TGB2_BSMV
ID TGB2_BSMV Reviewed; 204 AA.
AC P04869;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 31-MAY-2011, sequence version 2.
DT 23-FEB-2022, entry version 58.
DE RecName: Full=Movement protein TGB2;
DE AltName: Full=14 kDa protein;
DE AltName: Full=Beta-D protein;
DE AltName: Full=Triple gene block 2 protein;
DE Short=TGBp2;
DE Contains:
DE RecName: Full=TGB2' protein;
OS Barley stripe mosaic virus (BSMV).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Martellivirales; Virgaviridae; Hordeivirus.
OX NCBI_TaxID=12327;
OH NCBI_TaxID=4513; Hordeum vulgare (Barley).
OH NCBI_TaxID=4565; Triticum aestivum (Wheat).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA], READTHROUGH, AND IDENTIFICATION OF
RP TGB2'.
RC STRAIN=ATCC PV43;
RX PubMed=3754962; DOI=10.1093/nar/14.9.3895;
RA Gustafson G., Armour S.L.;
RT "The complete nucleotide sequence of RNA beta from the type strain of
RT barley stripe mosaic virus.";
RL Nucleic Acids Res. 14:3895-3909(1986).
RN [2]
RP INTERACTION WITH MOVEMENT PROTEIN TGB3, AND FUNCTION.
RX PubMed=18353960; DOI=10.1128/jvi.02586-07;
RA Lim H.S., Bragg J.N., Ganesan U., Lawrence D.M., Yu J., Isogai M.,
RA Hammond J., Jackson A.O.;
RT "Triple gene block protein interactions involved in movement of Barley
RT stripe mosaic virus.";
RL J. Virol. 82:4991-5006(2008).
RN [3]
RP SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=19570874; DOI=10.1128/jvi.00739-09;
RA Lim H.S., Bragg J.N., Ganesan U., Ruzin S., Schichnes D., Lee M.Y.,
RA Vaira A.M., Ryu K.H., Hammond J., Jackson A.O.;
RT "Subcellular localization of the barley stripe mosaic virus triple gene
RT block proteins.";
RL J. Virol. 83:9432-9448(2009).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=25288925; DOI=10.5423/ppj.oa.09.2012.0144;
RA Lim H.S., Lee M.Y., Moon J.S., Moon J.K., Yu Y.M., Cho I.S., Bae H.,
RA deBoer M., Ju H., Hammond J., Jackson A.O.;
RT "Actin Cytoskeleton and Golgi Involvement in Barley stripe mosaic virus
RT Movement and Cell Wall Localization of Triple Gene Block Proteins.";
RL Plant Pathol. J. 29:17-30(2013).
RN [5]
RP IDENTIFICATION IN THE TGB1-TGB2-TGB3 COMPLEX.
RX PubMed=32730331; DOI=10.1371/journal.ppat.1008709;
RA Jiang Z., Zhang K., Li Z., Li Z., Yang M., Jin X., Cao Q., Wang X., Yue N.,
RA Li D., Zhang Y.;
RT "The Barley stripe mosaic virus gammab protein promotes viral cell-to-cell
RT movement by enhancing ATPase-mediated assembly of ribonucleoprotein
RT movement complexes.";
RL PLoS Pathog. 16:e1008709-e1008709(2020).
CC -!- FUNCTION: Participates in the transport of viral genome to neighboring
CC plant cells directly through plasmodesmata, without any budding
CC (PubMed:18353960). TGBp2 and TGBp3 are necessary for intracellular
CC delivery of TGBp1-containing vRNPs to plasmodesmata (PubMed:19570874).
CC Can gate plasmodesmata and increase their size exclusion limit
CC (PubMed:19570874). To a lesser extent than TGB3, induces host actin
CC cytoskeleton network thickening, which probably plays a major role in
CC virus cell-to-cell movement (PubMed:25288925).
