TGB3_BSMV
ID TGB3_BSMV Reviewed; 155 AA.
AC P04868;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 13-AUG-1987, sequence version 1.
DT 23-FEB-2022, entry version 59.
DE RecName: Full=Movement protein TGB3;
DE AltName: Full=17 kDa protein;
DE AltName: Full=Beta-C protein;
DE AltName: Full=Triple gene block 3 protein;
DE Short=TGBp3;
OS Barley stripe mosaic virus (BSMV).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Martellivirales; Virgaviridae; Hordeivirus.
OX NCBI_TaxID=12327;
OH NCBI_TaxID=4513; Hordeum vulgare (Barley).
OH NCBI_TaxID=4565; Triticum aestivum (Wheat).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=ATCC PV43;
RX PubMed=3754962; DOI=10.1093/nar/14.9.3895;
RA Gustafson G., Armour S.L.;
RT "The complete nucleotide sequence of RNA beta from the type strain of
RT barley stripe mosaic virus.";
RL Nucleic Acids Res. 14:3895-3909(1986).
RN [2]
RP INTERACTION WITH MOVEMENT PROTEIN TGB1, INTERACTION WITH MOVEMENT PROTEIN
RP TGB2, AND FUNCTION.
RX PubMed=18353960; DOI=10.1128/jvi.02586-07;
RA Lim H.S., Bragg J.N., Ganesan U., Lawrence D.M., Yu J., Isogai M.,
RA Hammond J., Jackson A.O.;
RT "Triple gene block protein interactions involved in movement of Barley
RT stripe mosaic virus.";
RL J. Virol. 82:4991-5006(2008).
RN [3]
RP SUBCELLULAR LOCATION, MUTAGENESIS OF ARG-155, AND FUNCTION.
RX PubMed=19570874; DOI=10.1128/jvi.00739-09;
RA Lim H.S., Bragg J.N., Ganesan U., Ruzin S., Schichnes D., Lee M.Y.,
RA Vaira A.M., Ryu K.H., Hammond J., Jackson A.O.;
RT "Subcellular localization of the barley stripe mosaic virus triple gene
RT block proteins.";
RL J. Virol. 83:9432-9448(2009).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=25288925; DOI=10.5423/ppj.oa.09.2012.0144;
RA Lim H.S., Lee M.Y., Moon J.S., Moon J.K., Yu Y.M., Cho I.S., Bae H.,
RA deBoer M., Ju H., Hammond J., Jackson A.O.;
RT "Actin Cytoskeleton and Golgi Involvement in Barley stripe mosaic virus
RT Movement and Cell Wall Localization of Triple Gene Block Proteins.";
RL Plant Pathol. J. 29:17-30(2013).
RN [5]
RP IDENTIFICATION IN THE TGB1-TGB2-TGB3 COMPLEX.
RX PubMed=32730331; DOI=10.1371/journal.ppat.1008709;
RA Jiang Z., Zhang K., Li Z., Li Z., Yang M., Jin X., Cao Q., Wang X., Yue N.,
RA Li D., Zhang Y.;
RT "The Barley stripe mosaic virus gammab protein promotes viral cell-to-cell
RT movement by enhancing ATPase-mediated assembly of ribonucleoprotein
RT movement complexes.";
RL PLoS Pathog. 16:e1008709-e1008709(2020).
CC -!- FUNCTION: Participates in the transport of viral genome to neighboring
CC plant cells directly through plasmodesmata, without any budding
CC (PubMed:18353960). TGBp2 and TGBp3 are necessary for intracellular
CC delivery of TGBp1-containing vRNPs to plasmodesmata (Probable). Can
CC gate plasmodesmata and increase their size exclusion limit
CC (PubMed:19570874). Induces host actin cytoskeleton network thickening,
CC which probably plays a major role in virus cell-to-cell movement
CC (PubMed:25288925). {ECO:0000269|PubMed:18353960,
CC ECO:0000269|PubMed:19570874, ECO:0000269|PubMed:25288925,
CC ECO:0000305|PubMed:19570874}.
