ID   TGB1_WCMVO              Reviewed;         236 AA.
AC   P15403;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Movement and silencing protein TGBp1;
DE   AltName: Full=25 kDa protein;
DE   AltName: Full=Silencing suppressor P25;
DE   AltName: Full=Triple gene block 1 protein;
DE            Short=TGBp1;
GN   ORFNames=ORF2;
OS   White clover mosaic virus (strain O) (WCMV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC   Tymovirales; Alphaflexiviridae; Potexvirus.
OX   NCBI_TaxID=12190;
OH   NCBI_TaxID=3898; Trifolium.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2353451; DOI=10.1016/0042-6822(90)90469-8;
RA   Beck D.L., Forster R.L.S., Bevan M.W., Boxen K.A., Lowe S.C., Gardner R.C.;
RT   "Infectious transcripts and nucleotide sequence of cloned cDNA of the
RT   potexvirus white clover mosaic virus.";
RL   Virology 177:152-158(1990).
RN   [2]
RP   REVIEW.
RX   PubMed=15828680; DOI=10.1094/mpmi-18-0283;
RA   Verchot-Lubicz J.;
RT   "A new cell-to-cell transport model for Potexviruses.";
RL   Mol. Plant Microbe Interact. 18:283-290(2005).
CC   -!- FUNCTION: Transports viral genome to neighboring plant cells directly
CC       through plasmosdesmata, without any budding. The movement protein
CC       allows efficient cell to cell propagation, by bypassing the host cell
CC       wall barrier. Increases plasmodesma size exclusion limit. Acts as a
CC       suppressor of RNA-mediated gene silencing, also known as post-
CC       transcriptional gene silencing (PTGS), a mechanism of plant viral
CC       defense that limits the accumulation of viral RNAs (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer and homooligomer. Interacts with capsid protein.
CC       Interacts with host AGO1; this interaction targets the host protein for
CC       degradation, thereby suppressing the antiviral RNA silencing (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250}.
CC   -!- MISCELLANEOUS: TGBp1, TGBp2 and TGBp3 seem to act together for cell-to-
CC       cell propagation. TGBp1 is the main movement protein that physically
CC       cross the plasmodesma with the viral genome. TGBp2 and TGBp3 would
CC       facilitate TGBp1 function.
CC   -!- SIMILARITY: Belongs to the Tymovirales TGBp1 protein family.
CC       {ECO:0000305}.
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DR   EMBL; X16636; CAA34629.1; -; Genomic_RNA.
DR   PIR; B46350; B46350.
DR   SMR; P15403; -.
DR   Proteomes; UP000007628; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR   InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01443; Viral_helicase1; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51657; PSRV_HELICASE; 1.
PE   3: Inferred from homology;
KW   Host cytoplasm; Host-virus interaction; Reference proteome; RNA-binding;
KW   Suppressor of RNA silencing; Transport; Viral movement protein.
FT   CHAIN           1..236
FT                   /note="Movement and silencing protein TGBp1"
FT                   /id="PRO_0000222576"
FT   DOMAIN          1..117
FT                   /note="(+)RNA virus helicase ATP-binding"
FT   DOMAIN          118..236
FT                   /note="(+)RNA virus helicase C-terminal"
SQ   SEQUENCE   236 AA;  26451 MW;  D470D75B46E9D418 CRC64;
     MDHIHHLLSS HGFTRTRLAK SKPIVVHAIA GSGKSTVIRK ILSDLPNARA YTLGKPDPYS
     LSNPTIKAFA QFKRGTLDIL DEYGQLPFAD LDSSFEFIFT DPYQAPTDNL FEPHYTLEIT
     YRFGPNTCNL LNQAFQSNIT SLVTQDNISF GSPYLVDPVG TILAFQPDTY LILCLHQAPF
     FKVSEVIGYQ WPTVTLYLAC KISEIPEEER HLLFIGLTRH TESLLILGPD AFDSSP