ID   TGB1_CVB                Reviewed;         231 AA.
AC   P37988;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   23-FEB-2022, entry version 66.
DE   RecName: Full=Movement and silencing protein TGBp1;
DE   AltName: Full=25 kDa protein;
DE   AltName: Full=Silencing suppressor P25;
DE   AltName: Full=Triple gene block 1 protein;
DE            Short=TGBp1;
GN   ORFNames=ORF2;
OS   Chrysanthemum virus B (CVB).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus.
OX   NCBI_TaxID=12165;
OH   NCBI_TaxID=41568; Chrysanthemum morifolium (Florist's daisy) (Dendranthema grandiflorum).
OH   NCBI_TaxID=109564; Gynura.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=1919520; DOI=10.1099/0022-1317-72-10-2333;
RA   Levay K., Zavriev S.;
RT   "Nucleotide sequence and gene organization of the 3'-terminal region of
RT   chrysanthemum virus B genomic RNA.";
RL   J. Gen. Virol. 72:2333-2337(1991).
CC   -!- FUNCTION: Transports viral genome to neighboring plant cells directly
CC       through plasmosdesmata, without any budding. The movement protein
CC       allows efficient cell to cell propagation, by bypassing the host cell
CC       wall barrier. Increases plasmodesma size exclusion limit. Acts as a
CC       suppressor of RNA-mediated gene silencing, also known as post-
CC       transcriptional gene silencing (PTGS), a mechanism of plant viral
CC       defense that limits the accumulation of viral RNAs (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer and homooligomer. Interacts with capsid protein.
CC       Interacts with host AGO1; this interaction targets the host protein for
CC       degradation, thereby suppressing the antiviral RNA silencing (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250}.
CC   -!- MISCELLANEOUS: TGBp1, TGBp2 and TGBp3 seem to act together for cell-to-
CC       cell propagation. TGBp1 is the main movement protein that physically
CC       cross the plasmodesma with the viral genome. TGBp2 and TGBp3 would
CC       facilitate TGBp1 function.
CC   -!- SIMILARITY: Belongs to the Tymovirales TGBp1 protein family.
CC       {ECO:0000305}.
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DR   EMBL; S60150; AAB20077.1; -; Genomic_RNA.
DR   PIR; JQ1247; JQ1247.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR   InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR   Pfam; PF01443; Viral_helicase1; 1.
DR   PROSITE; PS51657; PSRV_HELICASE; 1.
PE   3: Inferred from homology;
KW   Host cytoplasm; Host-virus interaction; RNA-binding;
KW   Suppressor of RNA silencing; Transport; Viral movement protein.
FT   CHAIN           1..231
FT                   /note="Movement and silencing protein TGBp1"
FT                   /id="PRO_0000222577"
FT   DOMAIN          1..123
FT                   /note="(+)RNA virus helicase ATP-binding"
FT   DOMAIN          124..231
FT                   /note="(+)RNA virus helicase C-terminal"
SQ   SEQUENCE   231 AA;  25824 MW;  2D6C3FA30580E0AA CRC64;
     MDVFVKILSD FGFTRLSSSL HVPIVVHCMP GAGKSSCIRA LLKADSRFVA YTLGVPDPCN
     LDGLCIKAFT GSVDLKYFNI LDEYARYNGD ASDFFALFGD PVQSPPRNLY RAHFKAVLSK
     RFGSCTAQLL RELGFEVEST KEDLVSIRGL YDFDPVGTVI YYEKEIGCLL RAHSIEAYEP
     EEVVGKTFET VTFVTAENHI PAESRHLVYQ CLTRHRSVLH LMTPDASYTS T