ID   TGB1_PVMR               Reviewed;         229 AA.
AC   P17526;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   02-DEC-2020, entry version 84.
DE   RecName: Full=Movement and silencing protein TGBp1;
DE   AltName: Full=25 kDa protein;
DE   AltName: Full=Silencing suppressor P25;
DE   AltName: Full=Triple gene block 1 protein;
DE            Short=TGBp1;
GN   ORFNames=ORF2;
OS   Potato virus M (strain Russian) (PVM).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus.
OX   NCBI_TaxID=12168;
OH   NCBI_TaxID=4113; Solanum tuberosum (Potato).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2738581; DOI=10.1099/0022-1317-70-7-1861;
RA   Rupasov V.V., Morozov S.Y., Kanyuka K.V., Zavriev S.K.;
RT   "Partial nucleotide sequence of potato virus M RNA shows similarities to
RT   protexviruses in gene arrangement and the encoded amino acid sequences.";
RL   J. Gen. Virol. 70:1861-1869(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=1990070; DOI=10.1099/0022-1317-72-1-9;
RA   Zavriev S.K., Kanyuka K.V., Levay K.E.;
RT   "The genome organization of potato virus M RNA.";
RL   J. Gen. Virol. 72:9-14(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=1944258;
RA   Zavriev S.K., Kaniuka K.V., Levai K.E.;
RT   "Complete nucleotide sequence of genomic RNA of the potato M-virus.";
RL   Mol. Biol. (Mosk.) 25:761-769(1991).
CC   -!- FUNCTION: Transports viral genome to neighboring plant cells directly
CC       through plasmosdesmata, without any budding. The movement protein
CC       allows efficient cell to cell propagation, by bypassing the host cell
CC       wall barrier. Increases plasmodesma size exclusion limit. Acts as a
CC       suppressor of RNA-mediated gene silencing, also known as post-
CC       transcriptional gene silencing (PTGS), a mechanism of plant viral
CC       defense that limits the accumulation of viral RNAs (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer and homooligomer. Interacts with capsid protein.
CC       Interacts with host AGO1; this interaction targets the host protein for
CC       degradation, thereby suppressing the antiviral RNA silencing (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250}.
CC   -!- MISCELLANEOUS: TGBp1, TGBp2 and TGBp3 seem to act together for cell-to-
CC       cell propagation. TGBp1 is the main movement protein that physically
CC       cross the plasmodesma with the viral genome. TGBp2 and TGBp3 would
CC       facilitate TGBp1 function.
CC   -!- SIMILARITY: Belongs to the Tymovirales TGBp1 protein family.
CC       {ECO:0000305}.
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DR   EMBL; D14449; BAA03340.1; -; Genomic_RNA.
DR   PIR; PN0094; WMVYPM.
DR   RefSeq; NP_056768.1; NC_001361.2.
DR   SMR; P17526; -.
DR   PRIDE; P17526; -.
DR   GeneID; 1493993; -.
DR   KEGG; vg:1493993; -.
DR   Proteomes; UP000000677; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR   InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR   Pfam; PF01443; Viral_helicase1; 1.
DR   PROSITE; PS51657; PSRV_HELICASE; 1.
PE   3: Inferred from homology;
KW   Host cytoplasm; Host-virus interaction; Reference proteome; RNA-binding;
KW   Suppressor of RNA silencing; Transport; Viral movement protein.
FT   CHAIN           1..229
FT                   /note="Movement and silencing protein TGBp1"
FT                   /id="PRO_0000222581"
FT   DOMAIN          1..132
FT                   /note="(+)RNA virus helicase ATP-binding"
FT   DOMAIN          133..229
FT                   /note="(+)RNA virus helicase C-terminal"
FT   CONFLICT        215..219
FT                   /note="TALHI -> R (in Ref. 1; BAA03340)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        222..229
FT                   /note="PDATYTAA -> TSVS (in Ref. 1; BAA03340)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   229 AA;  25439 MW;  6CFFE8C120915018 CRC64;
     MDVIVDLLYK YKFERLSNKL VCPIVVHCVP GAGKSSLIRE LLELDSRFCA YTAGVEDQPR
     LSGNWIRKWS GQQPEGKFVV LDEYTLLTEV PPVFALFGDP IQSNTSAVQR ADFVCSVSRR
     FGSATCGLLR ELGWNVRSEK ADLVQVSDIY TKDPLGKVVF SEEEVGCLLR SHGVEALSLQ
     EITGQTFEVV TFVTSENSPV INRAAAYQCM TRHRTALHIL CPDATYTAA