ID   TGB1_ASPVP              Reviewed;         223 AA.
AC   Q64963;
DT   02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   23-FEB-2022, entry version 73.
DE   RecName: Full=Movement and silencing protein TGBp1;
DE   AltName: Full=25 kDa protein;
DE   AltName: Full=Silencing suppressor P25;
DE   AltName: Full=Triple gene block 1 protein;
DE            Short=TGBp1;
GN   ORFNames=ORF2;
OS   Apple stem pitting virus (isolate PA66) (ASPV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Tymovirales; Betaflexiviridae; Quinvirinae; Foveavirus.
OX   NCBI_TaxID=651356;
OH   NCBI_TaxID=23159; Crataegus (hawthorn).
OH   NCBI_TaxID=106566; Malus sieboldii.
OH   NCBI_TaxID=3752; Malus sylvestris.
OH   NCBI_TaxID=23211; Pyrus communis (Pear) (Pyrus domestica).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=8021584; DOI=10.1099/0022-1317-75-7-1535;
RA   Jelkmann W.;
RT   "Nucleotide sequences of apple stem pitting virus and of the coat protein
RT   gene of a similar virus from pear associated with vein yellows disease and
RT   their relationship with potex- and carlaviruses.";
RL   J. Gen. Virol. 75:1535-1542(1994).
CC   -!- FUNCTION: Transports viral genome to neighboring plant cells directly
CC       through plasmosdesmata, without any budding. The movement protein
CC       allows efficient cell to cell propagation, by bypassing the host cell
CC       wall barrier. Increases plasmodesma size exclusion limit. Acts as a
CC       suppressor of RNA-mediated gene silencing, also known as post-
CC       transcriptional gene silencing (PTGS), a mechanism of plant viral
CC       defense that limits the accumulation of viral RNAs (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer and homooligomer. Interacts with capsid protein.
CC       Interacts with host AGO1; this interaction targets the host protein for
CC       degradation, thereby suppressing the antiviral RNA silencing (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250}.
CC   -!- MISCELLANEOUS: TGBp1, TGBp2 and TGBp3 seem to act together for cell-to-
CC       cell propagation. TGBp1 is the main movement protein that physically
CC       cross the plasmodesma with the viral genome. TGBp2 and TGBp3 would
CC       facilitate TGBp1 function.
CC   -!- SIMILARITY: Belongs to the Tymovirales TGBp1 protein family.
CC       {ECO:0000305}.
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DR   EMBL; D21829; BAA04854.1; -; Genomic_RNA.
DR   RefSeq; NP_604465.1; NC_003462.2.
DR   GeneID; 935273; -.
DR   KEGG; vg:935273; -.
DR   Proteomes; UP000000678; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR   InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01443; Viral_helicase1; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51657; PSRV_HELICASE; 1.
PE   3: Inferred from homology;
KW   Host cytoplasm; Host-virus interaction; Reference proteome; RNA-binding;
KW   Suppressor of RNA silencing; Transport; Viral movement protein.
FT   CHAIN           1..223
FT                   /note="Movement and silencing protein TGBp1"
FT                   /id="PRO_0000401090"
FT   DOMAIN          1..136
FT                   /note="(+)RNA virus helicase ATP-binding"
FT   DOMAIN          137..223
FT                   /note="(+)RNA virus helicase C-terminal"
SQ   SEQUENCE   223 AA;  25210 MW;  8D6AADE1B6C9380A CRC64;
     METVLSLLNE FGFERTVEPL SDPIVVHAVP GSGKTTLIKQ ALIRNNNIEA VTFGVPEKAN
     IHGTYIKKAR QGQRGRGNYS ILDEYLSGEY STGFNCLFSD PYQNHGDCLR AHFIGRCSHR
     FGRQTVQILR DLGYNIASSK EDIVEKKNIF QLIEPEGVII CLEKGVEDFL KWHSVEYKFP
     CQVRGATFDI VTFIHEKPLE ELVGPDLFVA LTRHRSKLVL VSN