ID   TGB1_P1AMV              Reviewed;         230 AA.
AC   Q07517;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   02-DEC-2020, entry version 77.
DE   RecName: Full=Movement and silencing protein TGBp1;
DE   AltName: Full=25 kDa protein;
DE   AltName: Full=Silencing suppressor P25;
DE   AltName: Full=Triple gene block 1 protein;
DE            Short=TGBp1;
GN   ORFNames=ORF2;
OS   Plantago asiatica mosaic potexvirus (P1AMV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Tymovirales; Alphaflexiviridae; Potexvirus.
OX   NCBI_TaxID=28354;
OH   NCBI_TaxID=197796; Plantago asiatica.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=8113746; DOI=10.1099/0022-1317-75-2-259;
RA   Solovyev A.G., Novikov V.K., Merits A., Savenkov E.I., Zelenina D.A.,
RA   Tyulkina L.G., Morozov S.Y.;
RT   "Genome characterization and taxonomy of Plantago asiatica mosaic
RT   potexvirus.";
RL   J. Gen. Virol. 75:259-267(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=8477237;
RA   Solovyev A.G., Novikov V.K., Morozov S.I., Kagramanov V.N., Atabekov I.G.;
RT   "Primary structure of the triple block RNA genes of the Plantago asiatica
RT   mosaic virus.";
RL   Dokl. Akad. Nauk 328:625-628(1993).
RN   [3]
RP   REVIEW.
RX   PubMed=15828680; DOI=10.1094/mpmi-18-0283;
RA   Verchot-Lubicz J.;
RT   "A new cell-to-cell transport model for Potexviruses.";
RL   Mol. Plant Microbe Interact. 18:283-290(2005).
CC   -!- FUNCTION: Transports viral genome to neighboring plant cells directly
CC       through plasmosdesmata, without any budding. The movement protein
CC       allows efficient cell to cell propagation, by bypassing the host cell
CC       wall barrier. Increases plasmodesma size exclusion limit. Acts as a
CC       suppressor of RNA-mediated gene silencing, also known as post-
CC       transcriptional gene silencing (PTGS), a mechanism of plant viral
CC       defense that limits the accumulation of viral RNAs (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer and homooligomer. Interacts with capsid protein.
CC       Interacts with host AGO1; this interaction targets the host protein for
CC       degradation, thereby suppressing the antiviral RNA silencing (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250}.
CC   -!- MISCELLANEOUS: TGBp1, TGBp2 and TGBp3 seem to act together for cell-to-
CC       cell propagation. TGBp1 is the main movement protein that physically
CC       cross the plasmodesma with the viral genome. TGBp2 and TGBp3 would
CC       facilitate TGBp1 function.
CC   -!- SIMILARITY: Belongs to the Tymovirales TGBp1 protein family.
CC       {ECO:0000305}.
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DR   EMBL; Z21647; CAA79762.1; -; Genomic_RNA.
DR   EMBL; S61526; AAB26348.1; -; Genomic_RNA.
DR   PIR; S34231; S34231.
DR   RefSeq; NP_620837.1; NC_003849.1.
DR   SMR; Q07517; -.
DR   GeneID; 944433; -.
DR   KEGG; vg:944433; -.
DR   Proteomes; UP000009190; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR   InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR   Pfam; PF01443; Viral_helicase1; 1.
DR   PROSITE; PS51657; PSRV_HELICASE; 1.
PE   3: Inferred from homology;
KW   Host cytoplasm; Host-virus interaction; Reference proteome; RNA-binding;
KW   Suppressor of RNA silencing; Transport; Viral movement protein.
FT   CHAIN           1..230
FT                   /note="Movement and silencing protein TGBp1"
FT                   /id="PRO_0000222568"
FT   DOMAIN          1..114
FT                   /note="(+)RNA virus helicase ATP-binding"
FT   DOMAIN          115..230
FT                   /note="(+)RNA virus helicase C-terminal"
FT   CONFLICT        27
FT                   /note="H -> L (in Ref. 2; AAB26348)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   230 AA;  24687 MW;  F8D2BC14EC9A812C CRC64;
     MDSIINALTS NNFQRTNTPI SKPLVVHAVA GAGKTTLIQN LLPEHPNLAA QTAGSPQTPN
     LTGAFIRKLT CPESNKINLL DEYAALQPLK GSWDVVLADP LQHPGLALRP HFIKSVSHRL
     CPATTRLISK LVCPCTSSRT EESTIQFSGL FEGPLLGTVI ALDQTTQALL TAHGAHFLCP
     TAALGLEFDT VTVVSALPLE EVADKVGLYI SLSRHRSQLH VRSPPPHPSH