR4.
CC Interacts with GRXC9/GRX480. {ECO:0000269|PubMed:10659709,
CC ECO:0000269|PubMed:15634206, ECO:0000269|PubMed:17076807,
CC ECO:0000269|PubMed:17397508}.
CC -!- INTERACTION:
CC Q39140; Q9ZUM0: At2g02160; NbExp=3; IntAct=EBI-541321, EBI-15206592;
CC Q39140; Q9SGP6: GRXC9; NbExp=4; IntAct=EBI-541321, EBI-1545762;
CC Q39140; P93002: NPR1; NbExp=5; IntAct=EBI-541321, EBI-1392127;
CC Q39140; Q9XE58: SCL14; NbExp=3; IntAct=EBI-541321, EBI-25523217;
CC Q39140; Q39234: TGA3; NbExp=3; IntAct=EBI-541321, EBI-541366;
CC Q39140; Q39163: TGA5; NbExp=3; IntAct=EBI-541321, EBI-541381;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q39140-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q39140-2; Sequence=VSP_009468;
CC -!- TISSUE SPECIFICITY: Expressed predominantly in roots and flowers.
CC -!- DEVELOPMENTAL STAGE: Expressed primarily in roots of young seedlings
CC and later expressed in aging cotyledons, mesophyll cells of hydathodes
CC on leaf margins, vascular tissue and trichomes of senescing rosette
CC leaves. Also detected in young lateral roots and in mature pollen
CC grains.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to an intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the bZIP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB03134.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; L42327; AAC37470.1; -; mRNA.
DR EMBL; AJ320540; CAC42807.1; -; mRNA.
DR EMBL; AC069472; AAG51079.1; -; Genomic_DNA.
DR EMBL; AP002047; BAB03134.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE75172.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75173.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75174.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM63961.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM63962.1; -; Genomic_DNA.
DR RefSeq; NP_001326019.1; NM_001337984.1. [Q39140-1]
DR RefSeq; NP_001326020.1; NM_001337985.1. [Q39140-1]
DR RefSeq; NP_566415.3; NM_112061.6. [Q39140-1]
DR RefSeq; NP_974292.1; NM_202563.3. [Q39140-1]
DR RefSeq; NP_974293.1; NM_202564.2. [Q39140-2]
DR AlphaFoldDB; Q39140; -.
DR BioGRID; 5739; 16.
DR IntAct; Q39140; 15.
DR STRING; 3702.AT3G12250.4; -.
DR iPTMnet; Q39140; -.
DR PRIDE; Q39140; -.
DR ProteomicsDB; 234213; -. [Q39140-1]
DR EnsemblPlants; AT3G12250.1; AT3G12250.1; AT3G12250. [Q39140-1]
DR EnsemblPlants; AT3G12250.2; AT3G12250.2; AT3G12250. [Q39140-1]
DR EnsemblPlants; AT3G12250.3; AT3G12250.3; AT3G12250. [Q39140-2]
DR EnsemblPlants; AT3G12250.6; AT3G12250.6; AT3G12250. [Q39140-1]
DR EnsemblPlants; AT3G12250.7; AT3G12250.7; AT3G12250. [Q39140-1]
DR GeneID; 820405; -.
DR Gramene; AT3G12250.1; AT3G12250.1; AT3G12250. [Q39140-1]
DR Gramene; AT3G12250.2; AT3G12250.2; AT3G12250. [Q39140-1]
DR Gramene; AT3G12250.3; AT3G12250.3; AT3G12250. [Q39140-2]
DR Gramene; AT3G12250.6; AT3G12250.6; AT3G12250. [Q39140-1]
DR Gramene; AT3G12250.7; AT3G12250.7; AT3G12250. [Q39140-1]
DR KEGG; ath:AT3G12250; -.
DR Araport; AT3G12250; -.
DR eggNOG; ENOG502QU32; Eukaryota.
DR InParanoid; Q39140; -.
DR PhylomeDB; Q39140; -.
DR PRO; PR:Q39140; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q39140; baseline and differential.
DR Genevisible; Q39140; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IEA:UniProt.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR InterPro; IPR004827; bZIP.
DR InterPro; IPR046347; bZIP_sf.
DR InterPro; IPR018524; DNA/RNA_endonuclease_AS.
DR InterPro; IPR025422; TGA_domain.
DR Pfam; PF00170; bZIP_1; 1.
DR Pfam; PF14144; DOG1; 1.
DR SMART; SM00338; BRLZ; 1.
DR SUPFAM; SSF57959; SSF57959; 1.
DR PROSITE; PS50217; BZIP; 1.
DR PROSITE; PS00036; BZIP_BASIC; 1.
DR PROSITE; PS51806; DOG1; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Coiled coil; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..330
FT /note="Transcription factor TGA6"
FT /id="PRO_0000076558"
FT DOMAIN 44..107
FT /note="bZIP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT DOMAIN 111..327
FT /note="DOG1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01147"
FT REGION 1..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 46..66
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 72..86
FT /note="Leucine-zipper"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT COILED 45..142
FT /evidence="ECO:0000255"
FT COILED 217..233
FT /evidence="ECO:0000255"
FT COMPBIAS 10..45
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..22
FT /note="MADTSSRTDVSTDGDTDHRDLG -> MHSLNETVIPDVDYMQ (in
FT isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_009468"
FT CONFLICT 74
FT /note="N -> D (in Ref. 1; AAC37470)"
FT /evidence="ECO:0000305"
FT CONFLICT 82
FT /note="L -> V (in Ref. 1; AAC37470)"
FT /evidence="ECO:0000305"
FT CONFLICT 110
FT /note="G -> GG (in Ref. 1; AAC37470/BAB03134)"
FT /evidence="ECO:0000305"
FT CONFLICT 167
FT /note="A -> S (in Ref. 1; AAC37470)"
FT /evidence="ECO:0000305"
FT CONFLICT 194
FT /note="R -> P (in Ref. 1; AAC37470)"
FT /evidence="ECO:0000305"
FT CONFLICT 272
FT /note="Q -> K (in Ref. 1; AAC37470)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 330 AA; 36850 MW; 2A55F2F336648A48 CRC64;
MADTSSRTDV STDGDTDHRD LGSDRGHMHA AASDSSDRSK DKLDQKTLRR LAQNREAARK
SRLRKKAYVQ QLENSRLKLT QLEQELQRAR QQGVFISSSG DQAHSTGGNG ALAFDAEHSR
WLEEKNRQMN ELRSALNAHA GDTELRIIVD GVMAHYEELF RIKSNAAKND VFHLLSGMWK
TPAERCFLWL GGFRSSELLK LLANQLEPMT ERQVMGINSL QQTSQQAEDA LSQGMESLQQ
SLADTLSSGT LGSSSSDNVA SYMGQMAMAM GQLGTLEGFI RQADNLRLQT LQQMLRVLTT
RQSARALLAI HDYSSRLRAL SSLWLARPRE
