ID   TGB1_PVSP               Reviewed;         227 AA.
AC   P16650;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   07-OCT-2020, entry version 65.
DE   RecName: Full=Movement and silencing protein TGBp1;
DE   AltName: Full=25 kDa protein;
DE   AltName: Full=Silencing suppressor P25;
DE   AltName: Full=Triple gene block 1 protein;
DE            Short=TGBp1;
GN   ORFNames=ORF2;
OS   Potato virus S (strain Peruvian).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus.
OX   NCBI_TaxID=12170;
OH   NCBI_TaxID=4113; Solanum tuberosum (Potato).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2732711; DOI=10.1099/0022-1317-70-5-1053;
RA   Mackenzie D.J., Tremaine J.H., Stace-Smith R.;
RT   "Organization and interviral homologies of the 3'-terminal portion of
RT   potato virus S RNA.";
RL   J. Gen. Virol. 70:1053-1063(1989).
CC   -!- FUNCTION: Transports viral genome to neighboring plant cells directly
CC       through plasmosdesmata, without any budding. The movement protein
CC       allows efficient cell to cell propagation, by bypassing the host cell
CC       wall barrier. Increases plasmodesma size exclusion limit. Acts as a
CC       suppressor of RNA-mediated gene silencing, also known as post-
CC       transcriptional gene silencing (PTGS), a mechanism of plant viral
CC       defense that limits the accumulation of viral RNAs (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer and homooligomer. Interacts with capsid protein.
CC       Interacts with host AGO1; this interaction targets the host protein for
CC       degradation, thereby suppressing the antiviral RNA silencing (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250}.
CC   -!- MISCELLANEOUS: TGBp1, TGBp2 and TGBp3 seem to act together for cell-to-
CC       cell propagation. TGBp1 is the main movement protein that physically
CC       cross the plasmodesma with the viral genome. TGBp2 and TGBp3 would
CC       facilitate TGBp1 function.
CC   -!- SIMILARITY: Belongs to the Tymovirales TGBp1 protein family.
CC       {ECO:0000305}.
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DR   EMBL; D00461; BAA00351.1; -; Genomic_RNA.
DR   PIR; JA0124; JA0124.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR   InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR   Pfam; PF01443; Viral_helicase1; 1.
DR   PROSITE; PS51657; PSRV_HELICASE; 1.
PE   3: Inferred from homology;
KW   Host cytoplasm; Host-virus interaction; RNA-binding;
KW   Suppressor of RNA silencing; Transport; Viral movement protein.
FT   CHAIN           1..227
FT                   /note="Movement and silencing protein TGBp1"
FT                   /id="PRO_0000222579"
FT   DOMAIN          1..131
FT                   /note="(+)RNA virus helicase ATP-binding"
FT   DOMAIN          132..227
FT                   /note="(+)RNA virus helicase C-terminal"
SQ   SEQUENCE   227 AA;  25107 MW;  373CAFDCD89C93E2 CRC64;
     MDVFLQVLNK YKFERVSSTL NKPIVVHSVP GAGKSSAIRE LLKLDSRFEC ITRGRPDIPN
     LEGAFIKAER SGESKLLLVD EYIEGPIPED AFAIFADPLQ STAVSPHRAH FIKTLSHRFG
     KCTDSLLRDL GWDVQAEGQD SVQIADIFTV DPRETIVYFE PEVGELLRSH GVEASCIGEV
     RGATFEHVTF VTSENSPLID KASAFQCLTR HTKSLLILCP DATYTAA