TGB1_PVXCP
ID TGB1_PVXCP Reviewed; 226 AA.
AC P22592;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1991, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=Movement and silencing protein TGBp1;
DE AltName: Full=25 kDa protein;
DE AltName: Full=Silencing suppressor P25;
DE AltName: Full=Triple gene block 1 protein;
DE Short=TGBp1;
GN ORFNames=ORF2;
OS Potato virus X (strain CP) (PVX).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Tymovirales; Alphaflexiviridae; Potexvirus.
OX NCBI_TaxID=12184;
OH NCBI_TaxID=3711; Brassica campestris (Field mustard).
OH NCBI_TaxID=4113; Solanum tuberosum (Potato).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=2392880; DOI=10.1016/0168-1702(90)90054-f;
RA Orman B.E., Celnik R.M., Mandel A.M., Torres H.N., Mentaberry A.N.;
RT "Complete cDNA sequence of a South American isolate of potato virus X.";
RL Virus Res. 16:293-305(1990).
RN [2]
RP REVIEW.
RX PubMed=15828680; DOI=10.1094/mpmi-18-0283;
RA Verchot-Lubicz J.;
RT "A new cell-to-cell transport model for Potexviruses.";
RL Mol. Plant Microbe Interact. 18:283-290(2005).
CC -!- FUNCTION: Transports viral genome to neighboring plant cells directly
CC through plasmosdesmata, without any budding. The movement protein
CC allows efficient cell to cell propagation, by bypassing the host cell
CC wall barrier. Increases plasmodesma size exclusion limit. Acts as a
CC suppressor of RNA-mediated gene silencing, also known as post-
CC transcriptional gene silencing (PTGS), a mechanism of plant viral
CC defense that limits the accumulation of viral RNAs (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Homodimer and homooligomer. Interacts with capsid protein.
CC Interacts with host AGO1; this interaction targets the host protein for
CC degradation, thereby suppressing the antiviral RNA silencing (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250}.
CC -!- MISCELLANEOUS: TGBp1, TGBp2 and TGBp3 seem to act together for cell-to-
CC cell propagation. TGBp1 is the main movement protein that physically
CC cross the plasmodesma with the viral genome. TGBp2 and TGBp3 would
CC facilitate TGBp1 function.
CC -!- SIMILARITY: Belongs to the Tymovirales TGBp1 protein family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M31541; AAA47179.1; -; Genomic_RNA.
DR EMBL; X55802; CAA39325.1; -; Genomic_RNA.
DR PIR; S14006; S14006.
DR Proteomes; UP000008615; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR Pfam; PF01443; Viral_helicase1; 1.
DR PROSITE; PS51657; PSRV_HELICASE; 1.
PE 3: Inferred from homology;
KW Host cytoplasm; Host-virus interaction; Reference proteome; RNA-binding;
KW Suppressor of RNA silencing; Transport; Viral movement protein.
FT CHAIN 1..226
FT /note="Movement and silencing protein TGBp1"
FT /id="PRO_0000222571"
FT DOMAIN 1..138
FT /note="(+)RNA virus helicase ATP-binding"
FT DOMAIN 139..226
FT /note="(+)RNA virus helicase C-terminal"
SQ SEQUENCE 226 AA; 24420 MW; 664CD0903C1DAEFC CRC64;
MDILIISLKS LGYSRTARPL DSSPLVVHAV AGAGKSTALR KLLARHSTFT VHTLGVPDKI
SIRTRGIQKP GPIPEGNFAI LDEYTLDATT REAYQALFAD PYQAPELSLE PHFYLETSFR
TPTKAAALIA SCGFDFETNS QEEGHLEITG IFKGPLLGKV IAIDSEAETT LSRHGVEFVK
PCQVTGLEFP VVTIVSAAPI EEIGQSTLFY NAITRSKGLT YVRAGA