TGB1_PCV87
ID TGB1_PCV87 Reviewed; 446 AA.
AC Q08317;
DT 31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Movement protein TGB1;
DE EC=3.6.4.- {ECO:0000250|UniProtKB:P04867};
DE AltName: Full=P51;
DE AltName: Full=Triple gene block 1 protein;
DE Short=TGBp1;
OS Peanut clump virus (isolate 87/TGTA2) (PCV).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Martellivirales; Virgaviridae; Pecluvirus.
OX NCBI_TaxID=652837;
OH NCBI_TaxID=3818; Arachis hypogaea (Peanut).
OH NCBI_TaxID=4555; Setaria italica (Foxtail millet) (Panicum italicum).
OH NCBI_TaxID=91525; Sorghum arundinaceum.
OH NCBI_TaxID=4558; Sorghum bicolor (Sorghum) (Sorghum vulgare).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=8317107; DOI=10.1006/viro.1993.1343;
RA Manohar S.K., Guilley H., Dollet M., Richards K., Jonard G.;
RT "Nucleotide sequence and genetic organization of peanut clump virus RNA 2
RT and partial characterization of deleted forms.";
RL Virology 195:33-41(1993).
RN [2]
RP FUNCTION.
RX PubMed=9721240; DOI=10.1006/viro.1998.9287;
RA Herzog E., Hemmer O., Hauser S., Meyer G., Bouzoubaa S., Fritsch C.;
RT "Identification of genes involved in replication and movement of peanut
RT clump virus.";
RL Virology 248:312-322(1998).
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=10544148; DOI=10.1006/viro.1999.9997;
RA Erhardt M., Stussi-Garaud C., Guilley H., Richards K.E., Jonard G.,
RA Bouzoubaa S.;
RT "The first triple gene block protein of peanut clump virus localizes to the
RT plasmodesmata during virus infection.";
RL Virology 264:220-229(1999).
CC -!- FUNCTION: Participates in the transport of viral genome to neighboring
CC plant cells directly through plasmodesmata, without any budding
CC (PubMed:9721240). Multifunctional movement protein with RNA-binding,
CC ATPase and helicase activities (By similarity). Engages in homologous
CC interactions leading to the formation of a ribonucleoprotein complex
CC containing plus-sense viral RNAs (vRNPs) (By similarity). ATPase
CC activity is probably required for vRNPs movement complex assembly (By
CC similarity). Intracellular delivery of TGBp1-containing vRNPs to
CC plasmodesmata is facilitated by TGBp2 and TGBp3 (By similarity).
CC {ECO:0000250|UniProtKB:P04867, ECO:0000269|PubMed:9721240}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216;
CC Evidence={ECO:0000250|UniProtKB:P04867};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:P04867};
CC -!- SUBUNIT: Homooligomer. Interacts with movement protein TGB3. TGB1-TGB3-
CC TGB2 complex formation is enhanced by ATP hydrolysis. Interacts with
CC the suppressor of RNA silencing (via N-terminus).
CC {ECO:0000250|UniProtKB:P04867}.
CC -!- SUBCELLULAR LOCATION: Host cell junction, host plasmodesma
CC {ECO:0000269|PubMed:10544148}. Host nucleus
CC {ECO:0000250|UniProtKB:P04867}. Host cytoplasm
CC {ECO:0000250|UniProtKB:P04867}. Host nucleus, host nucleolus
CC {ECO:0000250|UniProtKB:P04867}. Host cytoplasm, host cytoskeleton
CC {ECO:0000250|UniProtKB:Q9IV54}. Note=TGB1 nuclear-cytoplasmic
CC trafficking is required for cell-to-cell movement and systemic
CC infection (By similarity). Associates with host microtubules (By
CC similarity). {ECO:0000250|UniProtKB:P04867,
CC ECO:0000250|UniProtKB:Q9IV54}.
CC -!- SIMILARITY: Belongs to the virgaviridae/benyvirus TGB1 movement protein
CC family. {ECO:0000305}.
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DR EMBL; L07269; AAA17438.1; -; Unassigned_DNA.
DR RefSeq; NP_620030.1; NC_003668.1.
DR SMR; Q08317; -.
DR GeneID; 991040; -.
DR KEGG; vg:991040; -.
DR Proteomes; UP000001668; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0044219; C:host cell plasmodesma; IEA:UniProtKB-SubCell.
DR GO; GO:0044163; C:host cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01443; Viral_helicase1; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51657; PSRV_HELICASE; 1.
PE 3: Inferred from homology;
KW ATP-binding; Host cell junction; Host cytoplasm; Host cytoskeleton;
KW Host nucleus; Hydrolase; Nucleotide-binding; Reference proteome; Transport;
KW Viral movement protein.
FT CHAIN 1..446
FT /note="Movement protein TGB1"
FT /id="PRO_0000409149"
FT DOMAIN 160..303
FT /note="(+)RNA virus helicase ATP-binding"
FT DOMAIN 304..444
FT /note="(+)RNA virus helicase C-terminal"
FT REGION 40..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 193..200
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 446 AA; 51056 MW; FD783154FF103830 CRC64;
MEWRRAFSKK KLEEKVKNMD AKQATDYLLE KVDEQRNLEN KLDKRLQNTR KKNKNKEKTR
TWAEKYPPVD YYSPEFVENF MKDMRREEFE KSEERRGHKQ VRLGSDNFVG DDPLKVLSEE
ALKAGFQHTG KVMKRFPADV FEKSKFIGMY DRHLTTLREK ACCKKERNQI QSKLIQLRQL
KPSCDFLAGT VSGVPGSGKS TLLKNVQKKL KNSVCLLANK ELKGDFAGVP SVFSVEEMLL
SAVPSSFNVM LVDEYTLTQS AEILLLQRKL GAKIVVLFGD REQGNTNKLT SPEWLHVPIV
FSSDSSHRFG PETAKFCEDQ GFSLEGRGGE DKIVKGDYEG EGEDTEVNLC FTEETKADLA
EVQVEAFLVS SVQGRTFSSV SLFVRENDKP VFSDPHLRLV AITRHRKLLS IRADPEVWVS
FMFATREGEE VDTHCYGEEH RPDEAE
