ID   TGB1_PVXHB              Reviewed;         226 AA.
AC   Q07632;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   29-SEP-2021, entry version 76.
DE   RecName: Full=Movement and silencing protein TGBp1;
DE   AltName: Full=25 kDa protein;
DE   AltName: Full=Silencing suppressor P25;
DE   AltName: Full=Triple gene block 1 protein;
DE            Short=TGBp1;
GN   ORFNames=ORF2;
OS   Potato virus X (strain HB) (PVX).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Tymovirales; Alphaflexiviridae; Potexvirus.
OX   NCBI_TaxID=73488;
OH   NCBI_TaxID=3711; Brassica campestris (Field mustard).
OH   NCBI_TaxID=4113; Solanum tuberosum (Potato).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=8409947; DOI=10.1099/0022-1317-74-10-2251;
RA   Querci M., van der Vlugt R., Goldbach R., Salazar L.F.;
RT   "RNA sequence of potato virus X strain HB.";
RL   J. Gen. Virol. 74:2251-2255(1993).
RN   [2]
RP   REVIEW.
RX   PubMed=15828680; DOI=10.1094/mpmi-18-0283;
RA   Verchot-Lubicz J.;
RT   "A new cell-to-cell transport model for Potexviruses.";
RL   Mol. Plant Microbe Interact. 18:283-290(2005).
CC   -!- FUNCTION: Transports viral genome to neighboring plant cells directly
CC       through plasmosdesmata, without any budding. The movement protein
CC       allows efficient cell to cell propagation, by bypassing the host cell
CC       wall barrier. Increases plasmodesma size exclusion limit. Acts as a
CC       suppressor of RNA-mediated gene silencing, also known as post-
CC       transcriptional gene silencing (PTGS), a mechanism of plant viral
CC       defense that limits the accumulation of viral RNAs (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer and homooligomer. Interacts with capsid protein.
CC       Interacts with host AGO1; this interaction targets the host protein for
CC       degradation, thereby suppressing the antiviral RNA silencing (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250}.
CC   -!- MISCELLANEOUS: TGBp1, TGBp2 and TGBp3 seem to act together for cell-to-
CC       cell propagation. TGBp1 is the main movement protein that physically
CC       cross the plasmodesma with the viral genome. TGBp2 and TGBp3 would
CC       facilitate TGBp1 function.
CC   -!- SIMILARITY: Belongs to the Tymovirales TGBp1 protein family.
CC       {ECO:0000305}.
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DR   EMBL; X72214; CAA51013.1; -; Genomic_RNA.
DR   PIR; JQ2295; JQ2295.
DR   Proteomes; UP000006842; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR   InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR   Pfam; PF01443; Viral_helicase1; 1.
DR   PROSITE; PS51657; PSRV_HELICASE; 1.
PE   3: Inferred from homology;
KW   Host cytoplasm; Host-virus interaction; Reference proteome; RNA-binding;
KW   Suppressor of RNA silencing; Transport; Viral movement protein.
FT   CHAIN           1..226
FT                   /note="Movement and silencing protein TGBp1"
FT                   /id="PRO_0000222572"
FT   DOMAIN          1..138
FT                   /note="(+)RNA virus helicase ATP-binding"
FT   DOMAIN          139..226
FT                   /note="(+)RNA virus helicase C-terminal"
SQ   SEQUENCE   226 AA;  24462 MW;  DA1D21CD5B94461F CRC64;
     MDILIISLKS LGYSRTSRPL DSGPLVVHAV AGAGKSTALR KLLARHSTFT IHTLGVPDKI
     SIRTRGIQKP GPIPKGNFAI LDEYTLDATT REAYQALFAD PYQAPELSLE PHFYLETSFR
     TPKKAAALIA SCGFDFETNS QEEGHLEVTG IFKGPLLGKV IAIDSEAETT LSRHGVEFVK
     PCQVTGLEFP VVTIVSAAPI EEIGQSTLFY NAITRSKGLT YVRAGT