ID   TGB1_POPMV              Reviewed;         232 AA.
AC   Q02109; Q629I9;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 2.
DT   02-DEC-2020, entry version 74.
DE   RecName: Full=Movement and silencing protein TGBp1;
DE   AltName: Full=25 kDa protein;
DE   AltName: Full=Silencing suppressor P25;
DE   AltName: Full=Triple gene block 1 protein;
DE            Short=TGBp1;
GN   ORFNames=ORF2;
OS   Poplar mosaic virus (isolate ATCC Pv275) (PMV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus.
OX   NCBI_TaxID=31709;
OH   NCBI_TaxID=73824; Populus balsamifera (Balsam poplar).
OH   NCBI_TaxID=3696; Populus deltoides (Eastern poplar) (Eastern cottonwood).
OH   NCBI_TaxID=75703; Populus maximowiczii (Japanese poplar).
OH   NCBI_TaxID=3691; Populus nigra (Lombardy poplar).
OH   NCBI_TaxID=3694; Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=1629709; DOI=10.1099/0022-1317-73-7-1887;
RA   Henderson J., Gibbs M.J., Edwards M.-L., Clarke V.A., Gardner K.A.,
RA   Cooper J.I.;
RT   "Partial nucleotide sequence of poplar mosaic virus RNA confirms its
RT   classification as a carlavirus.";
RL   J. Gen. Virol. 73:1887-1890(1992).
RN   [2]
RP   SEQUENCE REVISION.
RX   PubMed=15664047; DOI=10.1016/j.jviromet.2004.10.007;
RA   Naylor M., Reeves J., Cooper J.I., Edwards M.-L., Wang H.;
RT   "Construction and properties of a gene-silencing vector based on Poplar
RT   mosaic virus (genus Carlavirus).";
RL   J. Virol. Methods 124:27-36(2005).
CC   -!- FUNCTION: Transports viral genome to neighboring plant cells directly
CC       through plasmosdesmata, without any budding. The movement protein
CC       allows efficient cell to cell propagation, by bypassing the host cell
CC       wall barrier. Increases plasmodesma size exclusion limit. Acts as a
CC       suppressor of RNA-mediated gene silencing, also known as post-
CC       transcriptional gene silencing (PTGS), a mechanism of plant viral
CC       defense that limits the accumulation of viral RNAs (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer and homooligomer. Interacts with capsid protein.
CC       Interacts with host AGO1; this interaction targets the host protein for
CC       degradation, thereby suppressing the antiviral RNA silencing (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250}.
CC   -!- MISCELLANEOUS: TGBp1, TGBp2 and TGBp3 seem to act together for cell-to-
CC       cell propagation. TGBp1 is the main movement protein that physically
CC       cross the plasmodesma with the viral genome. TGBp2 and TGBp3 would
CC       facilitate TGBp1 function.
CC   -!- SIMILARITY: Belongs to the Tymovirales TGBp1 protein family.
CC       {ECO:0000305}.
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DR   EMBL; X65102; CAH55774.1; -; Genomic_RNA.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR   InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR   Pfam; PF01443; Viral_helicase1; 1.
DR   PROSITE; PS51657; PSRV_HELICASE; 1.
PE   3: Inferred from homology;
KW   Host cytoplasm; Host-virus interaction; RNA-binding;
KW   Suppressor of RNA silencing; Transport; Viral movement protein.
FT   CHAIN           1..232
FT                   /note="Movement and silencing protein TGBp1"
FT                   /id="PRO_0000222580"
FT   DOMAIN          1..117
FT                   /note="(+)RNA virus helicase ATP-binding"
FT   DOMAIN          118..232
FT                   /note="(+)RNA virus helicase C-terminal"
SQ   SEQUENCE   232 AA;  25754 MW;  0B804B5328559F4E CRC64;
     MDVLINKLAS CNFSRTRNQV GKPCIINCVP GAGKSTLIRE LLNSDSRFRA YTFGEADPKN
     LSGRRILPAS ELQNAPQGAL IIIDEYTEGS WEPGKICAAF GDPIQSRGPG IVADFVCNKT
     KRFGSSTCEL LNGFGFEIHS EKEDICLVRD FFEVEPEGTV IAFESEVKDI LARHFVEFED
     ICSIRGKTFE EVTFFTASNS IPEHLRADFF QCLTRHKNKL TIACPDATFA PS