TGB2_PMTVS
ID TGB2_PMTVS Reviewed; 119 AA.
AC Q9IV53;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 23-FEB-2022, entry version 39.
DE RecName: Full=Movement protein TGB2;
DE AltName: Full=P14;
DE AltName: Full=Triple gene block 2 protein;
DE Short=TGBp2;
OS Potato mop-top virus (isolate Potato/Sweden/Sw) (PMTV).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Martellivirales; Virgaviridae; Pomovirus.
OX NCBI_TaxID=652839;
OH NCBI_TaxID=4112; Solanum nigrum (Black nightshade).
OH NCBI_TaxID=4113; Solanum tuberosum (Potato).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=12655103; DOI=10.1099/vir.0.18813-0;
RA Savenkov E.I., Germundsson A., Zamyatnin A.A. Jr., Sandgren M.,
RA Valkonen J.P.;
RT "Potato mop-top virus: the coat protein-encoding RNA and the gene for
RT cysteine-rich protein are dispensable for systemic virus movement in
RT Nicotiana benthamiana.";
RL J. Gen. Virol. 84:1001-1005(2003).
RN [2]
RP RNA-BINDING, INTERACTION WITH TGB3, AND SUBCELLULAR LOCATION.
RX PubMed=12093178; DOI=10.1006/viro.2002.1435;
RA Cowan G.H., Lioliopoulou F., Ziegler A., Torrance L.;
RT "Subcellular localisation, protein interactions, and RNA binding of Potato
RT mop-top virus triple gene block proteins.";
RL Virology 298:106-115(2002).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH TGB3.
RX PubMed=15608333; DOI=10.1105/tpc.104.027821;
RA Haupt S., Cowan G.H., Ziegler A., Roberts A.G., Oparka K.J., Torrance L.;
RT "Two plant-viral movement proteins traffic in the endocytic recycling
RT pathway.";
RL Plant Cell 17:164-181(2005).
RN [4]
RP TOPOLOGY.
RX PubMed=16553902; DOI=10.1111/j.1365-313x.2006.02674.x;
RA Zamyatnin A.A. Jr., Solovyev A.G., Bozhkov P.V., Valkonen J.P.,
RA Morozov S.Y., Savenkov E.I.;
RT "Assessment of the integral membrane protein topology in living cells.";
RL Plant J. 46:145-154(2006).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=23269927; DOI=10.3389/fpls.2012.00290;
RA Cowan G.H., Roberts A.G., Chapman S.N., Ziegler A., Savenkov E.I.,
RA Torrance L.;
RT "The potato mop-top virus TGB2 protein and viral RNA associate with
RT chloroplasts and viral infection induces inclusions in the plastids.";
RL Front. Plant Sci. 3:290-290(2012).
CC -!- FUNCTION: Participates in the transport of viral genome to neighboring
CC plant cells directly through plasmodesmata, without any budding. TGBp2
CC and TGBp3 are necessary for intracellular delivery of TGBp1-containing
CC vRNPs to plasmodesmata. Can gate plasmodesmata and increase their size
CC exclusion limit. To a lesser extent than TGB3, induces host actin
CC cytoskeleton network thickening, which probably plays a major role in
CC virus cell-to-cell movement (By similarity). Binds ssRNA in a sequence
CC non-specific manner (PubMed:12093178). {ECO:0000250|UniProtKB:P04869,
CC ECO:0000269|PubMed:12093178}.
CC -!- SUBUNIT: Interacts with movement protein TGB3 (PubMed:12093178,
CC PubMed:15608333). TGB1-TGB3-TGB2 complex formation is enhanced by ATP
CC hydrolysis (By similarity). {ECO:0000250|UniProtKB:P04869,
CC ECO:0000269|PubMed:12093178, ECO:0000269|PubMed:15608333}.
CC -!- SUBCELLULAR LOCATION: Host cell junction, host plasmodesma
CC {ECO:0000305|PubMed:12093178}. Host endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:15608333, ECO:0000269|PubMed:23269927}; Multi-pass
CC membrane protein {ECO:0000269|PubMed:16553902}. Host cytoplasm, host
CC cytoskeleton {ECO:0000250|UniProtKB:P04869}. Host chloroplast envelope
CC {ECO:0000269|PubMed:23269927}. Note=Probably localizes to
CC plasmodesmata-associated membrane compartments called peripheral
CC membrane bodies (PMBs). Associates with host actin filaments, possibly
CC for the intracellular transport to the plasmodesmata (By similarity).
CC The viral replication sites are located at the host chloroplast
CC membrane (PubMed:23269927). {ECO:0000250|UniProtKB:P04869,
CC ECO:0000269|PubMed:23269927}.
CC -!- SIMILARITY: Belongs to the virgaviridae/benyvirus TGB2 movement protein
CC family. {ECO:0000305}.
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DR EMBL; AJ277556; CAB91102.1; -; Genomic_RNA.
DR RefSeq; NP_620439.1; NC_003725.1.
DR TCDB; 9.B.308.6.1; the lettuce infectious yellows virus p5 (liyv-p5) family.
DR GeneID; 991175; -.
DR KEGG; vg:991175; -.
DR Proteomes; UP000006715; Genome.
DR GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044219; C:host cell plasmodesma; IEA:UniProtKB-SubCell.
DR GO; GO:0044163; C:host cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR InterPro; IPR001896; Plant_vir_prot.
DR Pfam; PF01307; Plant_vir_prot; 1.
PE 1: Evidence at protein level;
KW Host cell junction; Host cytoplasm; Host cytoskeleton;
KW Host endoplasmic reticulum; Host membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport; Viral movement protein.
FT CHAIN 1..119
FT /note="Movement protein TGB2"
FT /id="PRO_0000410610"
FT TOPO_DOM 1..13
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:16553902"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000305|PubMed:16553902"
FT TOPO_DOM 35..79
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:16553902"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000305|PubMed:16553902"
FT TOPO_DOM 101..119
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:16553902"
SQ SEQUENCE 119 AA; 13123 MW; CF15C8BC03305932 CRC64;
MVRNNEIGAR PNKYWPVVAA VVAICLFGFL TVTNQKHATQ SGDNIHKFAN GGQYRDGSKS
IKYNCNNPRA YNGSSSNITF SQLFLPVLLI GAALYAYLWF TRPDCSVTCR GDCCRSYGG