CC {ECO:0000269|PubMed:18353960, ECO:0000269|PubMed:19570874,
CC ECO:0000269|PubMed:25288925}.
CC -!- SUBUNIT: Interacts with movement protein TGB3 (PubMed:18353960). TGB1-
CC TGB3-TGB2 complex formation is enhanced by ATP hydrolysis
CC (PubMed:32730331). {ECO:0000269|PubMed:18353960,
CC ECO:0000269|PubMed:32730331}.
CC -!- SUBCELLULAR LOCATION: Host cell junction, host plasmodesma
CC {ECO:0000269|PubMed:19570874}. Host endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:25288925}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q9IV53}. Host cytoplasm, host cytoskeleton
CC {ECO:0000269|PubMed:25288925}. Note=Probably localizes to
CC plasmodesmata-associated membrane compartments called peripheral
CC membrane bodies (PMBs) (Probable). Associates with host actin
CC filaments, possibly for the intracellular transport to the
CC plasmodesmata (PubMed:25288925). The viral replication sites are
CC located at the host chloroplast membrane (By similarity).
CC {ECO:0000250|UniProtKB:Q9IV52, ECO:0000269|PubMed:25288925,
CC ECO:0000305}.
CC -!- MISCELLANEOUS: This protein is translated as a fusion protein by
CC episodic readthrough of a termination codon. Readthrough of the
CC terminator codon TGA occurs between the codons for Gly-131 and Ser-133,
CC thereby producing TGB2' minor translational readthrough protein.
CC -!- MISCELLANEOUS: The genome of this virus consists of three linear,
CC positive, single-stranded RNAs encapsidated in separate virions
CC designated RNA-alpha, RNA-beta and RNA-gamma. Three proteins (alpha-A,
CC beta-A and gamma-A) are translated directly from these genomic RNAs and
CC the remaining proteins encoded on RNA-beta (beta-B, beta-C and beta-D)
CC and RNA-gamma (gamma-B) are expressed via three subgenomic messenger
CC RNAs. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the virgaviridae/benyvirus TGB2 movement protein
CC family. {ECO:0000305}.
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DR EMBL; X03854; CAA27486.1; -; Genomic_RNA.
DR PIR; A04192; WMBV4B.
DR RefSeq; NP_604488.1; NC_003481.1.
DR GeneID; 962678; -.
DR KEGG; vg:962678; -.
DR Proteomes; UP000001667; Genome.
DR GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044219; C:host cell plasmodesma; IEA:UniProtKB-SubCell.
DR GO; GO:0044163; C:host cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR InterPro; IPR001896; Plant_vir_prot.
DR Pfam; PF01307; Plant_vir_prot; 1.
PE 1: Evidence at protein level;
KW Host cell junction; Host cytoplasm; Host cytoskeleton;
KW Host endoplasmic reticulum; Host membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport; Viral movement protein.
FT CHAIN 1..204
FT /note="TGB2' protein"
FT /id="PRO_0000409145"
FT CHAIN 1..131
FT /note="Movement protein TGB2"
FT /id="PRO_0000222489"
FT TOPO_DOM 1..11
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9IV53"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:Q9IV53"
FT TOPO_DOM 33..72
FT /note="Lumenal"
FT /evidence="ECO:0000250|UniProtKB:Q9IV53"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:Q9IV53"
FT TOPO_DOM 94..204
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9IV53"
SQ SEQUENCE 204 AA; 22670 MW; A419113D11669EE7 CRC64;
MKTTVGSRPN KYWPIVAGIG VVGLFAYLIF SNQKHSTESG DNIHKFANGG SYRDGSKSIS
YNRNHPFAYG NASSPGMLLP AMLTIIGIIS YLWRTRDSVL GDSGGNNSCG EDCQGECLNG
HSRRSLLCDI GXSFYHCSMA IVYISKQYTY GDWSLLLSRS ELCEDLWNRG YEPRSYCGHP
PLAEVPFWGI SDVGRFNQCF EYSS