CC -!- SUBUNIT: Interacts with movement proteins TGB1 and TGB2
CC (PubMed:18353960). TGB1-TGB3-TGB2 complex formation is enhanced by ATP
CC hydrolysis (PubMed:32730331). {ECO:0000269|PubMed:18353960,
CC ECO:0000269|PubMed:32730331}.
CC -!- SUBCELLULAR LOCATION: Host cell junction, host plasmodesma
CC {ECO:0000269|PubMed:19570874}. Host endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:25288925}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q9IV52}. Host cytoplasm, host cytoskeleton
CC {ECO:0000269|PubMed:25288925}. Note=Probably localizes to
CC plasmodesmata-associated membrane compartments called peripheral
CC membrane bodies (PMBs) (PubMed:19570874). Associates with host actin
CC filaments (PubMed:25288925). {ECO:0000269|PubMed:19570874,
CC ECO:0000269|PubMed:25288925}.
CC -!- DOMAIN: The 2nd transmembrane domain is involved in plasmodesmata
CC targeting. {ECO:0000250|UniProtKB:Q9IV52}.
CC -!- MISCELLANEOUS: The genome of this virus consists of three linear,
CC positive, single-stranded RNAs encapsidated in separate virions
CC designated RNA-alpha, RNA-beta and RNA-gamma. Three proteins (alpha-A,
CC beta-A and gamma-A) are translated directly from these genomic RNAs and
CC the remaining proteins encoded on RNA-beta (beta-B, beta-C and beta-D)
CC and RNA-gamma (gamma-B) are expressed via three subgenomic messenger
CC RNAs. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the virgaviridae TGB3 movement protein family.
CC {ECO:0000305}.
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DR EMBL; X03854; CAA27487.1; -; Genomic_RNA.
DR PIR; A04193; WMBV7B.
DR RefSeq; NP_604489.1; NC_003481.1.
DR GeneID; 962679; -.
DR KEGG; vg:962679; -.
DR Proteomes; UP000001667; Genome.
DR GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0044219; C:host cell plasmodesma; IEA:UniProtKB-SubCell.
DR GO; GO:0044163; C:host cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR InterPro; IPR007617; Viral_beta_CD.
DR Pfam; PF04530; Viral_Beta_CD; 1.
PE 1: Evidence at protein level;
KW Host cell junction; Host cytoplasm; Host cytoskeleton;
KW Host endoplasmic reticulum; Host membrane; Membrane; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport;
KW Viral movement protein.
FT CHAIN 1..155
FT /note="Movement protein TGB3"
FT /id="PRO_0000222490"
FT TOPO_DOM 1..59
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9IV52"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:Q9IV52"
FT TOPO_DOM 81..130
FT /note="Lumenal"
FT /evidence="ECO:0000250|UniProtKB:Q9IV52"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:Q9IV52"
FT TOPO_DOM 152..155
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9IV52"
FT REGION 150..155
FT /note="Required for attachment to the host plasmodesmata-
FT associated membrane compartments"
FT /evidence="ECO:0000305|PubMed:19570874"
FT MOTIF 89..93
FT /note="Involved in plasmodesmata targeting and virus cell-
FT to-cell movement"
FT /evidence="ECO:0000250|UniProtKB:Q9IV52"
FT MUTAGEN 155
FT /note="R->A: Reduced cell-to-cell movement."
FT /evidence="ECO:0000269|PubMed:19570874"
SQ SEQUENCE 155 AA; 17377 MW; 0AA04829728D027B CRC64;
MAMPHPLECC CPQCLPSSES FPIYGEQEIP CSETQAETTP VEKTVRANVL TDILDDHYYA
ILASLFIIAL WLLYIYLSSI PTETGPYFYQ DLNSVKIYGI GATNPEVIAA IHHWQKYPFG
ESPMWGGLIS VLSILLKPLT LVFALSFFLL LSSKR
