TGB1_PVXX3
ID TGB1_PVXX3 Reviewed; 226 AA.
AC P17780;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 23-FEB-2022, entry version 77.
DE RecName: Full=Movement and silencing protein TGBp1;
DE AltName: Full=25 kDa protein;
DE AltName: Full=Silencing suppressor P25;
DE AltName: Full=Triple gene block 1 protein;
DE Short=TGBp1;
GN ORFNames=ORF2;
OS Potato virus X (strain X3) (PVX).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Tymovirales; Alphaflexiviridae; Potexvirus.
OX NCBI_TaxID=12185;
OH NCBI_TaxID=3711; Brassica campestris (Field mustard).
OH NCBI_TaxID=4113; Solanum tuberosum (Potato).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=3404114; DOI=10.1099/0022-1317-69-8-1789;
RA Huisman M.J., Linthorst H.J.M., Bol J.F., Cornelissen B.J.C.;
RT "The complete nucleotide sequence of potato virus X and its homologies at
RT the amino acid level with various plus-stranded RNA viruses.";
RL J. Gen. Virol. 69:1789-1798(1988).
RN [2]
RP RNA-BINDING.
RX PubMed=10405356; DOI=10.1006/viro.1999.9788;
RA Morozov S.Y., Solovyev A.G., Kalinina N.O., Fedorkin O.N., Samuilova O.V.,
RA Schiemann J., Atabekov J.G.;
RT "Evidence for two nonoverlapping functional domains in the potato virus X
RT 25K movement protein.";
RL Virology 260:55-63(1999).
RN [3]
RP FUNCTION.
RX PubMed=15464839; DOI=10.1016/j.virol.2004.06.039;
RA Howard A.R., Heppler M.L., Ju H.J., Krishnamurthy K., Payton M.E.,
RA Verchot-Lubicz J.;
RT "Potato virus X TGBp1 induces plasmodesmata gating and moves between cells
RT in several host species whereas CP moves only in N. benthamiana leaves.";
RL Virology 328:185-197(2004).
RN [4]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH CAPSID PROTEIN.
RX PubMed=17610926; DOI=10.1016/j.virol.2007.05.022;
RA Samuels T.D., Ju H.J., Ye C.M., Motes C.M., Blancaflor E.B.,
RA Verchot-Lubicz J.;
RT "Subcellular targeting and interactions among the Potato virus X TGB
RT proteins.";
RL Virology 367:375-389(2007).
RN [5]
RP HOMODIMERIZATION, AND HOMOOLIGOMERIZATION.
RX PubMed=18294129; DOI=10.1134/s0006297908010070;
RA Leshchiner A.D., Minina E.A., Rakitina D.V., Vishnichenko V.K.,
RA Solovyev A.G., Morozov S.Y., Kalinina N.O.;
RT "Oligomerization of the potato virus X 25-kD movement protein.";
RL Biochemistry (Mosc.) 73:50-55(2008).
RN [6]
RP FUNCTION.
RX PubMed=20696002; DOI=10.1111/j.1364-3703.2010.00634.x;
RA Chiu M.H., Chen I.H., Baulcombe D.C., Tsai C.H.;
RT "The silencing suppressor P25 of Potato virus X interacts with Argonaute1
RT and mediates its degradation through the proteasome pathway.";
RL Mol. Plant Pathol. 11:641-649(2010).
RN [7]
RP REVIEW.
RX PubMed=15828680; DOI=10.1094/mpmi-18-0283;
RA Verchot-Lubicz J.;
RT "A new cell-to-cell transport model for Potexviruses.";
RL Mol. Plant Microbe Interact. 18:283-290(2005).
CC -!- FUNCTION: Transports viral genome to neighboring plant cells directly
CC through plasmosdesmata, without any budding. The movement protein
CC allows efficient cell to cell propagation, by bypassing the host cell
CC wall barrier. Increases plasmodesma size exclusion limit. Acts as a
CC suppressor of RNA-mediated gene silencing, also known as post-
CC transcriptional gene silencing (PTGS), a mechanism of plant viral
CC defense that limits the accumulation of viral RNAs.
CC {ECO:0000269|PubMed:15464839, ECO:0000269|PubMed:20696002}.
CC -!- SUBUNIT: Homodimer and homooligomer. Interacts with capsid protein.
CC Interacts with host AGO1; this interaction targets the host protein for
CC degradation, thereby suppressing the antiviral RNA silencing.
CC {ECO:0000269|PubMed:17610926}.
CC -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000269|PubMed:17610926}.
CC -!- MISCELLANEOUS: TGBp1, TGBp2 and TGBp3 seem to act together for cell-to-
CC cell propagation. TGBp1 is the main movement protein that physically
CC cross the plasmodesma with the viral genome. TGBp2 and TGBp3 would
CC facilitate TGBp1 function.
CC -!- SIMILARITY: Belongs to the Tymovirales TGBp1 protein family.
CC {ECO:0000305}.
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DR EMBL; D00344; BAA00250.1; -; Genomic_RNA.
DR PIR; JA0103; WMWGP2.
DR RefSeq; YP_002332930.1; NC_011620.1.
DR GeneID; 7065755; -.
DR KEGG; vg:7065755; -.
DR Proteomes; UP000008687; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR Pfam; PF01443; Viral_helicase1; 1.
DR PROSITE; PS51657; PSRV_HELICASE; 1.
PE 1: Evidence at protein level;
KW Host cytoplasm; Host-virus interaction; Reference proteome; RNA-binding;
KW Suppressor of RNA silencing; Transport; Viral movement protein.
FT CHAIN 1..226
FT /note="Movement and silencing protein TGBp1"
FT /id="PRO_0000222573"
FT DOMAIN 1..115
FT /note="(+)RNA virus helicase ATP-binding"
FT DOMAIN 116..226
FT /note="(+)RNA virus helicase C-terminal"
SQ SEQUENCE 226 AA; 24596 MW; C9CD64335D061931 CRC64;
MDILISSLKS LGYSRTSKSL DSGPLVVHAV AGAGKSTALR KLILRHPTFT VHTLGVPDKV
SIRTRGIQKP GPIPEGNFAI LDEYTLDNTT RNSYQALFAD PYQAPEFSLE PHFYLETSFR
VPRKVADLIA GCGFDFETNS QEEGHLEITG IFKGPLLGKV IAIDEESETT LSRHGVEFVK
PCQVTGLELK VVTIVSAAPI EEIGQSTAFY NAITRSKGLT YVRAGT
